ID A0A1E7UBH3_9GAMM Unreviewed; 205 AA. AC A0A1E7UBH3; DT 18-JAN-2017, integrated into UniProtKB/TrEMBL. DT 18-JAN-2017, sequence version 1. DT 15-MAR-2017, entry version 3. DE RecName: Full=Ribosomal RNA small subunit methyltransferase G {ECO:0000256|HAMAP-Rule:MF_00074}; DE EC=2.1.1.170 {ECO:0000256|HAMAP-Rule:MF_00074}; DE AltName: Full=16S rRNA 7-methylguanosine methyltransferase {ECO:0000256|HAMAP-Rule:MF_00074}; DE Short=16S rRNA m7G methyltransferase {ECO:0000256|HAMAP-Rule:MF_00074}; GN Name=rsmG {ECO:0000256|HAMAP-Rule:MF_00074}; GN ORFNames=AS144_02545 {ECO:0000313|EMBL:OEZ33427.1}; OS Francisella endosymbiont of Amblyomma maculatum. OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; OC Francisellaceae. OX NCBI_TaxID=255919 {ECO:0000313|EMBL:OEZ33427.1, ECO:0000313|Proteomes:UP000175883}; RN [1] {ECO:0000313|EMBL:OEZ33427.1, ECO:0000313|Proteomes:UP000175883} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=FLE-Am {ECO:0000313|EMBL:OEZ33427.1, RC ECO:0000313|Proteomes:UP000175883}; RX PubMed=27645766; DOI=10.1038/srep33670; RA Gerhart J.G., Moses A.S., Raghavan R.; RT "A Francisella-like endosymbiont in the Gulf Coast tick evolved from a RT mammalian pathogen."; RL Sci. Rep. 6:33670-33670(2016). CC -!- FUNCTION: Specifically methylates the N7 position of guanine in CC position 527 of 16S rRNA. {ECO:0000256|HAMAP-Rule:MF_00074}. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(527) in 16S CC rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S CC rRNA. {ECO:0000256|HAMAP-Rule:MF_00074}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00074, CC ECO:0000256|SAAS:SAAS00702625}. CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA CC methyltransferase RsmG family. {ECO:0000256|HAMAP-Rule:MF_00074, CC ECO:0000256|SAAS:SAAS00702615}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation CC of feature annotation. {ECO:0000256|HAMAP-Rule:MF_00074}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:OEZ33427.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LNCT01000002; OEZ33427.1; -; Genomic_DNA. DR RefSeq; WP_070084071.1; NZ_LNCT01000002.1. DR Proteomes; UP000175883; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.50.150; -; 1. DR HAMAP; MF_00074; 16SrRNA_methyltr_G; 1. DR InterPro; IPR003682; rRNA_ssu_MeTfrase_G. DR InterPro; IPR029063; SAM-dependent_MTases. DR PANTHER; PTHR31760; PTHR31760; 1. DR Pfam; PF02527; GidB; 1. DR PIRSF; PIRSF003078; GidB; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR TIGRFAMs; TIGR00138; rsmG_gidB; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000175883}; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS00702616}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS00702619, ECO:0000313|EMBL:OEZ33427.1}; KW Reference proteome {ECO:0000313|Proteomes:UP000175883}; KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS00702649}; KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS00702627}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS00702631, ECO:0000313|EMBL:OEZ33427.1}. FT REGION 127 128 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_00074}. FT BINDING 76 76 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|HAMAP-Rule: FT MF_00074}. FT BINDING 81 81 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|HAMAP-Rule: FT MF_00074}. FT BINDING 140 140 S-adenosyl-L-methionine. FT {ECO:0000256|HAMAP-Rule:MF_00074}. SQ SEQUENCE 205 AA; 23446 MW; B1E9C7B8E2E6D548 CRC64; MDTMKDKIKQ VLNQLNIQAT DIQITQWLDY LKLLEKWNKV YNMTAIKNIN DMLVKHLFDS LVVAKYIKGN STIDVGTGGG LPGVVLAILY PQHQFTLVDS VGKKIIFLKN VKKTLGLANI QPLNSRVENL QGSFNNIISR AFSSVDTFYE LCKHLLTYTN QMLAMKGRDL EERNLESLPL NIEKYSIKVP FLNVERNLIV MRKKS //