ID A0A1E7UBH3_9GAMM Unreviewed; 205 AA. AC A0A1E7UBH3; DT 18-JAN-2017, integrated into UniProtKB/TrEMBL. DT 18-JAN-2017, sequence version 1. DT 11-DEC-2019, entry version 11. DE RecName: Full=Ribosomal RNA small subunit methyltransferase G {ECO:0000256|HAMAP-Rule:MF_00074}; DE EC=2.1.1.170 {ECO:0000256|HAMAP-Rule:MF_00074}; DE AltName: Full=16S rRNA 7-methylguanosine methyltransferase {ECO:0000256|HAMAP-Rule:MF_00074}; DE Short=16S rRNA m7G methyltransferase {ECO:0000256|HAMAP-Rule:MF_00074}; GN Name=rsmG {ECO:0000256|HAMAP-Rule:MF_00074}; GN ORFNames=AS144_02545 {ECO:0000313|EMBL:OEZ33427.1}; OS Francisella endosymbiont of Amblyomma maculatum. OC Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; OC Francisellaceae. OX NCBI_TaxID=255919 {ECO:0000313|EMBL:OEZ33427.1, ECO:0000313|Proteomes:UP000175883}; RN [1] {ECO:0000313|EMBL:OEZ33427.1, ECO:0000313|Proteomes:UP000175883} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=FLE-Am {ECO:0000313|EMBL:OEZ33427.1, RC ECO:0000313|Proteomes:UP000175883}; RX PubMed=27645766; DOI=10.1038/srep33670; RA Gerhart J.G., Moses A.S., Raghavan R.; RT "A Francisella-like endosymbiont in the Gulf Coast tick evolved from a RT mammalian pathogen."; RL Sci. Rep. 6:33670-33670(2016). CC -!- FUNCTION: Specifically methylates the N7 position of guanine in CC position 527 of 16S rRNA. {ECO:0000256|HAMAP-Rule:MF_00074}. CC -!- CATALYTIC ACTIVITY: CC Reaction=guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)- CC methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42732, Rhea:RHEA-COMP:10209, Rhea:RHEA-COMP:10210, CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, CC ChEBI:CHEBI:74480; EC=2.1.1.170; Evidence={ECO:0000256|HAMAP- CC Rule:MF_00074}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00074, CC ECO:0000256|SAAS:SAAS01091540}. CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RNA CC methyltransferase RsmG family. {ECO:0000256|HAMAP-Rule:MF_00074, CC ECO:0000256|SAAS:SAAS01091529}. CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00074}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:OEZ33427.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LNCT01000002; OEZ33427.1; -; Genomic_DNA. DR EnsemblBacteria; OEZ33427; OEZ33427; AS144_02545. DR Proteomes; UP000175883; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0070043; F:rRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-UniRule. DR HAMAP; MF_00074; 16SrRNA_methyltr_G; 1. DR InterPro; IPR003682; rRNA_ssu_MeTfrase_G. DR InterPro; IPR029063; SAM-dependent_MTases. DR PANTHER; PTHR31760; PTHR31760; 1. DR Pfam; PF02527; GidB; 1. DR PIRSF; PIRSF003078; GidB; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR TIGRFAMs; TIGR00138; rsmG_gidB; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00074, ECO:0000256|SAAS:SAAS01091534}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS01091532, ECO:0000313|EMBL:OEZ33427.1}; KW rRNA processing {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS01091530}; KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS01091525}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_00074, KW ECO:0000256|SAAS:SAAS01091535, ECO:0000313|EMBL:OEZ33427.1}. FT REGION 127..128 FT /note="S-adenosyl-L-methionine binding" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00074" FT BINDING 76 FT /note="S-adenosyl-L-methionine; via carbonyl oxygen" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00074" FT BINDING 81 FT /note="S-adenosyl-L-methionine; via carbonyl oxygen" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00074" FT BINDING 140 FT /note="S-adenosyl-L-methionine" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00074" SQ SEQUENCE 205 AA; 23446 MW; B1E9C7B8E2E6D548 CRC64; MDTMKDKIKQ VLNQLNIQAT DIQITQWLDY LKLLEKWNKV YNMTAIKNIN DMLVKHLFDS LVVAKYIKGN STIDVGTGGG LPGVVLAILY PQHQFTLVDS VGKKIIFLKN VKKTLGLANI QPLNSRVENL QGSFNNIISR AFSSVDTFYE LCKHLLTYTN QMLAMKGRDL EERNLESLPL NIEKYSIKVP FLNVERNLIV MRKKS //