ID A0A223CZR4_9BACL Unreviewed; 160 AA. AC A0A223CZR4; DT 25-OCT-2017, integrated into UniProtKB/TrEMBL. DT 25-OCT-2017, sequence version 1. DT 03-MAY-2023, entry version 18. DE RecName: Full=Inorganic pyrophosphatase {ECO:0000256|HAMAP-Rule:MF_00209}; DE EC=3.6.1.1 {ECO:0000256|HAMAP-Rule:MF_00209}; DE AltName: Full=Pyrophosphate phospho-hydrolase {ECO:0000256|HAMAP-Rule:MF_00209}; DE Short=PPase {ECO:0000256|HAMAP-Rule:MF_00209}; GN Name=ppa {ECO:0000256|HAMAP-Rule:MF_00209}; GN ORFNames=CIG75_07465 {ECO:0000313|EMBL:ASS74832.1}; OS Tumebacillus algifaecis. OC Bacteria; Bacillota; Bacilli; Bacillales; Alicyclobacillaceae; OC Tumebacillus. OX NCBI_TaxID=1214604 {ECO:0000313|EMBL:ASS74832.1, ECO:0000313|Proteomes:UP000214688}; RN [1] {ECO:0000313|EMBL:ASS74832.1, ECO:0000313|Proteomes:UP000214688} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=THMBR28 {ECO:0000313|EMBL:ASS74832.1, RC ECO:0000313|Proteomes:UP000214688}; RX PubMed=25858243; DOI=10.1099/ijs.0.000240; RA Wu Y.F., Zhang B., Xing P., Wu Q.L., Liu S.J.; RT "Tumebacillus algifaecis sp. nov., isolated from decomposing algal scum."; RL Int. J. Syst. Evol. Microbiol. 65:2194-2198(2015). CC -!- FUNCTION: Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) CC forming two phosphate ions. {ECO:0000256|HAMAP-Rule:MF_00209}. CC -!- CATALYTIC ACTIVITY: CC Reaction=diphosphate + H2O = H(+) + 2 phosphate; Xref=Rhea:RHEA:24576, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, CC ChEBI:CHEBI:43474; EC=3.6.1.1; Evidence={ECO:0000256|HAMAP- CC Rule:MF_00209}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946, CC ECO:0000256|HAMAP-Rule:MF_00209}; CC -!- SUBUNIT: Homohexamer. {ECO:0000256|HAMAP-Rule:MF_00209}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00209}. CC -!- SIMILARITY: Belongs to the PPase family. {ECO:0000256|HAMAP- CC Rule:MF_00209}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP022657; ASS74832.1; -; Genomic_DNA. DR AlphaFoldDB; A0A223CZR4; -. DR KEGG; tab:CIG75_07465; -. DR OrthoDB; 5187599at2; -. DR Proteomes; UP000214688; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0004427; F:inorganic diphosphate phosphatase activity; IEA:UniProtKB-UniRule. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0006796; P:phosphate-containing compound metabolic process; IEA:InterPro. DR CDD; cd00412; pyrophosphatase; 1. DR Gene3D; 3.90.80.10; Inorganic pyrophosphatase; 1. DR HAMAP; MF_00209; Inorganic_PPase; 1. DR InterPro; IPR008162; Pyrophosphatase. DR InterPro; IPR036649; Pyrophosphatase_sf. DR PANTHER; PTHR10286; INORGANIC PYROPHOSPHATASE; 1. DR PANTHER; PTHR10286:SF0; SOLUBLE INORGANIC PYROPHOSPHATASE 2; 1. DR Pfam; PF00719; Pyrophosphatase; 1. DR SUPFAM; SSF50324; Inorganic pyrophosphatase; 1. DR PROSITE; PS00387; PPASE; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00209}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00209}; KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00209}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP- KW Rule:MF_00209}; Reference proteome {ECO:0000313|Proteomes:UP000214688}. FT BINDING 18 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 32 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 44 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 54 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 59 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 59 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="2" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 91 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" FT BINDING 128 FT /ligand="substrate" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00209" SQ SEQUENCE 160 AA; 18246 MW; C89D20EE4B07604A CRC64; MAMIVNAFIE IPTGSQNKYE YDKENNRFML DRVLFSPMHY PTEYGYIDNT LAEDGDPLDI LVVTSFPTFP GCVIESRVVG VLIMTDDKGK DEKLLAVPTQ DPRFANVHTL DDVAPHVLKE ISHFFQVYKD LENKQVQIDG WEGVETAQRL LDESTARYAK //