ID A0A1S2MZW7_9MICC Unreviewed; 613 AA. AC A0A1S2MZW7; DT 12-APR-2017, integrated into UniProtKB/TrEMBL. DT 12-APR-2017, sequence version 1. DT 19-JAN-2022, entry version 16. DE RecName: Full=Dihydroxy-acid dehydratase {ECO:0000256|HAMAP-Rule:MF_00012}; DE Short=DAD {ECO:0000256|HAMAP-Rule:MF_00012}; DE EC=4.2.1.9 {ECO:0000256|HAMAP-Rule:MF_00012}; GN Name=ilvD {ECO:0000256|HAMAP-Rule:MF_00012}; GN ORFNames=BK826_06145 {ECO:0000313|EMBL:OIJ35919.1}; OS Rothia kristinae. OC Bacteria; Actinobacteria; Micrococcales; Micrococcaceae; Rothia. OX NCBI_TaxID=37923 {ECO:0000313|EMBL:OIJ35919.1, ECO:0000313|Proteomes:UP000179540}; RN [1] {ECO:0000313|EMBL:OIJ35919.1, ECO:0000313|Proteomes:UP000179540} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=LCT-H5 {ECO:0000313|EMBL:OIJ35919.1, RC ECO:0000313|Proteomes:UP000179540}; RA Huang B.; RT "Draft genome sequence of strain LCT isolated from the Shenzhou X RT spacecraft of China."; RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + CC H2O; Xref=Rhea:RHEA:20936, ChEBI:CHEBI:11424, ChEBI:CHEBI:11851, CC ChEBI:CHEBI:15377; Evidence={ECO:0000256|HAMAP-Rule:MF_00012}; CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00012}; CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|HAMAP-Rule:MF_00012}; CC -!- PATHWAY: Amino-acid biosynthesis; L-isoleucine biosynthesis; L- CC isoleucine from 2-oxobutanoate: step 3/4. {ECO:0000256|HAMAP- CC Rule:MF_00012}. CC -!- PATHWAY: Amino-acid biosynthesis; L-valine biosynthesis; L-valine from CC pyruvate: step 3/4. {ECO:0000256|HAMAP-Rule:MF_00012}. CC -!- SIMILARITY: Belongs to the IlvD/Edd family. CC {ECO:0000256|ARBA:ARBA00006486, ECO:0000256|HAMAP-Rule:MF_00012}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:OIJ35919.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MODZ01000006; OIJ35919.1; -; Genomic_DNA. DR RefSeq; WP_075514841.1; NZ_MODZ01000006.1. DR EnsemblBacteria; OIJ35919; OIJ35919; BK826_06145. DR UniPathway; UPA00047; UER00057. DR UniPathway; UPA00049; UER00061. DR Proteomes; UP000179540; Unassembled WGS sequence. DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW. DR GO; GO:0004160; F:dihydroxy-acid dehydratase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-UniRule. DR GO; GO:0009099; P:valine biosynthetic process; IEA:UniProtKB-UniRule. DR Gene3D; 3.50.30.80; -; 1. DR HAMAP; MF_00012; IlvD; 1. DR InterPro; IPR042096; Dihydro-acid_dehy_C. DR InterPro; IPR004404; DihydroxyA_deHydtase. DR InterPro; IPR000581; DiOHA_6PGluconate_deHydtase. DR InterPro; IPR020558; DiOHA_6PGluconate_deHydtase_CS. DR InterPro; IPR037237; IlvD/EDD_N. DR Pfam; PF00920; ILVD_EDD; 1. DR SUPFAM; SSF143975; SSF143975; 1. DR TIGRFAMs; TIGR00110; ilvD; 1. DR PROSITE; PS00886; ILVD_EDD_1; 1. DR PROSITE; PS00887; ILVD_EDD_2; 1. PE 3: Inferred from homology; KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_00012}; KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605, ECO:0000256|HAMAP- KW Rule:MF_00012}; KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304, KW ECO:0000256|HAMAP-Rule:MF_00012}; KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_00012}; KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP- KW Rule:MF_00012}; KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00012}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP- KW Rule:MF_00012}. FT REGION 567..588 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT METAL 122 FT /note="Iron-sulfur (4Fe-4S)" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00012" FT METAL 195 FT /note="Iron-sulfur (4Fe-4S)" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00012" SQ SEQUENCE 613 AA; 65863 MW; 34466D064A2805D3 CRC64; MPALRSRTST HGRNMAGARA LWRATGMKKE DFGKPIVAIA NSFTQFVPGH VHLKNMGALV ASAVEEAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRDVI ADSVEYMVNA HRADALVCIS NCDKITPGML MAAMRLNIPV VFVSGGPMES GAPVEGVLEH RIDLVDAMAY AVDEQVSDAQ LAQIEENACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGT TLATQIARKE LFLKAGRTVV ELAKRYYEQD DESVLPRNVA TKASFRNAMS LDVAMGGSTN TVLHILAAAQ EAEVDFTLKD IDEISRHVPC LCKVAPNSTQ FHIEHVHRAG GIPAILGELD RAGLLEHDGV HTVHADSVDA WLDEWDIRRD TASERARELY LAAPGGVRTT QAFSTANRFE SHDTDAEFGC IRSVEHAYTK DGGLAVLYGN IAEDGAVIKT AGIDEYLFRF IGKAFVVESQ DEAVFEILSK HVQPGDIVVI QYEGPKGGPG MQEMLYPTSY LKGLGLGKEC ALITDGRFSG GTSGISIGHI SPEAASGGAV GLIRTGDEIE IDVTQRLLQV NVSDEELARR REERGPLPWK PTKPRERQVS KALRAYASMV TSADKGAVRV VPE //