ID A0A1Z8TE16_9DELT Unreviewed; 245 AA. AC A0A1Z8TE16; DT 22-NOV-2017, integrated into UniProtKB/TrEMBL. DT 22-NOV-2017, sequence version 1. DT 16-JAN-2019, entry version 10. DE RecName: Full=tRNA pseudouridine synthase A {ECO:0000256|HAMAP-Rule:MF_00171}; DE EC=5.4.99.12 {ECO:0000256|HAMAP-Rule:MF_00171}; DE AltName: Full=tRNA pseudouridine(38-40) synthase {ECO:0000256|HAMAP-Rule:MF_00171}; DE AltName: Full=tRNA pseudouridylate synthase I {ECO:0000256|HAMAP-Rule:MF_00171}; DE AltName: Full=tRNA-uridine isomerase I {ECO:0000256|HAMAP-Rule:MF_00171}; GN Name=truA {ECO:0000256|HAMAP-Rule:MF_00171}; GN ORFNames=CBC18_03600 {ECO:0000313|EMBL:OUU32916.1}; OS Deltaproteobacteria bacterium TMED58. OC Bacteria; Proteobacteria; Deltaproteobacteria. OX NCBI_TaxID=1986674 {ECO:0000313|EMBL:OUU32916.1}; RN [1] {ECO:0000313|EMBL:OUU32916.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=TMED58 {ECO:0000313|EMBL:OUU32916.1}; RA Tully B.J., Sachdeva R., Graham E.D., Heidelberg J.F.; RT "290 Metagenome-assembled Genomes from the Mediterranean Sea: a RT resource for marine microbiology."; RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in CC the anticodon stem and loop of transfer RNAs. {ECO:0000256|HAMAP- CC Rule:MF_00171, ECO:0000256|SAAS:SAAS00023495}. CC -!- CATALYTIC ACTIVITY: CC Reaction=tRNA uridine(38-40) = tRNA pseudouridine(38-40); CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA- CC COMP:10087, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00171, CC ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS01119083}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00171}. CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA CC family. {ECO:0000256|HAMAP-Rule:MF_00171, CC ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00543779}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation CC of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00171}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:OUU32916.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NHDL01000020; OUU32916.1; -; Genomic_DNA. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC. DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.70.660; -; 1. DR HAMAP; MF_00171; TruA; 1. DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf. DR InterPro; IPR001406; PsdUridine_synth_TruA. DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom. DR InterPro; IPR020095; PsdUridine_synth_TruA_C. DR PANTHER; PTHR11142; PTHR11142; 1. DR Pfam; PF01416; PseudoU_synth_1; 2. DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1. DR SUPFAM; SSF55120; SSF55120; 1. DR TIGRFAMs; TIGR00071; hisT_truA; 1. PE 3: Inferred from homology; KW Isomerase {ECO:0000256|HAMAP-Rule:MF_00171, KW ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00023490}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_00171, KW ECO:0000256|RuleBase:RU003792, ECO:0000256|SAAS:SAAS00023481}. FT DOMAIN 8 102 PseudoU_synth_1. {ECO:0000259|Pfam: FT PF01416}. FT DOMAIN 145 245 PseudoU_synth_1. {ECO:0000259|Pfam: FT PF01416}. FT ACT_SITE 51 51 Nucleophile. {ECO:0000256|HAMAP-Rule: FT MF_00171, ECO:0000256|PIRSR:PIRSR001430- FT 1}. FT BINDING 111 111 Substrate. {ECO:0000256|HAMAP-Rule: FT MF_00171, ECO:0000256|PIRSR:PIRSR001430- FT 2}. SQ SEQUENCE 245 AA; 28542 MW; F47449F850636EB1 CRC64; MNIKMHIEYI GTEFCGWQKQ AKNKTVQNEI EKKLELIYKT KIILLGSGRT DSGVHGLNQV ANFHINNITN FNPMALKNKL NSLLENNIRI NHCIKVDDNF HAQHSCKKKT YLYKIKIMEE CNVFEIDRFL YIKKDLNIKK LRELAKLFVG EHNFINFCKT GYSGKDTIRK IYSFTIIKRK NFVELKITGN GFLRGMVRLI IGALINYEKN LISKNEVIRA IKNKSKLKIN LSVPACGLYL YDVKY //