ID   A0A1Y4MS17_9FIRM        Unreviewed;       534 AA.
AC   A0A1Y4MS17;
DT   30-AUG-2017, integrated into UniProtKB/TrEMBL.
DT   30-AUG-2017, sequence version 1.
DT   27-NOV-2024, entry version 23.
DE   RecName: Full=Citramalate synthase {ECO:0000256|NCBIfam:TIGR00977};
DE            EC=2.3.3.21 {ECO:0000256|NCBIfam:TIGR00977};
GN   ORFNames=B5F11_03000 {ECO:0000313|EMBL:OUP71050.1};
OS   Anaerotruncus colihominis.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Anaerotruncus.
OX   NCBI_TaxID=169435 {ECO:0000313|EMBL:OUP71050.1, ECO:0000313|Proteomes:UP000196386};
RN   [1] {ECO:0000313|Proteomes:UP000196386}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=An175 {ECO:0000313|Proteomes:UP000196386};
RA   Medvecky M., Cejkova D., Polansky O., Karasova D., Kubasova T., Cizek A.,
RA   Rychlik I.;
RT   "Function of individual gut microbiota members based on whole genome
RT   sequencing of pure cultures obtained from chicken caecum.";
RL   Submitted (APR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pyruvate + acetyl-CoA + H2O = (3R)-citramalate + CoA + H(+);
CC         Xref=Rhea:RHEA:19045, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30934, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.21;
CC         Evidence={ECO:0000256|ARBA:ARBA00034270};
CC   -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-
CC       oxobutanoate from pyruvate: step 1/3. {ECO:0000256|ARBA:ARBA00004743}.
CC   -!- SIMILARITY: Belongs to the alpha-IPM synthase/homocitrate synthase
CC       family. {ECO:0000256|ARBA:ARBA00006154, ECO:0000256|RuleBase:RU003523}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OUP71050.1}.
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DR   EMBL; NFKP01000002; OUP71050.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1Y4MS17; -.
DR   UniPathway; UPA00047; UER00066.
DR   Proteomes; UP000196386; Unassembled WGS sequence.
DR   GO; GO:0043714; F:(R)-citramalate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003852; F:2-isopropylmalate synthase activity; IEA:InterPro.
DR   GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009098; P:L-leucine biosynthetic process; IEA:InterPro.
DR   CDD; cd07941; DRE_TIM_LeuA3; 1.
DR   Gene3D; 1.10.238.260; -; 1.
DR   Gene3D; 3.30.160.270; -; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013709; 2-isopropylmalate_synth_dimer.
DR   InterPro; IPR002034; AIPM/Hcit_synth_CS.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005675; Citramal_synthase.
DR   InterPro; IPR054691; LeuA/HCS_post-cat.
DR   InterPro; IPR036230; LeuA_allosteric_dom_sf.
DR   InterPro; IPR000891; PYR_CT.
DR   NCBIfam; TIGR00977; citramal_synth; 1.
DR   PANTHER; PTHR43538:SF1; (R)-CITRAMALATE SYNTHASE; 1.
DR   PANTHER; PTHR43538; ALPHA-IPM SYNTHASE/HOMOCITRATE SYNTHASE; 1.
DR   Pfam; PF22617; HCS_D2; 1.
DR   Pfam; PF00682; HMGL-like; 1.
DR   Pfam; PF08502; LeuA_dimer; 1.
DR   SMART; SM00917; LeuA_dimer; 1.
DR   SUPFAM; SSF110921; 2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain; 1.
DR   SUPFAM; SSF51569; Aldolase; 1.
DR   PROSITE; PS00815; AIPM_HOMOCIT_SYNTH_1; 1.
DR   PROSITE; PS00816; AIPM_HOMOCIT_SYNTH_2; 1.
DR   PROSITE; PS50991; PYR_CT; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW   Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304};
KW   Isoleucine biosynthesis {ECO:0000256|ARBA:ARBA00022624};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003523}.
FT   DOMAIN          4..267
FT                   /note="Pyruvate carboxyltransferase"
FT                   /evidence="ECO:0000259|PROSITE:PS50991"
SQ   SEQUENCE   534 AA;  57851 MW;  5557C004789FDEE9 CRC64;
     MEKLEIFDST LRDGAQGEGV SFSVEDKLRI AGALDRAGVG LIEAGNPFSN PKDAEFFRRA
     NTELRLKHAR LVAFGSTRRR GVNVRKDQNC AALLASGAPC AAVFGKGSAW QVREVLCATP
     EENLEMIFDT VRWLTDAGME TFFDAEHFFD GYKDNAEYAI RTLRAAAEAG ARRLVLCDTN
     GGCFPDEIER ITAEANRLFP GMIGIHCHND TGCAVANTIA AVRAGARHVQ GAYTGLGERC
     GNTNLSTVIA DLQLKLGYDC VPEDCIPRMT GAARMISEIA NVSLPHTMPY VGKSAFAHKG
     GMHVDGVKKN TAAFEHVDPS LVGNQRHILL SDVSGRSAMI GKIGEVIPGL SKQSPELDLL
     LGKLKELEYA GYQFEAATAS FEMVVLKELG RFQPFFTVEL FRIIGEQDSA NRHMASAMVK
     VRVGDRYEIT ADEGDGPVHA LDRALRKALE VFYPALSGVH LIDYKVRVMD TGRATAALVR
     VLIESSDGES VWTTVGVSTD IINASLHALA DSIEYKLYRD QVGASKKQGD GCGG
//