ID A0A242HME5_9ENTE Unreviewed; 1177 AA. AC A0A242HME5; DT 25-OCT-2017, integrated into UniProtKB/TrEMBL. DT 25-OCT-2017, sequence version 1. DT 28-FEB-2018, entry version 5. DE RecName: Full=Pyruvate-flavodoxin oxidoreductase {ECO:0000256|PIRNR:PIRNR000159}; DE EC=1.2.7.- {ECO:0000256|PIRNR:PIRNR000159}; GN ORFNames=A5865_000913 {ECO:0000313|EMBL:OTO77039.1}; OS Enterococcus sp. 12E11_DIV0728. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; OC Enterococcus. OX NCBI_TaxID=1834168 {ECO:0000313|EMBL:OTO77039.1, ECO:0000313|Proteomes:UP000194680}; RN [1] {ECO:0000313|EMBL:OTO77039.1, ECO:0000313|Proteomes:UP000194680} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=12E11_DIV0728 {ECO:0000313|EMBL:OTO77039.1, RC ECO:0000313|Proteomes:UP000194680}; RG The Broad Institute Genomics Platform; RG The Broad Institute Genomic Center for Infectious Diseases; RA Earl A., Manson A., Schwartman J., Gilmore M., Abouelleil A., Cao P., RA Chapman S., Cusick C., Shea T., Young S., Neafsey D., Nusbaum C., RA Birren B.; RT "The Genome Sequence of Enterococcus sp. 12E11_DIV0728."; RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Oxidoreductase required for the transfer of electrons CC from pyruvate to flavodoxin. {ECO:0000256|PIRNR:PIRNR000159}. CC -!- CATALYTIC ACTIVITY: Pyruvate + CoA + oxidized flavodoxin = acetyl- CC CoA + CO(2) + reduced flavodoxin. {ECO:0000256|PIRNR:PIRNR000159}. CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|PIRSR:PIRSR000159-50}; CC Note=Binds 3 [4Fe-4S] clusters per subunit. CC {ECO:0000256|PIRSR:PIRSR000159-50}; CC -!- SIMILARITY: Belongs to the nifJ family. CC {ECO:0000256|PIRNR:PIRNR000159}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:OTO77039.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NGLY01000001; OTO77039.1; -; Genomic_DNA. DR Proteomes; UP000194680; Unassembled WGS sequence. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0005506; F:iron ion binding; IEA:InterPro. DR GO; GO:0016903; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors; IEA:InterPro. DR GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro. DR GO; GO:0022900; P:electron transport chain; IEA:InterPro. DR Gene3D; 3.40.50.920; -; 1. DR Gene3D; 3.40.920.10; -; 1. DR InterPro; IPR017896; 4Fe4S_Fe-S-bd. DR InterPro; IPR017900; 4Fe4S_Fe_S_CS. DR InterPro; IPR033412; PFOR_II. DR InterPro; IPR019456; Pyrv-flavodox_OxRtase_EKR. DR InterPro; IPR019752; Pyrv/ketoisovalerate_OxRed_cat. DR InterPro; IPR002880; Pyrv_Fd/Flavodoxin_OxRdtase_N. DR InterPro; IPR011895; Pyrv_flavodox_OxRed. DR InterPro; IPR002869; Pyrv_flavodox_OxRed_cen. DR InterPro; IPR029061; THDP-binding. DR InterPro; IPR011766; TPP_enzyme-bd_C. DR InterPro; IPR009014; Transketo_C/PFOR_II. DR Pfam; PF10371; EKR; 1. DR Pfam; PF17147; PFOR_II; 1. DR Pfam; PF01558; POR; 1. DR Pfam; PF01855; POR_N; 1. DR Pfam; PF02775; TPP_enzyme_C; 1. DR PIRSF; PIRSF000159; NifJ; 1. DR SMART; SM00890; EKR; 1. DR SUPFAM; SSF52518; SSF52518; 2. DR SUPFAM; SSF52922; SSF52922; 1. DR SUPFAM; SSF53323; SSF53323; 1. DR TIGRFAMs; TIGR02176; pyruv_ox_red; 1. DR PROSITE; PS00198; 4FE4S_FER_1; 1. DR PROSITE; PS51379; 4FE4S_FER_2; 2. PE 3: Inferred from homology; KW 4Fe-4S {ECO:0000256|PIRSR:PIRSR000159-50}; KW Complete proteome {ECO:0000313|Proteomes:UP000194680}; KW Electron transport {ECO:0000256|PIRNR:PIRNR000159}; KW Iron {ECO:0000256|PIRSR:PIRSR000159-50}; KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR000159-50}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR000159-50}; KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR000159}; KW Pyruvate {ECO:0000313|EMBL:OTO77039.1}; KW Transport {ECO:0000256|PIRNR:PIRNR000159}. FT DOMAIN 687 716 4Fe-4S ferredoxin-type. FT {ECO:0000259|PROSITE:PS51379}. FT DOMAIN 741 772 4Fe-4S ferredoxin-type. FT {ECO:0000259|PROSITE:PS51379}. FT METAL 696 696 Iron-sulfur 1 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 699 699 Iron-sulfur 1 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 702 702 Iron-sulfur 1 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 706 706 Iron-sulfur 2 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 750 750 Iron-sulfur 2 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 753 753 Iron-sulfur 2 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 756 756 Iron-sulfur 2 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 760 760 Iron-sulfur 1 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 816 816 Iron-sulfur 3 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 819 819 Iron-sulfur 3 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 844 844 Iron-sulfur 3 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. FT METAL 1076 1076 Iron-sulfur 3 (4Fe-4S). FT {ECO:0000256|PIRSR:PIRSR000159-50}. SQ SEQUENCE 1177 AA; 130154 MW; 0B448B39A8C50364 CRC64; MMKTMKTMDG NTAAAHISYA FTEVAGIYPI TPSSTMAEVV DEWAEQGRKN IFNETVKVVE MQSEAGAAGT VHGSLKTGVL TTTYTASQGL LLMIPNMFKI AGELLPTVFH VAARAVSTNA LSIFGDHSDV MAARQTGFCM LAEGGVQEVM DLSAVAHLAS IEGKLPFMNF FDGFRTSHEL QKIEVLDYDD LKAMVNWEAL EEFRKLGMNP NRPNVSGTAQ NPDIHFQQRE TVNRYYDEMP AIVQKYMQKI NDLRGTNYDL TNYYGDPEAT EVIIAMGSAS PTIQQTVDYL NANGRKVGFI DIHLYRPFPA ENLLEKLPAT VEKVAVLDRT KEPGSDGEPL LLDVQSVLYR HTNRPIVIGG RYGIASKDVT PDQIVAVFDH LLVPINEMKQ RFTIGINDDV TNLSLPMGEV LDLTPTSTFQ AKFWGLGSDG TVGANKQAIK IIGNNTDYYA QAYFSYDSKK SGGLTNSHLR FGKEPILSIY NVEAADFVGC HNSAYIHQYD LVKGLKDGGT FLLNTVWTEE KVKQLLPSRI KRYLAEHNIN FYIINAMEIA QKTGLGGRIN QVMSTAFFEL TDILDHETYL PLLKAEVDAA YGKKSRTIVE RNWQAIDATV AELKKVEIPA EWAEIEVKPL FNKEDQEKPA YIRNILEPIN RQEGNALSVN DLIENGMLGG VMPSGTAAYE KRGIALEVPE WIPEACTMCN ECAFVCPHAA IRPFLADEEE MEAAPEGFIT RDMRGKDGFN YRIQVSLEDC TGCGLCVEAC PAKEKALVMK SYEDQKDQAV NWAFAMTLRQ KANPAKKFSV KGSQFEQPLL EFSGACAGCG ETPYVKLLTQ LFGDRMMVAN TTGCSSIWGG SAPATPYTVN EDGRGPAWSN SLFEDNAEYG LGMHMANQTK RNRLANKVMK ALENGVGSED TRAQLQGWMN HMLDGEGSQQ RAAKLRAVLG EETDEDLRSI LADHEMFAKP SQWIIGGDGW AYDIGFSGID HVLASGEDVN ILVMDNEVYA NTGGQMSKAT PSAAIAKFAA GGKKTKKKDL GMMAMTYRDV YVAQVAIEAN PTQALKAIQE AEAYPGPSLI IGYTPCINHG IRGGMSQSIE ESKEAVESGY WPLYRYDPVR AQKGKDPMRI DFKKADFSKM NAFLEGQTRF SALHNIKKDD ELVEEMLTQT VDDMEERAKS YTELTNK //