ID A0A223CYH0_9BACL Unreviewed; 745 AA. AC A0A223CYH0; DT 25-OCT-2017, integrated into UniProtKB/TrEMBL. DT 25-OCT-2017, sequence version 1. DT 13-FEB-2019, entry version 7. DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053}; DE EC=3.6.4.12 {ECO:0000256|RuleBase:RU364053}; GN Name=pcrA {ECO:0000313|EMBL:ASS74173.1}; GN ORFNames=CIG75_03680 {ECO:0000313|EMBL:ASS74173.1}; OS Tumebacillus algifaecis. OC Bacteria; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; OC Tumebacillus. OX NCBI_TaxID=1214604 {ECO:0000313|EMBL:ASS74173.1}; RN [1] {ECO:0000313|EMBL:ASS74173.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=THMBR28 {ECO:0000313|EMBL:ASS74173.1}; RX PubMed=25858243; DOI=10.1099/ijs.0.000240; RA Wu Y.F., Zhang B., Xing P., Wu Q.L., Liu S.J.; RT "Tumebacillus algifaecis sp. nov., isolated from decomposing algal RT scum."; RL Int. J. Syst. Evol. Microbiol. 65:2194-2198(2015). RN [2] {ECO:0000313|EMBL:ASS74173.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=THMBR28 {ECO:0000313|EMBL:ASS74173.1}; RA de Groot N.N.; RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC Evidence={ECO:0000256|RuleBase:RU364053, CC ECO:0000256|SAAS:SAAS01121273}; CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily. CC {ECO:0000256|RuleBase:RU364053, ECO:0000256|SAAS:SAAS00709641}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP022657; ASS74173.1; -; Genomic_DNA. DR KEGG; tab:CIG75_03680; -. DR KO; K03657; -. DR BioCyc; GCF_002243515:CIG75_RS03680-MONOMER; -. DR GO; GO:0005737; C:cytoplasm; IEA:InterPro. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro. DR Gene3D; 1.10.10.160; -; 1. DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA. DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf. DR InterPro; IPR014017; DNA_helicase_UvrD-like_C. DR InterPro; IPR000212; DNA_helicase_UvrD/REP. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR014016; UvrD-like_ATP-bd. DR InterPro; IPR034739; UvrD/AddA_N. DR PANTHER; PTHR11070; PTHR11070; 1. DR Pfam; PF00580; UvrD-helicase; 1. DR Pfam; PF13361; UvrD_C; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR01073; pcrA; 1. DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1. DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|RuleBase:RU364053, KW ECO:0000256|SAAS:SAAS00745276}; KW DNA-binding {ECO:0000256|RuleBase:RU364053, KW ECO:0000256|SAAS:SAAS00745286}; KW Helicase {ECO:0000256|RuleBase:RU364053, KW ECO:0000256|SAAS:SAAS00938801, ECO:0000313|EMBL:ASS74173.1}; KW Hydrolase {ECO:0000256|RuleBase:RU364053, KW ECO:0000256|SAAS:SAAS00745169}; KW Nucleotide-binding {ECO:0000256|RuleBase:RU364053, KW ECO:0000256|SAAS:SAAS00745105}. FT DOMAIN 12 293 UvrD-like helicase ATP-binding. FT {ECO:0000259|PROSITE:PS51198}. FT DOMAIN 294 572 UvrD-like helicase C-terminal. FT {ECO:0000259|PROSITE:PS51217}. SQ SEQUENCE 745 AA; 84268 MW; 573E561B2BC5EE46 CRC64; MFSIQGGEKM LENLNPQQKE AVLHSAGPLL IIAGAGSGKT SVLTRRIAYL IEERRVAPWQ ILAITFTNKA AREMRERVEN LIGPTAHDIW ISTFHSMCVR VLRRDADKLG YTNSFTILDA GDQLTAVKQC LKELNLDPKK FDPRGLLATI SNAKNDLLTH ERFASTVRDG DVFGGVAAQV YEAYQKKLKS NNALDFDDLI MKTVYLFQHH EDVLSLYQNR LHYIHVDEYQ DTNKAQYKLV QMLAKKKRNI CVVGDSDQSI YAWRGADITN ILNFEKDYPE TTVIKLEQNY RSTGRILEAA NHVIANNRER KEKNLWSTKA EGELVKLYKA YDEHSEAQFV IQQTLEHLKG GGRHSDIAVL YRTNAQSRVI EEALLKSTVP YTIYGGVKFY ERKEIKDILA YLRLIANLND DLSLTRVINV PKRGIGDTTV GKILDYAATH GISAFQALME IENVGIRGKA NGAIKEFVLL LRNFATQMPY LSVTDLAEEV MKLTGYRKEL ELEKTLEADA RLENLDEFLS VTAEFDNKHE MTEENRLVEF LSDVALIADS EQSEGETGPE SIKLMTLHAA KGLEFPVVFL VGLEEGMFPS NRTLTQGDER DVEEERRLMY VGITRAEEKL FISHAGSRMM YGQIKANPPS RFIREIPPSL LEEVGIIRRQ PAQERVRSDI PGLQNGTRVP SGFGADPNLT WEVGEWVAHR KWGLGEIVKS EGVGDNLELY IQFEDLAIGM KKLLARFAPI VKVED //