ID A0A210PH48_MIZYE Unreviewed; 585 AA. AC A0A210PH48; DT 27-SEP-2017, integrated into UniProtKB/TrEMBL. DT 27-SEP-2017, sequence version 1. DT 22-FEB-2023, entry version 20. DE RecName: Full=O-acyltransferase {ECO:0000256|PIRNR:PIRNR000439}; GN ORFNames=KP79_PYT13687 {ECO:0000313|EMBL:OWF35812.1}; OS Mizuhopecten yessoensis (Japanese scallop) (Patinopecten yessoensis). OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia; OC Autobranchia; Pteriomorphia; Pectinida; Pectinoidea; Pectinidae; OC Mizuhopecten. OX NCBI_TaxID=6573 {ECO:0000313|EMBL:OWF35812.1, ECO:0000313|Proteomes:UP000242188}; RN [1] {ECO:0000313|Proteomes:UP000242188} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=PY_sf001 {ECO:0000313|Proteomes:UP000242188}; RX PubMed=28812685; DOI=10.1038/s41559-017-0120; RA Wang S., Zhang J., Jiao W., Li J., Xun X., Sun Y., Guo X., Huan P., RA Dong B., Zhang L., Hu X., Sun X., Wang J., Zhao C., Wang Y., Wang D., RA Huang X., Wang R., Lv J., Li Y., Zhang Z., Liu B., Lu W., Hui Y., Liang J., RA Zhou Z., Hou R., Li X., Liu Y., Li H., Ning X., Lin Y., Zhao L., Xing Q., RA Dou J., Li Y., Mao J., Guo H., Dou H., Li T., Mu C., Jiang W., Fu Q., RA Fu X., Miao Y., Liu J., Yu Q., Li R., Liao H., Li X., Kong Y., Jiang Z., RA Chourrout D., Li R., Bao Z.; RT "Scallop genome provides insights into evolution of bilaterian karyotype RT and development."; RL Nat. Ecol. Evol. 1:120-120(2017). CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoate + 1,2-di-(9Z-octadecenoyl)-glycerol + H(+) CC = 1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O; Xref=Rhea:RHEA:38379, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30823, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:53753; CC Evidence={ECO:0000256|ARBA:ARBA00000645}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38380; CC Evidence={ECO:0000256|ARBA:ARBA00000645}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1,2-di-(9Z-octadecenoyl)-sn-glycerol = CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38219, CC ChEBI:CHEBI:52333, ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57387; Evidence={ECO:0000256|ARBA:ARBA00000389}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38220; CC Evidence={ECO:0000256|ARBA:ARBA00000389}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1,3-di-(9Z-octadecenoyl)-glycerol = CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38435, CC ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75735; Evidence={ECO:0000256|ARBA:ARBA00000883}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38436; CC Evidence={ECO:0000256|ARBA:ARBA00000883}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di- CC (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:37915, CC ChEBI:CHEBI:52323, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75342; Evidence={ECO:0000256|ARBA:ARBA00001009}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37916; CC Evidence={ECO:0000256|ARBA:ARBA00001009}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-glycerol = 1-O- CC (9Z-octadecenyl)-mono-(9Z-octadecenoyl)-glycerol + CoA; CC Xref=Rhea:RHEA:55340, ChEBI:CHEBI:34116, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57387, ChEBI:CHEBI:138734; CC Evidence={ECO:0000256|ARBA:ARBA00000304}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55341; CC Evidence={ECO:0000256|ARBA:ARBA00000304}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-O-(9Z-octadecenyl)-mono-(9Z- CC octadecenoyl)-glycerol = 1-O-(9Z-octadecenyl)-2,3-di-(9Z- CC octadecenoyl)glycerol + CoA; Xref=Rhea:RHEA:55344, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57387, ChEBI:CHEBI:138734, ChEBI:CHEBI:138735; CC Evidence={ECO:0000256|ARBA:ARBA00000605}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55345; CC Evidence={ECO:0000256|ARBA:ARBA00000605}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoyl-2-(5Z,8Z,11Z,14Z- CC eicosatetraenoyl)-sn-glycerol = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z)- CC eicosatetraenoyl-3-(9Z)-octadecenoyl-sn-glycerol + CoA; CC Xref=Rhea:RHEA:38307, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75728, ChEBI:CHEBI:75729; CC Evidence={ECO:0000256|ARBA:ARBA00001100}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38308; CC Evidence={ECO:0000256|ARBA:ARBA00001100}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 2,3-di-(9Z)-octadecenoyl-sn-glycerol = CC 1,2,3-tri-(9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38439, CC ChEBI:CHEBI:53753, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:75824; Evidence={ECO:0000256|ARBA:ARBA00001338}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38440; CC Evidence={ECO:0000256|ARBA:ARBA00001338}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di- CC (9Z-octadecenoyl)-sn-glycerol + CoA; Xref=Rhea:RHEA:37911, CC ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, CC ChEBI:CHEBI:73990; Evidence={ECO:0000256|ARBA:ARBA00001793}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37912; CC Evidence={ECO:0000256|ARBA:ARBA00001793}; CC -!- CATALYTIC ACTIVITY: CC Reaction=1,2-di-(9Z-octadecenoyl)-sn-glycerol + hexadecanoyl-CoA = 1,2- CC di-(9Z)-octadecenoyl-3-hexadecanoyl-sn-glycerol + CoA; CC Xref=Rhea:RHEA:38163, ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57379, ChEBI:CHEBI:75583; CC Evidence={ECO:0000256|ARBA:ARBA00001349}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38164; CC Evidence={ECO:0000256|ARBA:ARBA00001349}; CC -!- CATALYTIC ACTIVITY: CC Reaction=13-cis-retinol + hexadecanoyl-CoA = 13-cis-retinyl CC hexadecanoate + CoA; Xref=Rhea:RHEA:55296, ChEBI:CHEBI:45479, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:138722; CC Evidence={ECO:0000256|ARBA:ARBA00000895}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55297; CC Evidence={ECO:0000256|ARBA:ARBA00000895}; CC -!- CATALYTIC ACTIVITY: CC Reaction=2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1- CC hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA; CC Xref=Rhea:RHEA:38071, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75466; CC Evidence={ECO:0000256|ARBA:ARBA00001313}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38072; CC Evidence={ECO:0000256|ARBA:ARBA00001313}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20; CC Evidence={ECO:0000256|ARBA:ARBA00001150}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10869; CC Evidence={ECO:0000256|ARBA:ARBA00001150}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinol + an acyl-CoA = an all-trans-retinyl ester + CC CoA; Xref=Rhea:RHEA:11488, ChEBI:CHEBI:17336, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:58342, ChEBI:CHEBI:63410; EC=2.3.1.76; CC Evidence={ECO:0000256|ARBA:ARBA00000633}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11489; CC Evidence={ECO:0000256|ARBA:ARBA00000633}; CC -!- CATALYTIC ACTIVITY: CC Reaction=all-trans-retinol + hexadecanoyl-CoA = all-trans-retinyl CC hexadecanoate + CoA; Xref=Rhea:RHEA:38175, ChEBI:CHEBI:17336, CC ChEBI:CHEBI:17616, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379; CC Evidence={ECO:0000256|ARBA:ARBA00001764}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38176; CC Evidence={ECO:0000256|ARBA:ARBA00001764}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexadecan-1-ol + hexadecanoyl-CoA = CoA + hexadecanyl CC hexadecanoate; Xref=Rhea:RHEA:38167, ChEBI:CHEBI:16125, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75584; CC Evidence={ECO:0000256|ARBA:ARBA00001796}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38168; CC Evidence={ECO:0000256|ARBA:ARBA00001796}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexadecane-1,2-diol + 2 hexadecanoyl-CoA = 1,2-O,O- CC dihexadecanoyl-1,2-hexadecanediol + 2 CoA; Xref=Rhea:RHEA:38211, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, CC ChEBI:CHEBI:75608; Evidence={ECO:0000256|ARBA:ARBA00001118}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38212; CC Evidence={ECO:0000256|ARBA:ARBA00001118}; CC -!- CATALYTIC ACTIVITY: CC Reaction=hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl CC hexadecanoate + CoA; Xref=Rhea:RHEA:38171, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, ChEBI:CHEBI:75587; CC Evidence={ECO:0000256|ARBA:ARBA00000174}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38172; CC Evidence={ECO:0000256|ARBA:ARBA00000174}; CC -!- PATHWAY: Lipid metabolism; glycerolipid metabolism. CC {ECO:0000256|ARBA:ARBA00005175}. CC -!- SUBUNIT: Homodimer or homotetramer; both forms have similar enzymatic CC activities. {ECO:0000256|ARBA:ARBA00023610}. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000256|ARBA:ARBA00004477, ECO:0000256|PIRNR:PIRNR000439}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004477, CC ECO:0000256|PIRNR:PIRNR000439}. Membrane CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family. CC Sterol o-acyltransferase subfamily. {ECO:0000256|ARBA:ARBA00009010, CC ECO:0000256|PIRNR:PIRNR000439}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:OWF35812.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NEDP02076706; OWF35812.1; -; Genomic_DNA. DR AlphaFoldDB; A0A210PH48; -. DR STRING; 6573.A0A210PH48; -. DR EnsemblMetazoa; XM_021486110.1; XP_021341785.1; LOC110442476. DR OrthoDB; 9612at2759; -. DR UniPathway; UPA00230; -. DR Proteomes; UP000242188; Unassembled WGS sequence. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0050252; F:retinol O-fatty-acyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:InterPro. DR InterPro; IPR027251; Diacylglycerol_acylTrfase1. DR InterPro; IPR004299; MBOAT_fam. DR InterPro; IPR014371; Oat_ACAT_DAG_ARE. DR PANTHER; PTHR10408:SF7; DIACYLGLYCEROL O-ACYLTRANSFERASE 1; 1. DR PANTHER; PTHR10408; STEROL O-ACYLTRANSFERASE; 1. DR Pfam; PF03062; MBOAT; 1. DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1. DR PIRSF; PIRSF500231; Oat_dag; 1. PE 3: Inferred from homology; KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315, KW ECO:0000256|PIRNR:PIRNR000439}; KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824, KW ECO:0000256|PIRNR:PIRNR000439}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR000439}; KW Reference proteome {ECO:0000313|Proteomes:UP000242188}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000439}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}. FT TRANSMEM 211..233 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 245..268 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 274..295 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 374..393 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 424..445 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 494..512 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 518..536 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 548..571 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT REGION 84..113 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 93..113 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 507 FT /evidence="ECO:0000256|PIRSR:PIRSR000439-1" SQ SEQUENCE 585 AA; 67686 MW; 98050F213E2AEF44 CRC64; MLIEGDLGDT VIDEASVPMA THPEDFKVSR KTCFMESEGK ITNHNSEKEN YEKTEKLFSK NDSLFESEND EETDCCNKCK LNQRRNRPRN HSSDSAYSDI STSSEQSRNS KGTLYSPDKF RRFMPIWLSD ILDEPEYTMD CKDKTGKDRI HRHADSLFST ASGFTNYRGL LNLCILLLVL ANTRLFLENI LKYGILIDPV KVIHVFLSQP YSWPNICLVF SAGIFTLVAY YMECLLAKGY VSERFGFLVH AINLSLLLII PAAVILYLHP FPAFSSVTCG ICTISFLKLV SYASVNKWCR QRKGGDTKKV RRSRSISESP KSSANGQVAA IITTYPDNLT HSDLWYFMLA PTLCYELNFP RSLRIRKRFL IKRCLEMLFL SQLMLGLIQQ WIVPTVQNSM KPLQDMEVSR VLERILKLAV PNHLIWLIFF YWGFHSCLNV SAELLKFGDR EFYKDWWNAE SVTEFWQTWN VPVHRWCVRH LYKPLVKRGY SKQLASVLVF AMSAFFHEYL VSVPLKMFRV WAFFAMLGQV PLAQFVKKYI HGKYANMVVW LSLILGQPIA IFCYVHDYYM INFVPVAVAN QTVTL //