ID A0A1L7P0A7_9ACTN Unreviewed; 6723 AA. AC A0A1L7P0A7; DT 15-MAR-2017, integrated into UniProtKB/TrEMBL. DT 15-MAR-2017, sequence version 1. DT 13-SEP-2023, entry version 29. DE SubName: Full=Modular polyketide synthase {ECO:0000313|EMBL:BAW35610.1}; GN Name=nmd3 {ECO:0000313|EMBL:BAW35610.1}; OS Streptomyces sp. RK95-74. OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales; OC Streptomycetaceae; Streptomyces. OX NCBI_TaxID=1934390 {ECO:0000313|EMBL:BAW35610.1}; RN [1] {ECO:0000313|EMBL:BAW35610.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=RK95-74 {ECO:0000313|EMBL:BAW35610.1}; RA Zhang L., Hashimoto T., Qin B., Hashimoto J., Kozone I., Kawahara I., RA Okada M., Awakawa T., Ito T., Asakawa Y., Ueki M., Takahashi S., Osada H., RA Wakimoto T., Ikeda H., Shin-ya K., Abe I.; RT "Characterization of Giant Modular PKSs Provides Insight into Genetic RT Mechanism for Structural Diversification of Aminopolyol Polyketides."; RL Angew. Chem. Int. Ed. Engl. 56:1740-1745(2017). CC -!- COFACTOR: CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942; CC Evidence={ECO:0000256|ARBA:ARBA00001957}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LC208004; BAW35610.1; -; Genomic_DNA. DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro. DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt. DR CDD; cd08952; KR_1_SDR_x; 2. DR CDD; cd08956; KR_3_FAS_SDR_x; 2. DR CDD; cd00833; PKS; 4. DR Gene3D; 3.30.70.3290; -; 4. DR Gene3D; 3.40.47.10; -; 4. DR Gene3D; 6.10.140.1830; -; 2. DR Gene3D; 1.10.1200.10; ACP-like; 4. DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 4. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 4. DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 2. DR InterPro; IPR001227; Ac_transferase_dom_sf. DR InterPro; IPR036736; ACP-like_sf. DR InterPro; IPR014043; Acyl_transferase. DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase. DR InterPro; IPR018201; Ketoacyl_synth_AS. DR InterPro; IPR014031; Ketoacyl_synth_C. DR InterPro; IPR014030; Ketoacyl_synth_N. DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR032821; PKS_assoc. DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom. DR InterPro; IPR041618; PKS_DE. DR InterPro; IPR042104; PKS_dehydratase_sf. DR InterPro; IPR020807; PKS_DH. DR InterPro; IPR013968; PKS_KR. DR InterPro; IPR020806; PKS_PP-bd. DR InterPro; IPR015083; Polyketide_synth_docking. DR InterPro; IPR009081; PP-bd_ACP. DR InterPro; IPR006162; Ppantetheine_attach_site. DR InterPro; IPR016039; Thiolase-like. DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1. DR PANTHER; PTHR43775:SF51; INACTIVE PHENOLPHTHIOCEROL SYNTHESIS POLYKETIDE SYNTHASE TYPE I PKS1-RELATED; 1. DR Pfam; PF00698; Acyl_transf_1; 4. DR Pfam; PF08990; Docking; 1. DR Pfam; PF16197; KAsynt_C_assoc; 4. DR Pfam; PF00109; ketoacyl-synt; 4. DR Pfam; PF02801; Ketoacyl-synt_C; 4. DR Pfam; PF08659; KR; 4. DR Pfam; PF18369; PKS_DE; 2. DR Pfam; PF00550; PP-binding; 4. DR Pfam; PF14765; PS-DH; 2. DR SMART; SM00827; PKS_AT; 4. DR SMART; SM00826; PKS_DH; 2. DR SMART; SM00822; PKS_KR; 4. DR SMART; SM00825; PKS_KS; 4. DR SMART; SM00823; PKS_PP; 4. DR SMART; SM01294; PKS_PP_betabranch; 4. DR SUPFAM; SSF47336; ACP-like; 4. DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 4. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 8. DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 4. DR SUPFAM; SSF53901; Thiolase-like; 4. DR PROSITE; PS50075; CARRIER; 4. DR PROSITE; PS00606; KS3_1; 4. DR PROSITE; PS52004; KS3_2; 4. DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 4. PE 4: Predicted; KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194}; KW Coiled coil {ECO:0000256|SAM:Coils}; NADP {ECO:0000256|ARBA:ARBA00022857}; KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 34..460 FT /note="Ketosynthase family 3 (KS3)" FT /evidence="ECO:0000259|PROSITE:PS52004" FT DOMAIN 1493..1568 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT DOMAIN 1589..2016 FT /note="Ketosynthase family 3 (KS3)" FT /evidence="ECO:0000259|PROSITE:PS52004" FT DOMAIN 3255..3330 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT DOMAIN 3351..3777 FT /note="Ketosynthase family 3 (KS3)" FT /evidence="ECO:0000259|PROSITE:PS52004" FT DOMAIN 4787..4862 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT DOMAIN 4886..5312 FT /note="Ketosynthase family 3 (KS3)" FT /evidence="ECO:0000259|PROSITE:PS52004" FT DOMAIN 6557..6632 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT REGION 3396..3415 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 5..32 FT /evidence="ECO:0000256|SAM:Coils" SQ SEQUENCE 6723 AA; 701593 MW; 5F47E257C5E5F22E CRC64; MTTPNEKLVD ALRASLKETE RLRRQNQQLT DASREPIAIV GMSCRFPGGV GSPEDLWQLV AGGQDAISGF PADRGWDMEA LYDPDPERAG TFYARGGGFL YDADQFDPGF FGISPREALA MDPQQRLLLE TSWEAFERAG IDPATMRGSR TGVFVGASTQ AYGAGGYDLP EGVEGHLLTG TAASVASGRL SYTLGLEGPA ATIDTACSSS SVALHLAVQA LRQSECSLAL AAGVTVLSSP DVFVEFSRQR GLSADGRCKS FAAAADGTGW SEGVGVLLVE RLSDARRNGH QVLAVVRGSA VNQDGASNGL TAPNGPSQRR VIRQALESAR LTPADVDAVE AHGTGTTLGD PIEAQALLAT YGQDRPEGRP LWLGSLKSNI GHTQNVAGIG GVIKMVMAMR HGVLPQTLHV DEPTPHVDWS AGEVSLLTES RPWPETGRPR RAGVSSFGVS GTNVHTIIEQ APETEPAAEQ TAPTVVAPVL PWVLSGRGGE ATRAQAERLL TFLRNPADGA TPPGVPDIAY SLATTRAAFE HRAVIVAGDR DGFLTRLEAL AADQPVAGLI QGSPVTGKLA FLFTGQGSQR LGMGRELYEA YPVFAEALDA VCAELDQYLE RPLKGVLFGD DAAVLDQTGF TQPALFAVEV ALFRLVEAWG LRPDFLSGHS IGELAAAHVA GVLSLADAAK LVAARGRLMQ DLPAGGAMVA VQASEDEVAR LLTERVSIAA VNGPTSVVIA GDEDAALEIA AGFEQQGRKT KRLTVSHAFH SPRMDGMLEA FREVAQGLSY EAPRIPIVSN LTGELVSAEE IATADFWVRH VREAVRFLDG VRTLEAQGVT TYVELGPDGV LSAMAQDCVT DAEAAVLAPV CRSGRPEAET VNAALAQAYV RGAEVDWAAF FDGTGARRVD LPTYAFQRRR YWLEAPPEAA ESTAPAVNVV EGRFWEAVER GDWEALAGEL RVEGDQPLSA VLPALSSWRR EQQELSTVDG RLYNVVWRPQ ARLRGGSSET AALTGTWLVA VPADRADDAW VAAVTDDLAR RGVRVVRAEL DAADADVELL ADRVALAAAD GPVAGVLSLL GLDERPHPEH PAVPVGIALT GALVEALDQQ DGYASARLWS VTRGAVSTGG ADRLDSLTQA QFWGFGRAVG LGWPERWGGL VDLPETLDGR ALDRLAGVLT GVSGEDQVAI RAAGVFVRRA VRAPRDVASA EPWTPRGTVL VTGGTGALGQ HVARWLARNG AERLVLLSRR GPEAPGAAEL VAELSGTGTV VNVEACDVAD RAALEALVER LAADGAPVRA VVHAAGVSQE PGTDGDLSEL ARVVEAKTAG AIHLDSIFDE SSDPLDAFIL FSSISGIWGS GGQGAYSAGN AFLDALAERR RARGLAATAV AWGPWADGGM AAEGDAEEQL SRRGLRVMAP DLNLTALQRA LSRPEAVVTV ADVDWEVFLS TFTAVRPSPL LAELAPVRDV GGRDSDGRGE GGGESASALA ERLLGLPEAE RKRVLLDLVR THVAGVLGHA DVAEVSAERA FREIGFDSLT AVELRNGLNA ATELRLPATL IYDYPNPVAL ADHLFTELLG GRAAGVDGPA VAGSVATDDE PIAIVAMSCR FPGGVRTPEE LWQLLMSGTD AISGMPTDRG WDLEALYHPD PDQPGTSYAR DGGFLDDAAD FDPGFFGISP REALAMDPQQ RLLLETSWEA FERAGIDPAT VRGSAAGVFV GTNGQDYLSV LLSGEQDGID GHVGTGNAAS VISGRLAYTF GLEGPALTID TACSSSLVAL HTAVQALRAG ECSMALAGGV TVMSTPGAFI EFSRQRGLAQ DGRVKAFAAG ADGTGWGEGV GMLLVERLSD ARRNGHPVLA VVRGTAVNQD GASNGLTAPN GPSQQRVIRA ALANAGLSAT EVDVVEAHGT GTALGDPIEA QALLATYGQE RDADRPLRLG SVKSNLGHTQ AAAGVAGVMK IVLAMRHGVL PRTLHVDEPS PHVDWSAGDI ELLAENQPWP ETGQPRRAGV SSFGFSGTNA HAVLEQAPVA EVESAGPVAG EGEAPADGAL PVVLSGKTPE ALRAQAERLR DHLAGEDGVR LADLGFSLAV GRSALDERAA LVAEDRAGLL AGLEALARGE SAAGLVQGSA TGGRVAFLFT GQGSQRLGMG RELYEAYPVF ADALDEVCAE LDAHLERPLR DVLFGDDAEA LDRTGFTQPA LFAVEVALFR LVEAWGLTPD FLSGHSIGEL AAAHVAGVLS LADAAKLVAA RGRLMQELPA GGAMIAVQAS EDEVAPLLNE RVSIAAVNGP TSVVVAGDED AALEIAAGFE EQGRKTKRLT VSHAFHSPRM DGMLEAFRAV AEGLSYEAPR IPIVSNLTGA VVSAEEITSP DFWVRHVREA VRFLDGVRTL EARGVTTFVE LGPDGVLSAM AQECVTGEDA AFITALRKDR AEAEALTTAV ARAHVRGVAV DWTAFYAGRG ATRIELPTYA FQRQRFWPRV SSLFFGDVAA VGLGEAGHPL LAATAELPEV DGMLFTGRLA LATHPWLAEH TILDTVLLPG TALVELAVRA GDQVGCDHLE ELTLEVPLAL PEQGGVQLRL AVGAADDAGR RSLTLHSRQE GLSAEEPWTR HASGVLSPGG RPEASFELSV WPPADATEID LDGRYEGLRD IGFAYGPVFQ GLRRAWQRDG EIFAEVTLPE DAAEEAGRFG LHPALLDSAL HAVGLEGLGN TDGQGRLPFA WSGVSLYASG AAALRVRLAP AGGDGVRLEI ADTAGVPVAA VEALRLRPVS AEQVRSARTE HHESLFRLEW TELAGVAGTD GGGRWAVLGS DDLVRDAAGV GADVAAYADL TELTAAIESG AAVPRVVLAA PSARGGTDAA GAAHRAATEA LALVRAWLDD ERLADARLAV LTSGALAAEA GEPVTDLAGA TVRGLLRSAQ SENPDRFVLI DVDGRQDWPA ALPAALDSGE PDIAVRQGAL RIPRLARATS GADSRAPEWN PDGTVLITGA GGSLGGLFAR RLVAEHGVRH LLLVSRRGET APGAAELGAE LAAAGAEVRW AACDVADRDA LAEVLAAVPA EHPLTGVVHT AGVLDDGVIG SLTPERLAGV LRPKVDAAWN LHQLTQDLDL TAFVLFSSVA AVFGSPGQGN YAAANAFLDA LAQHRAARGL PGTSLAWGLW AGGGMGDTLD EADIARMRRA GLPPLPVAEG LALFHTALSV GEASLVPMRV DLPGLRTQAA AGAVSPLLRG LVRVPARRAV EAVAGAVESD LTRRLSGLSE PEQERLLLDL VRAQVATVLG YAGAETIGSA RAFKELGFDS LTAVELRNHL NTVTGLRLPA TLVFDYPDPT VLARHLRTEL VGDQVGEDAA AAVPTAAVTD DDAIAIVGMS CRYPGGVGTP EELWQLVMGS VDAVSDFPAD RGWDLDGLRS DDPGRAGTST TGQGGFLHDA AEFDPDFFGI SPREALAMDP QQRLLLETSW EAFERAGIDP GALRGSRTGV FAGVMYHDYL SRLNAVPEGL EGYLGTGSAG SVASGRVAYT FGLEGPAVTI DTACSSSLVA LHLAAQALRN GECSLALAGG VTVMATPETF IDFSRQGGLS TSGRCKSFAA SADGTGWAEG AGMLLVERLS DARRNGHRVL AVVRGSAINQ DGASNGLTAP NGPSQQRVIR EALTSARLTT ADVDVVEAHG TGTKLGDPIE AQALLATYGR ERPEGQPLWL GSLKSNIGHA QAAAGVAGVI KMIMAMRHGV LPRTLHIDEP TPHVDWAAGD VELLTESRPW PETGRPRRAA VSSFGVSGTN AHTILEQPPA VEAAPASDVA LPTVPWVLSG KSEAALRGQA ARLLDHLGTH PDLDATDVGY ALATGRAALE RRAVVLGGDR VALDALARGE SAPGLVEGAV SDGKVAFLFT GQGSQRLGMG RELYDAYPVF AEALDAVCDE LDRHLAQPVK TVLFGQDAEV LDRTAFAQPA LFAVEVALFR LVEAWGVRAD FLSGHSIGEL AAAHVAGVLS LADAAKLVAA RGRLMQELPV GGAMVAVQAS EGEVAPLLSE RVSIAAVNGP TSVVVAGDED AALEIAAGFE AQGRKTKRLT VSHAFHSPRM DGMLEAFRAV AEGLSYDAPR IPIVSNLTGA VVSAEEITSP DFWVRHVREA VRFLDGVCAL EAQGVTTYVE LGPDGVLTAM AQECATGADA AFVTALRKGR AEPESLTGAL AALHTRGVAL DWRAFFSRTG AARVELPTYA FQRQRFWVED PEEVREAVTA MDSVDARFWE AVEREDLEAL AGTLRSDDAD ELSSLGAVLP MLSSWRRRQG ERSTVDAWRY RVSWKPVTPT RGAGTLSGRW LVVAPADGAD DVWAEGAVRT LSARGAEVRR VELTAGDRTG MAERLREAVA DAPVAGVFAL PSLAGGADGL LRTAALAQAL GDLGVAAPLW VATRGAVSTG RSDGPVDPWQ ARVWGLGRVA ALELAERWGG LVDLPETADD RALSRLAGVL AGGNGAEGED QVAVRASGVF GRRLTRATAE TPGRDWRPGA GTVLVTGGTG ALGAHVARWL IGNGAEHVVL TSRRGLDAPG AAELRDELTA QGARITVAAC DAADRDALAA LVAGLPEELP LTAVVHAAGV LDDGVLESLT PERFATVLRA KADAAANLHE VTRELDLTAF VLFSSFAGSM GAAGQANYAA ANAFLDALAE RRRAEGLPAT SVAWGPWAEG GMAADDALEQ RMRRGGVPPM AADLAVAALQ RALDLDETTV MVADIDWERL APGFAASRPS PLLRELPEAR TALEAAAGQT ARTAGGGADE SALAQRLAGV PAAERERVLL DLVRSAVADV LGHADAEAVE ASRAFSELGF DSLTAVELRN RLNTVTGLRL PPTLVYDYPT SSALAAHLRT EILGEEGAAD TATTASSVGV AVAAVDDPIA IVGMSCRFPG RVDSPEELWR LLSTGQDAVT EFPADRGWDI DSLYDPEPGT EYTTYVREGG FLSDAGDFDA AFFGISPREA LAMDPQQRLL LETSWEAFER AGIDPTTLRG QQVGVFAGTN GQDYLSLVLQ SQDSGDGFMG TGNAASVLSG RVSYALGLEG PAVTVDTACS SSLVALHWAI QALRAGECSL ALAGGVTVMS TPGSFVDFSR QRGLAADGRI KAFAAGADGT GWGEGVGMLL VERLSDARRH GHTVLATVRG SAINQDGASN GLTAPNGPSQ QRVIRQALAG AGLSASEVDA VEAHGTGTTL GDPIEAQALL ATYGQDRSEG QPLLLGSIKS NIGHTQAAAG VAGVMKMVLA LQHGVLPQTL HVDEPTPHVD WSAGELELLT EAREWPETGR PRRAAVSSFG ISGTNAHTVL EQAPAPEEAP AAEPAAVLPT VPWVLSGKSR AAVRGQAARL LAHLGGDAAP ADVAYSLATT RTAMDHRAVL TADGAEGLLA GLAALARGES EVAGLVEGSV AEGGKVAFLF TGQGSQRLGM GRELYEAYPV FADALDEVCA HLDAHLERPL RDVLFGEDAA ALDRTGFTQP ALFAIEVALF RLVEAWGVKA DFLSGHSIGE LAAAHVAGVL SLADAATLVA ARGRLMQELP AGGAMIAVQA SEDEVVPLLT DRVSIAALNG PTSVVIAGDE DDAVQIAAFF ESQGRKTKRL TVSHAFHSPR MDGMLEAFRE VAQGLSYEAP RIPIVSHLTG DVVSADDITS PDFWVRHVRE AVRFLDGVRT LEARGVTTYV ELGPDGVLTA MAQECVTGDD EAAFVSALRK DRPEAEALTT ALARAHVRGV AVDWEAYFAG TGARKVALPT YAFQRSRYWV DALLTLDEVA SAGLGSAGHP LLGAAVELPD KDAFLFSGRL ALASHSWLAD HTVADTAVLP GSAFVELAVR AGDQVGCDRV DELALEEPLI LPERGGVQLR VSVGEPDGEG RRPLAVYSRP EGAESDQPWT RHAGGVLGTA EGRAAAEAGA FAVWPPADAE PVDTGALYAG MARAGLLHGP AFQSLRHVWR RGEELYAEVR IGEERQADAA RFALHPALLD GALQPLGLGV DGAAVAASWR GLSLYAVGAE ALRVRLTPAA DGTTRLVAAD ETGALVASVD ALAAEPLDAD RIAGASATSG RQDTLFRVDW APVAATSEAP SAGFAVVGGD DLKLSVALDA AGHTATVHDD LVALDAADGT APDTVLVPFL PETGTDRSDG LVADAHAATH RALGLVQEWL NGGRPGSARL VLVTRGAVAA AGDTEVTDLV NAPVWGLLRS AQTENPGQFV LLDVDGEVSY PAIAAALDSG EPEIALRAGA AYAPRLARVE APAAEPVRGA IDPEGTVLIT GATGGLGRLV ARHLATAHGA RHLLLASRSG PAAEGMAELT AELAELGTEA TVVACDAADR EALAGLLAGI PAERPLTAVV HTAGVLDDGV IDMLTPERVD RVLRPKVDAA VNLHELTREL DLSAFVLFSA AAGTIGGAGQ GGYAAANVFL DALAGHRRAT GRTAQSLVWG MWAEERGMGG RIGAADLKRA ARGGVLPLSA EEGLALFDAA RAVPDEPVLV PVRLDLAALR GAASAGELLP LYRGLVRTPV RRAADSGGPA VAETADGTGA DTLERQLARQ SEAQQERTVL DLVRRQVAAV LGYASKEQVG AEQAFKELGF DSLTAVELRN RLNAATGLKL TATLVYDYPT PMALARQLRH EMAPEAADSG MTLTDELERL ENSLSSLTPE DLAAVAGDEA AHANIAVRLQ TLLAKWHDVR RTADDGADAE SDAFEEVSDD ELFDFIDKRF GNG //