ID STMP4_ARATH Reviewed; 97 AA. AC A0A1P8AQ95; DT 22-FEB-2023, integrated into UniProtKB/Swiss-Prot. DT 12-APR-2017, sequence version 1. DT 28-JUN-2023, entry version 23. DE RecName: Full=Secreted transmembrane peptide 4 {ECO:0000303|PubMed:31001913, ECO:0000303|PubMed:34535661}; DE AltName: Full=Phytocytokine STMP4 {ECO:0000305}; DE AltName: Full=Precursor of secreted transmembrane peptide 4 {ECO:0000305}; DE Flags: Precursor; GN Name=STMP4 {ECO:0000303|PubMed:31001913, ECO:0000303|PubMed:34535661}; GN OrderedLocusNames=At1g65486 {ECO:0000312|Araport:AT1G65486}; GN ORFNames=F5I14 {ECO:0000312|EMBL:AC001229}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., RA Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."; RL Nature 408:816-820(2000). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [3] RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RP INDUCTION BY BIOTIC AND ABIOTIC STRESSES, GENE FAMILY, AND NOMENCLATURE. RC STRAIN=cv. Columbia; RX PubMed=31001913; DOI=10.1111/jipb.12817; RA Yu Z., Xu Y., Zhu L., Zhang L., Liu L., Zhang D., Li D., Wu C., Huang J., RA Yang G., Yan K., Zhang S., Zheng C.; RT "The Brassicaceae-specific secreted peptides, STMPs, function in plant RT growth and pathogen defense."; RL J. Integr. Plant Biol. 62:403-420(2020). RN [4] RP GENE FAMILY. RC STRAIN=cv. Columbia; RX PubMed=34535661; DOI=10.1038/s41467-021-25580-w; RA Hou S., Liu D., Huang S., Luo D., Liu Z., Xiang Q., Wang P., Mu R., Han Z., RA Chen S., Chai J., Shan L., He P.; RT "The Arabidopsis MIK2 receptor elicits immunity by sensing a conserved RT signature from phytocytokines and microbes."; RL Nat. Commun. 12:5494-5494(2021). CC -!- FUNCTION: Brassicaceae-specific phytocytokine (plant endogenous peptide CC released into the apoplast) perceived by MIK2 in a BAK1/SERK3 and SERK4 CC coreceptors-dependent manner, that modulates various physiological and CC antimicrobial processes including growth prevention and reactive oxygen CC species (ROS) response regulation (By similarity). Prevents general CC growth and development (PubMed:31001913). CC {ECO:0000250|UniProtKB:B3H7I1, ECO:0000269|PubMed:31001913}. CC -!- SUBUNIT: Interacts with MIK2 (via extracellular leucine-rich repeat CC domain); this interaction triggers the formation of complex between CC MIK2 and the BAK1/SERK3 and SERK4 coreceptors, and subsequent BAK1 CC activation by phosphorylation. {ECO:0000250|UniProtKB:B3H7I1}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:31001913}. CC Secreted, extracellular space, apoplast {ECO:0000269|PubMed:31001913}. CC Note=The precursor of STMP4 accumulates at the plasma membrane and is CC proteolytically cleaved to release the STMP4 in the apoplasm. CC {ECO:0000269|PubMed:31001913}. CC -!- TISSUE SPECIFICITY: Mostly expressed in leaves and stems, and, to a CC lower extent, in roots, siliques, seeds and flowers. CC {ECO:0000269|PubMed:31001913}. CC -!- INDUCTION: Induced by drought, salt stress, jasmonate (MeJA), ethylene CC (ET) and salicylic acid (SA), mainly in shoots. CC {ECO:0000269|PubMed:31001913}. CC -!- DISRUPTION PHENOTYPE: Slightly increased growth and fresh weight. CC {ECO:0000269|PubMed:31001913}. CC -!- SIMILARITY: Belongs to the serine rich endogenous peptide (SCOOP) CC phytocytokine family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AC001229; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; CP002684; ANM58826.1; -; Genomic_DNA. DR RefSeq; NP_001321237.1; NM_001334209.1. DR AlphaFoldDB; A0A1P8AQ95; -. DR EnsemblPlants; AT1G65486.3; AT1G65486.3; AT1G65486. DR GeneID; 6240283; -. DR Gramene; AT1G65486.3; AT1G65486.3; AT1G65486. DR Araport; AT1G65486; -. DR TAIR; locus:4515102720; AT1G65486. DR InParanoid; A0A1P8AQ95; -. DR OrthoDB; 691471at2759; -. DR Proteomes; UP000006548; Chromosome 1. DR ExpressionAtlas; A0A1P8AQ95; baseline and differential. DR GO; GO:0048046; C:apoplast; IDA:UniProtKB. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0030275; F:LRR domain binding; ISS:UniProtKB. DR GO; GO:0033612; F:receptor serine/threonine kinase binding; ISS:UniProtKB. DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB. DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB. DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB. DR GO; GO:0009651; P:response to salt stress; IEP:UniProtKB. DR GO; GO:0009414; P:response to water deprivation; IEP:UniProtKB. PE 2: Evidence at transcript level; KW Apoplast; Cell membrane; Cleavage on pair of basic residues; Membrane; KW Reference proteome; Secreted; Signal. FT SIGNAL 1..33 FT /evidence="ECO:0000255" FT PROPEP 34..? FT /note="Removed in mature form" FT /evidence="ECO:0000250|UniProtKB:B3H7I1" FT /id="PRO_0000457262" FT PEPTIDE ?..97 FT /note="Secreted transmembrane peptide 4" FT /evidence="ECO:0000250|UniProtKB:B3H7I1" FT /id="PRO_0000457263" FT REGION 75..97 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOTIF 54..67 FT /note="SCOOP motif" FT /evidence="ECO:0000303|PubMed:34535661" FT MOTIF 60..62 FT /note="SxS motif essential for MIK2 binding" FT /evidence="ECO:0000250|UniProtKB:B3H7I1" SQ SEQUENCE 97 AA; 10464 MW; 9051E2BD9F81CB24 CRC64; MTKNMTKKKM GLMSPNIAAF VLPMLLVLFT ISSQVEVVES TGRKLSWAFN GAPIVFTPPS SSCGGSPAAV MASEWMPRRP CRRTRPPGTN IPVSQSP //