ID A0A1Y3DYD9_PLAKN Unreviewed; 900 AA. AC A0A1Y3DYD9; DT 30-AUG-2017, integrated into UniProtKB/TrEMBL. DT 30-AUG-2017, sequence version 1. DT 27-MAR-2024, entry version 21. DE SubName: Full=Putative Exodeoxyribonuclease III {ECO:0000313|EMBL:OTN67797.1}; GN ORFNames=PKNOH_S05372700 {ECO:0000313|EMBL:OTN67797.1}; OS Plasmodium knowlesi. OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; OC Plasmodiidae; Plasmodium; Plasmodium (Plasmodium). OX NCBI_TaxID=5850 {ECO:0000313|EMBL:OTN67797.1, ECO:0000313|Proteomes:UP000195012}; RN [1] {ECO:0000313|EMBL:OTN67797.1, ECO:0000313|Proteomes:UP000195012} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Malayan Strain Pk1 (A+) {ECO:0000313|Proteomes:UP000195012}; RA Lapp S.A., Geraldo J.A., Chien J.-T., Ay F., Pakala S.B., Batugedara G., RA Humphrey J.C., Debarry J.D., Le Roch K.G., Galinski M.R., Kissinger J.C.; RT "PacBio assembly of a Plasmodium knowlesi genome sequence with Hi-C RT correction and manual annotation of the SICAvar gene family."; RL Submitted (MAY-2017) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|PIRSR:PIRSR604808-2}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|PIRSR:PIRSR604808-2}; CC Note=Probably binds two magnesium or manganese ions per subunit. CC {ECO:0000256|PIRSR:PIRSR604808-2}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- SIMILARITY: Belongs to the DNA repair enzymes AP/ExoA family. CC {ECO:0000256|ARBA:ARBA00007092}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:OTN67797.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; NETL01000019; OTN67797.1; -; Genomic_DNA. DR AlphaFoldDB; A0A1Y3DYD9; -. DR VEuPathDB; PlasmoDB:PKNH_1328100; -. DR VEuPathDB; PlasmoDB:PKNOH_S05372700; -. DR eggNOG; KOG1294; Eukaryota. DR Proteomes; UP000195012; Unassembled WGS sequence. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0004518; F:nuclease activity; IEA:InterPro. DR GO; GO:0006281; P:DNA repair; IEA:InterPro. DR Gene3D; 3.60.10.10; Endonuclease/exonuclease/phosphatase; 2. DR InterPro; IPR004808; AP_endonuc_1. DR InterPro; IPR036691; Endo/exonu/phosph_ase_sf. DR InterPro; IPR005135; Endo/exonuclease/phosphatase. DR PANTHER; PTHR22748; AP ENDONUCLEASE; 1. DR PANTHER; PTHR22748:SF4; DNA-(APURINIC OR APYRIMIDINIC SITE) ENDONUCLEASE 2; 1. DR Pfam; PF03372; Exo_endo_phos; 1. DR SUPFAM; SSF56219; DNase I-like; 1. PE 3: Inferred from homology; KW Magnesium {ECO:0000256|PIRSR:PIRSR604808-2}; KW Manganese {ECO:0000256|PIRSR:PIRSR604808-2}; KW Metal-binding {ECO:0000256|PIRSR:PIRSR604808-2}. FT DOMAIN 7..255 FT /note="Endonuclease/exonuclease/phosphatase" FT /evidence="ECO:0000259|Pfam:PF03372" FT REGION 367..480 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 676..726 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 370..392 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 408..454 FT /note="Acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 455..480 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 676..711 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT BINDING 10 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-2" FT BINDING 44 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-2" FT BINDING 240 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-2" FT BINDING 242 FT /ligand="Mg(2+)" FT /ligand_id="ChEBI:CHEBI:18420" FT /ligand_label="1" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-2" FT SITE 242 FT /note="Transition state stabilizer" FT /evidence="ECO:0000256|PIRSR:PIRSR604808-3" SQ SEQUENCE 900 AA; 102319 MW; 8F8810425776B04F CRC64; MESLQIASWN VNGWKKSCEL IKQREVTVAH FLKKLNIDIL CLQETKTNNP TIENESSLLD AYSDEYESYW TCCKRRGGGE KIQRGYSGLA TYVGNKAKII CCCSDPFRNF FFSVDGFSEE DLLVSRCFPR DNSSVSFYAF PHDSGEVTDA LSSGYGEQKC QTPRQIQPPD APLQRVSDMF NEGRVLITIH KEFVLVNVYA PYSGCNYNRL DYKMRFLHAV RSKLLELRIT TGLPIILVGD LNISFRNRDV HYLNNVINLH EVQKDMNKVN IREDLKQRIC KQIPIIIETL SSRDNFIIKK QKGENGESFR LYLNFKGDVK RVGTSFTSME EIFFFFSLDP VFVEDKYAGV YPNDFFFFLR APGGGIQRGD DKGGDNKDGD NKYGDNKYGD NKYGDNKYGG IQRGEDEGGD DEGGDDEGGD DEGGDDEGGD DEGGDDEGGD DEGGDDEGGD DEGGDDEGIV HSGEDPPKRS ETHHGNHVER DMYDSFAKER EKICSHFMKK EYAPIQEYLL RMEEKNVKYN IRRITTHGEC RRILCRYSFR NCIEGKYNVK KANTLYLKHF NDILLSLGTF LSKEDLLKVA NALGYSSSPE CCTNFVKNLI CEDRMVDTFS FFYPSLNGKF TCWDTYKQHR VSNEGSRIDY IFVDFILYER LIRSHSHLYA SPVVMTEELR ALLRREGDAK GEGDAKGEGD AKGEGDTKGE GRDEANNGIV MDDDSSKKGV NSANTDTGDP CEENYLCVNS PQNNCNYANY FNRLRKKRGR NPPEEEWVSE QEEDIYHFQF KLRSYIGFIY TSPRLSDHIA VNCTFMARTQ SGGTKKRKEV PICFSGEPTI PLRSFCSSEK QYVTFFPIYL IDPALFSGYT FEILNPVHAH GGTCPSITMA QPHKKTSKIT QYFSVRRGKP //