ID A0A1W5G6R8_9PRIM Unreviewed; 459 AA. AC A0A1W5G6R8; DT 05-JUL-2017, integrated into UniProtKB/TrEMBL. DT 05-JUL-2017, sequence version 1. DT 02-OCT-2024, entry version 27. DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4 {ECO:0000256|ARBA:ARBA00021006, ECO:0000256|RuleBase:RU003297}; DE EC=7.1.1.2 {ECO:0000256|ARBA:ARBA00012944, ECO:0000256|RuleBase:RU003297}; GN Name=ND4 {ECO:0000313|EMBL:ADP68202.1}; OS Lepilemur betsileo. OG Mitochondrion {ECO:0000313|EMBL:ADP68202.1}. OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lemuriformes; OC Lepilemuridae; Lepilemur. OX NCBI_TaxID=886958 {ECO:0000313|EMBL:ADP68202.1}; RN [1] {ECO:0000313|EMBL:ADP68202.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=FAN6.1 {ECO:0000313|EMBL:ADP68202.1}; RX PubMed=28173059; DOI=10.1093/jhered/esw072; RA Lei R., Frasier C.L., Hawkins M.T., Engberg S.E., Bailey C.A., RA Johnson S.E., McLain A.T., Groves C.P., Perry G.H., Nash S.D., RA Mittermeier R.A., Louis E.E.; RT "Phylogenomic Reconstruction of Sportive Lemurs (genus Lepilemur) Recovered RT from Mitogenomes with Inferences for Madagascar Biogeography."; RL J. Hered. 108:107-119(2017). CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from CC NADH through the respiratory chain, using ubiquinone as an electron CC acceptor. Essential for the catalytic activity and assembly of complex CC I. {ECO:0000256|ARBA:ARBA00024313, ECO:0000256|RuleBase:RU003297}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) + CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA- CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976, CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2; CC Evidence={ECO:0000256|ARBA:ARBA00000766, CC ECO:0000256|RuleBase:RU003297}; CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex CC I) which is composed of 45 different subunits. CC {ECO:0000256|ARBA:ARBA00024376}. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Mitochondrion CC inner membrane {ECO:0000256|ARBA:ARBA00004448}; Multi-pass membrane CC protein {ECO:0000256|ARBA:ARBA00004448}. Mitochondrion membrane CC {ECO:0000256|RuleBase:RU003297}; Multi-pass membrane protein CC {ECO:0000256|RuleBase:RU003297}. CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. CC {ECO:0000256|ARBA:ARBA00009025, ECO:0000256|RuleBase:RU003297}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; HQ171066; ADP68202.1; -; Genomic_DNA. DR AlphaFoldDB; A0A1W5G6R8; -. DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell. DR GO; GO:0045271; C:respiratory chain complex I; IEA:TreeGrafter. DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-UniRule. DR GO; GO:0003954; F:NADH dehydrogenase activity; IEA:TreeGrafter. DR GO; GO:0048039; F:ubiquinone binding; IEA:TreeGrafter. DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro. DR GO; GO:0015990; P:electron transport coupled proton transport; IEA:TreeGrafter. DR InterPro; IPR000260; NADH4_N. DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4. DR InterPro; IPR003918; NADH_UbQ_OxRdtase. DR InterPro; IPR001750; ND/Mrp_mem. DR NCBIfam; TIGR01972; NDH_I_M; 1. DR PANTHER; PTHR43507; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4; 1. DR PANTHER; PTHR43507:SF20; NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4; 1. DR Pfam; PF01059; Oxidored_q5_N; 1. DR Pfam; PF00361; Proton_antipo_M; 1. DR PRINTS; PR01437; NUOXDRDTASE4. PE 3: Inferred from homology; KW Electron transport {ECO:0000256|RuleBase:RU003297}; KW Membrane {ECO:0000256|RuleBase:RU003297}; KW Mitochondrion {ECO:0000256|RuleBase:RU003297, ECO:0000313|EMBL:ADP68202.1}; KW NAD {ECO:0000256|RuleBase:RU003297}; KW Respiratory chain {ECO:0000256|RuleBase:RU003297}; KW Transmembrane {ECO:0000256|RuleBase:RU003297}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|RuleBase:RU003297}; Transport {ECO:0000256|RuleBase:RU003297}; KW Ubiquinone {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 21..42 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 62..82 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 94..110 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 116..133 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 145..167 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 193..212 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 224..244 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 256..277 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 284..303 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 309..329 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 350..368 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT TRANSMEM 388..409 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU003297" FT DOMAIN 1..109 FT /note="NADH:ubiquinone oxidoreductase chain 4 N-terminal" FT /evidence="ECO:0000259|Pfam:PF01059" FT DOMAIN 113..397 FT /note="NADH:quinone oxidoreductase/Mrp antiporter membrane FT subunit" FT /evidence="ECO:0000259|Pfam:PF00361" SQ SEQUENCE 459 AA; 51793 MW; 82D7A865C26FAF19 CRC64; MLKIIVPTAM LLPVIWYSTN PMTWMNITLH SLTISLMSLF LFSQADTNSN NFSPTFFSDP LSSPLLVLTM WLLPLTVVAS QYHLTKEPWE RKKHFLSTLI SLQLLLVMTF ATTELIMFYI LFESTLIPTL VIITRWGNQT ERLNAGLYFL FYTLTGSLPL LVALSYIQSS MGTLNLFMMT YWLQELPNSW SNYLLWTACI MAFMIKMPLY GLHMWLPKAH VEAPIAGSMI LAAILLKLGG YGMMRITMIL NPLTKSMAYP FLMLCLWGMI MTSLICLRQT DLKSLIAYSS VSHMALVIVA ILIQTPWSLM GATTLMIAHG LTSSVLFCLA NSNYERVHSR TMLLARGLQA LLPLMSTWWL LASLSNLALP PSINLIGELL VTMAAFSWSN TTIILTGLNM LVTATYSLYM LTTTQRGKSP YHMQNFSPSL TRENTLMSMH ILPLLLLTLN PKILMGPTY //