ID A0A1M6LPP2_9FIRM Unreviewed; 167 AA. AC A0A1M6LPP2; DT 15-MAR-2017, integrated into UniProtKB/TrEMBL. DT 15-MAR-2017, sequence version 1. DT 16-JAN-2019, entry version 11. DE RecName: Full=Crossover junction endodeoxyribonuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034, ECO:0000256|SAAS:SAAS01093233}; DE EC=3.1.22.4 {ECO:0000256|HAMAP-Rule:MF_00034, ECO:0000256|SAAS:SAAS01093215}; DE AltName: Full=Holliday junction nuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034}; DE AltName: Full=Holliday junction resolvase RuvC {ECO:0000256|HAMAP-Rule:MF_00034}; GN Name=ruvC {ECO:0000256|HAMAP-Rule:MF_00034}; GN ORFNames=SAMN02745227_00598 {ECO:0000313|EMBL:SHJ73166.1}; OS Anaerobranca californiensis DSM 14826. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Proteinivoraceae; OC Anaerobranca. OX NCBI_TaxID=1120989 {ECO:0000313|EMBL:SHJ73166.1, ECO:0000313|Proteomes:UP000243547}; RN [1] {ECO:0000313|EMBL:SHJ73166.1, ECO:0000313|Proteomes:UP000243547} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 14826 {ECO:0000313|EMBL:SHJ73166.1, RC ECO:0000313|Proteomes:UP000243547}; RA Jaros S., Januszkiewicz K., Wedrychowicz H.; RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Nuclease that resolves Holliday junction intermediates CC in genetic recombination. Cleaves the cruciform structure in CC supercoiled DNA by nicking to strands with the same polarity at CC sites symmetrically opposed at the junction in the homologous arms CC and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl CC group. {ECO:0000256|HAMAP-Rule:MF_00034, CC ECO:0000256|SAAS:SAAS01093222}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Endonucleolytic cleavage at a junction such as a CC reciprocal single-stranded crossover between two homologous DNA CC duplexes (Holliday junction).; EC=3.1.22.4; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00034, CC ECO:0000256|SAAS:SAAS01125862}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP- CC Rule:MF_00034}; CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP- CC Rule:MF_00034}; CC -!- SIMILARITY: Belongs to the RuvC family. {ECO:0000256|HAMAP- CC Rule:MF_00034, ECO:0000256|SAAS:SAAS01093228}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FRAI01000005; SHJ73166.1; -; Genomic_DNA. DR RefSeq; WP_072906180.1; NZ_FRAI01000005.1. DR Proteomes; UP000243547; Unassembled WGS sequence. DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:UniProtKB-UniRule. DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule. DR Gene3D; 3.30.420.10; -; 1. DR HAMAP; MF_00034; RuvC; 1. DR InterPro; IPR012337; RNaseH-like_sf. DR InterPro; IPR036397; RNaseH_sf. DR InterPro; IPR020563; X-over_junc_endoDNase_Mg_BS. DR InterPro; IPR002176; X-over_junc_endoDNase_RuvC. DR PANTHER; PTHR30194; PTHR30194; 1. DR Pfam; PF02075; RuvC; 1. DR PRINTS; PR00696; RSOLVASERUVC. DR SUPFAM; SSF53098; SSF53098; 1. DR TIGRFAMs; TIGR00228; ruvC; 1. DR PROSITE; PS01321; RUVC; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000243547}; KW DNA damage {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093219}; KW DNA recombination {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093229}; KW DNA repair {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093231}; KW Endonuclease {ECO:0000313|EMBL:SHJ73166.1}; KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093226}; KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093225}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093227}; KW Nuclease {ECO:0000256|HAMAP-Rule:MF_00034, KW ECO:0000256|SAAS:SAAS01093220}. FT METAL 7 7 Magnesium. {ECO:0000256|HAMAP-Rule: FT MF_00034}. FT METAL 67 67 Magnesium. {ECO:0000256|HAMAP-Rule: FT MF_00034}. FT METAL 140 140 Magnesium. {ECO:0000256|HAMAP-Rule: FT MF_00034}. FT METAL 143 143 Magnesium. {ECO:0000256|HAMAP-Rule: FT MF_00034}. SQ SEQUENCE 167 AA; 18526 MW; DCBA77F85DDA0A86 CRC64; MRIMGIDPGL AIVGFGIIDL DVRKVKVVDY GTIQTDNSLC YTDRLLCVGN SLEKILKIYQ PDVVAIEELF FNKNSKTAFA ISQVRGVIIY TVLKKEIPLY EYTPLQVKQG VVGYGRATKN QVQLMVKNLL NLKEIPKPDD AADGLAVALC HKNFLRSNGI NLQGVVK //