ID A0A1J0VBV3_9GAMM Unreviewed; 233 AA. AC A0A1J0VBV3; DT 15-FEB-2017, integrated into UniProtKB/TrEMBL. DT 15-FEB-2017, sequence version 1. DT 13-SEP-2023, entry version 27. DE RecName: Full=7-cyano-7-deazaguanine synthase {ECO:0000256|HAMAP-Rule:MF_01633}; DE EC=6.3.4.20 {ECO:0000256|HAMAP-Rule:MF_01633}; DE AltName: Full=7-cyano-7-carbaguanine synthase {ECO:0000256|HAMAP-Rule:MF_01633}; DE AltName: Full=PreQ(0) synthase {ECO:0000256|HAMAP-Rule:MF_01633}; DE AltName: Full=Queuosine biosynthesis protein QueC {ECO:0000256|HAMAP-Rule:MF_01633}; GN Name=queC {ECO:0000256|HAMAP-Rule:MF_01633}; GN ORFNames=BOX17_00080 {ECO:0000313|EMBL:APE29495.1}; OS Halomonas aestuarii. OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales; OC Halomonadaceae; Halomonas. OX NCBI_TaxID=1897729 {ECO:0000313|EMBL:APE29495.1, ECO:0000313|Proteomes:UP000181985}; RN [1] {ECO:0000313|Proteomes:UP000181985} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Hb3 {ECO:0000313|Proteomes:UP000181985}; RA Koh H.-W., Rani S., Park S.-J.; RT "Halolamina sediminis sp. nov., an extremely halophilic archaeon isolated RT from solar salt."; RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the ATP-dependent conversion of 7-carboxy-7- CC deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)). CC {ECO:0000256|HAMAP-Rule:MF_01633}. CC -!- CATALYTIC ACTIVITY: CC Reaction=7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7- CC deazaguanine + ADP + H(+) + H2O + phosphate; Xref=Rhea:RHEA:27982, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:45075, CC ChEBI:CHEBI:61036, ChEBI:CHEBI:456216; EC=6.3.4.20; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01633}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01633}; CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP-Rule:MF_01633}; CC -!- PATHWAY: Purine metabolism; 7-cyano-7-deazaguanine biosynthesis. CC {ECO:0000256|HAMAP-Rule:MF_01633}. CC -!- SIMILARITY: Belongs to the QueC family. {ECO:0000256|HAMAP- CC Rule:MF_01633}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP018139; APE29495.1; -; Genomic_DNA. DR RefSeq; WP_071941477.1; NZ_CP018139.1. DR AlphaFoldDB; A0A1J0VBV3; -. DR EnsemblBacteria; APE29495; APE29495; BOX17_00080. DR KEGG; hsi:BOX17_00080; -. DR OrthoDB; 9789567at2; -. DR UniPathway; UPA00391; -. DR Proteomes; UP000181985; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0008616; P:queuosine biosynthetic process; IEA:UniProtKB-UniRule. DR CDD; cd01995; ExsB; 1. DR Gene3D; 3.40.50.620; HUPs; 1. DR HAMAP; MF_01633; QueC; 1. DR InterPro; IPR018317; QueC. DR InterPro; IPR014729; Rossmann-like_a/b/a_fold. DR NCBIfam; TIGR00364; 7-cyano-7-deazaguanine synthase QueC; 1. DR PANTHER; PTHR42914; 7-CYANO-7-DEAZAGUANINE SYNTHASE; 1. DR PANTHER; PTHR42914:SF1; 7-CYANO-7-DEAZAGUANINE SYNTHASE; 1. DR Pfam; PF06508; QueC; 1. DR PIRSF; PIRSF006293; ExsB; 1. DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01633}; KW Ligase {ECO:0000256|HAMAP-Rule:MF_01633}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_01633}; KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01633}; KW Queuosine biosynthesis {ECO:0000256|ARBA:ARBA00022785, ECO:0000256|HAMAP- KW Rule:MF_01633}; Zinc {ECO:0000256|HAMAP-Rule:MF_01633}. FT BINDING 13..23 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01633" FT BINDING 190 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01633" FT BINDING 198 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01633" FT BINDING 201 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01633" FT BINDING 204 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /evidence="ECO:0000256|HAMAP-Rule:MF_01633" SQ SEQUENCE 233 AA; 25064 MW; 786D92F4893DB417 CRC64; MTHSPDPATV VIYSGGMDSF TVLHRALREG LDVHALSFDY GQRHRRELEV AERVCRELGV PHQVVDITAI HGLIDNSALT DASRAMPEGD YAAENLADTV VPNRNMILLS LAIAKAVNIG AGRVDYGAHG GDHVLYPDCR PEFVQAMNDV AGIANFEPLT IHAPYLRASK AEILAEGLAM GLDYADTWTC YRGEALACGR CGSCRERLAA FAANGATDPL GYSERPDLDE GAP //