ID A0A1R1RXH1_9BACI Unreviewed; 443 AA. AC A0A1R1RXH1; DT 12-APR-2017, integrated into UniProtKB/TrEMBL. DT 12-APR-2017, sequence version 1. DT 25-MAY-2022, entry version 15. DE SubName: Full=NAD-dependent malic enzyme 1 {ECO:0000313|EMBL:OMI30706.1}; GN ORFNames=BTA31_01190 {ECO:0000313|EMBL:OMI30706.1}; OS Bacillus haynesii. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. OX NCBI_TaxID=1925021 {ECO:0000313|EMBL:OMI30706.1, ECO:0000313|Proteomes:UP000187046}; RN [1] {ECO:0000313|EMBL:OMI30706.1, ECO:0000313|Proteomes:UP000187046} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NRRL B-41327 {ECO:0000313|EMBL:OMI30706.1, RC ECO:0000313|Proteomes:UP000187046}; RA Dunlap C.; RT "Bacillus phylogenomics."; RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases. CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|PIRSR:PIRSR000106-3}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|PIRSR:PIRSR000106-3}; CC Note=Divalent metal cations. Prefers magnesium or manganese. CC {ECO:0000256|PIRSR:PIRSR000106-3}; CC -!- COFACTOR: CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|ARBA:ARBA00001936}; CC -!- SIMILARITY: Belongs to the malic enzymes family. CC {ECO:0000256|ARBA:ARBA00008785}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:OMI30706.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; MRBL01000001; OMI30706.1; -; Genomic_DNA. DR RefSeq; WP_076788419.1; NZ_MRBL01000001.1. DR EnsemblBacteria; OMI30706; OMI30706; BTA31_01190. DR Proteomes; UP000187046; Unassembled WGS sequence. DR GO; GO:0004471; F:malate dehydrogenase (decarboxylating) (NAD+) activity; IEA:UniProtKB-EC. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. DR GO; GO:0051287; F:NAD binding; IEA:InterPro. DR GO; GO:0008948; F:oxaloacetate decarboxylase activity; IEA:UniProtKB-EC. DR Gene3D; 3.40.50.10380; -; 1. DR InterPro; IPR002912; ACT_dom. DR InterPro; IPR046346; Aminiacid_DH-like_N_sf. DR InterPro; IPR006140; D-isomer_DH_NAD-bd. DR InterPro; IPR015884; Malic_enzyme_CS. DR InterPro; IPR012301; Malic_N_dom. DR InterPro; IPR037062; Malic_N_dom_sf. DR InterPro; IPR012302; Malic_NAD-bd. DR InterPro; IPR001891; Malic_OxRdtase. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR Pfam; PF02826; 2-Hacid_dh_C; 1. DR Pfam; PF00390; malic; 1. DR Pfam; PF03949; Malic_M; 1. DR PIRSF; PIRSF000106; ME; 1. DR SMART; SM01274; malic; 1. DR SMART; SM00919; Malic_M; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR SUPFAM; SSF53223; SSF53223; 1. DR PROSITE; PS51671; ACT; 1. DR PROSITE; PS00331; MALIC_ENZYMES; 1. PE 3: Inferred from homology; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRSR:PIRSR000106-3}. FT DOMAIN 9..84 FT /note="ACT" FT /evidence="ECO:0000259|PROSITE:PS51671" FT ACT_SITE 112 FT /note="Proton donor" FT /evidence="ECO:0000256|PIRSR:PIRSR000106-1" FT ACT_SITE 167 FT /note="Proton acceptor" FT /evidence="ECO:0000256|PIRSR:PIRSR000106-1" FT METAL 209 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|PIRSR:PIRSR000106-3" FT METAL 210 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|PIRSR:PIRSR000106-3" FT METAL 235 FT /note="Divalent metal cation" FT /evidence="ECO:0000256|PIRSR:PIRSR000106-3" SQ SEQUENCE 443 AA; 46714 MW; 780A56C4D4A32CE8 CRC64; MNGNNIIRNL MLEIPSAPGN LGKVTTAIGL AGGDIGEVET IKVGPNYTMR NITVQVEHEE QLTEILEVIR NLKEGIHLHT VSDDVLAAHE GGKIRMKSKM PIRSLADLRR VYTPGVANVC ELIKEEPQKA NIYTGIGNSV AIVTDGTAIL GLGDIGPVAG MPVMEGKAAL FDQLAAISGV PILLDTNDPD EIVRTVKNIA PGFSGILLED IGSPHCFEIE ERLKNDLNIP VMHDDQHGTA VVTLAAAISA AKSAGVDLKQ AHVGQIGLGA AGVAICRMFM AYGVKRVVGT DKSLEAMARL EKYGGHAAES IEELMESCDI IVATTGVQGL INKKWVREGQ VILALSNPKP EIEPEDALEA GAAYAADGRS VNNVLGFPGI FRGVLNAGAD SISHPMLVAA AEAIASCTKQ GDLVPHPLNP EVHEIVAKAV ERVALSEMGK MNG //