ID A0A1H4CC04_9MYCO Unreviewed; 286 AA. AC A0A1H4CC04; DT 22-NOV-2017, integrated into UniProtKB/TrEMBL. DT 22-NOV-2017, sequence version 1. DT 16-JAN-2019, entry version 9. DE RecName: Full=Bifunctional protein FolD {ECO:0000256|HAMAP-Rule:MF_01576}; DE Includes: DE RecName: Full=Methenyltetrahydrofolate cyclohydrolase {ECO:0000256|HAMAP-Rule:MF_01576}; DE EC=3.5.4.9 {ECO:0000256|HAMAP-Rule:MF_01576}; DE Includes: DE RecName: Full=Methylenetetrahydrofolate dehydrogenase {ECO:0000256|HAMAP-Rule:MF_01576}; DE EC=1.5.1.5 {ECO:0000256|HAMAP-Rule:MF_01576}; GN Name=folD {ECO:0000256|HAMAP-Rule:MF_01576}; GN ORFNames=SAMN04488580_103340 {ECO:0000313|EMBL:SEA57829.1}; OS Mycobacterium sp. 283mftsu. OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; OC Mycobacterium. OX NCBI_TaxID=1761810 {ECO:0000313|EMBL:SEA57829.1, ECO:0000313|Proteomes:UP000199547}; RN [1] {ECO:0000313|EMBL:SEA57829.1, ECO:0000313|Proteomes:UP000199547} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=283MFTsu {ECO:0000313|EMBL:SEA57829.1, RC ECO:0000313|Proteomes:UP000199547}; RA de Groot N.N.; RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the oxidation of 5,10- CC methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and CC then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- CC formyltetrahydrofolate. {ECO:0000256|HAMAP-Rule:MF_01576}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = CC 5,10-methenyltetrahydrofolate + NADPH; Xref=Rhea:RHEA:22812, CC ChEBI:CHEBI:15636, ChEBI:CHEBI:57455, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349; EC=1.5.1.5; Evidence={ECO:0000256|HAMAP- CC Rule:MF_01576}; CC -!- CATALYTIC ACTIVITY: CC Reaction=5,10-methenyltetrahydrofolate + H2O = (6S)-10- CC formyltetrahydrofolate + H(+); Xref=Rhea:RHEA:23700, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57454, CC ChEBI:CHEBI:57455; EC=3.5.4.9; Evidence={ECO:0000256|HAMAP- CC Rule:MF_01576}; CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion. CC {ECO:0000256|HAMAP-Rule:MF_01576}. CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_01576}. CC -!- SIMILARITY: Belongs to the tetrahydrofolate CC dehydrogenase/cyclohydrolase family. {ECO:0000256|HAMAP- CC Rule:MF_01576}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation CC of feature annotation. {ECO:0000256|HAMAP-Rule:MF_01576}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FNRC01000003; SEA57829.1; -; Genomic_DNA. DR RefSeq; WP_029118730.1; NZ_FNRC01000003.1. DR UniPathway; UPA00193; -. DR Proteomes; UP000199547; Unassembled WGS sequence. DR GO; GO:0004477; F:methenyltetrahydrofolate cyclohydrolase activity; IEA:UniProtKB-UniRule. DR GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:UniProtKB-UniRule. DR GO; GO:0000105; P:histidine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0006164; P:purine nucleotide biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway. DR HAMAP; MF_01576; THF_DHG_CYH; 1. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR000672; THF_DH/CycHdrlase. DR InterPro; IPR020630; THF_DH/CycHdrlase_cat_dom. DR InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. DR PANTHER; PTHR10025; PTHR10025; 1. DR Pfam; PF00763; THF_DHG_CYH; 1. DR Pfam; PF02882; THF_DHG_CYH_C; 1. DR PRINTS; PR00085; THFDHDRGNASE. DR SUPFAM; SSF51735; SSF51735; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576}; KW Complete proteome {ECO:0000313|Proteomes:UP000199547}; KW Histidine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576}; KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_01576, KW ECO:0000313|EMBL:SEA57829.1}; KW Methionine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576}; KW Multifunctional enzyme {ECO:0000256|HAMAP-Rule:MF_01576}; KW NADP {ECO:0000256|HAMAP-Rule:MF_01576}; KW One-carbon metabolism {ECO:0000256|HAMAP-Rule:MF_01576}; KW Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_01576}; KW Purine biosynthesis {ECO:0000256|HAMAP-Rule:MF_01576}. FT DOMAIN 6 121 THF_DHG_CYH. {ECO:0000259|Pfam:PF00763}. FT DOMAIN 128 277 THF_DHG_CYH_C. {ECO:0000259|Pfam: FT PF02882}. FT NP_BIND 166 168 NADP. {ECO:0000256|HAMAP-Rule:MF_01576}. FT BINDING 191 191 NADP. {ECO:0000256|HAMAP-Rule:MF_01576}. SQ SEQUENCE 286 AA; 30188 MW; 41761BB2986E5DD1 CRC64; MSASVMDGTA VANQILNETR YRAANFERSH GRKPCLATVL LGDDPASHTY VRMKANRCRS IGLDSKRRHL PETASTAQAV ELVTELANDN GVDGILVQHP MPRQIDEREV FEAITPGKDV DGVTYASFAT MALGGNGFQS CTPGGMMRLL DAYDVDPAGH HAVVIGRSPI LGKPIGMLLL GRNATVTYCH SKTTNLPAII AEADIVVAAV GHPELIKGDW IKRGAVVLDA GYNPGNIGDV EYSAATQRAS LITPVPGGVG PTTIAVLLAQ TVDAANRTAL GARRSN //