ID A0A1E7TUG3_9BURK Unreviewed; 329 AA. AC A0A1E7TUG3; DT 18-JAN-2017, integrated into UniProtKB/TrEMBL. DT 18-JAN-2017, sequence version 1. DT 12-AUG-2020, entry version 20. DE RecName: Full=DNA-directed RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00015972, ECO:0000256|HAMAP-Rule:MF_00059}; DE Short=RNAP subunit alpha {ECO:0000256|HAMAP-Rule:MF_00059}; DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418, ECO:0000256|HAMAP-Rule:MF_00059}; DE AltName: Full=RNA polymerase subunit alpha {ECO:0000256|ARBA:ARBA00016404, ECO:0000256|HAMAP-Rule:MF_00059}; DE AltName: Full=Transcriptase subunit alpha {ECO:0000256|ARBA:ARBA00021853, ECO:0000256|HAMAP-Rule:MF_00059}; GN Name=rpoA {ECO:0000256|HAMAP-Rule:MF_00059}; GN ORFNames=AO062_27730 {ECO:0000313|EMBL:OEZ27482.1}, CKY39_31435 GN {ECO:0000313|EMBL:ATA57236.1}, VCH24_57650 GN {ECO:0000313|EMBL:GER20723.1}, VHAB30_59940 GN {ECO:0000313|EMBL:GER14797.1}; OS Variovorax boronicumulans. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Comamonadaceae; Variovorax. OX NCBI_TaxID=436515 {ECO:0000313|EMBL:OEZ27482.1, ECO:0000313|Proteomes:UP000175920}; RN [1] {ECO:0000313|Proteomes:UP000175920} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=PHE5-4 {ECO:0000313|Proteomes:UP000175920}; RA Tabata M.; RT "Whole genome sequence of Cupriavidus necator strain PHE3-6."; RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:OEZ27482.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=PHE5-4 {ECO:0000313|EMBL:OEZ27482.1}; RA Jackson K.R., Lunt B.L., Fisher J.N.B., Gardner A.V., Bailey M.E., RA Deus L.M., Earl A.S., Gibby P.D., Hartmann K.A., Liu J.E., Manci A.M., RA Nielsen D.A., Solomon M.B., Breakwell D.P., Burnett S.H., Grose J.H.; RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|EMBL:ATA57236.1, ECO:0000313|Proteomes:UP000217154} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=J1 {ECO:0000313|EMBL:ATA57236.1, RC ECO:0000313|Proteomes:UP000217154}; RA Sun S.; RT "The diverse metabolic capabilities of V. boronicumulans make it an RT excellent choice for continued studies on novel biodegradation."; RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases. RN [4] {ECO:0000313|EMBL:GER20723.1, ECO:0000313|Proteomes:UP000430882} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=c24 {ECO:0000313|Proteomes:UP000430882}, and C24 RC {ECO:0000313|EMBL:GER20723.1}; RA Moriuchi R., Dohra H., Azwani F., Futamata H.; RT "Whole Genome Shotgun Sequence of Variovorax boronicumulans strain RT chemo24."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. RN [5] {ECO:0000313|EMBL:GER14797.1, ECO:0000313|Proteomes:UP000441010} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HAB-30 {ECO:0000313|EMBL:GER14797.1, RC ECO:0000313|Proteomes:UP000441010}; RA Moriuchi R., Dohra H., Azwani F., Futamata H.; RT "Whole Genome Shotgun Sequence of Variovorax boronicumulans strain HAB30."; RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of CC DNA into RNA using the four ribonucleoside triphosphates as substrates. CC {ECO:0000256|HAMAP-Rule:MF_00059}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- CC COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400; CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00000097, CC ECO:0000256|HAMAP-Rule:MF_00059}; CC -!- SUBUNIT: Homodimer. The RNAP catalytic core consists of 2 alpha, 1 CC beta, 1 beta' and 1 omega subunit. When a sigma factor is associated CC with the core the holoenzyme is formed, which can initiate CC transcription. {ECO:0000256|HAMAP-Rule:MF_00059}. CC -!- DOMAIN: The N-terminal domain is essential for RNAP assembly and basal CC transcription, whereas the C-terminal domain is involved in interaction CC with transcriptional regulators and with upstream promoter elements. CC {ECO:0000256|HAMAP-Rule:MF_00059}. CC -!- SIMILARITY: Belongs to the RNA polymerase alpha chain family. CC {ECO:0000256|ARBA:ARBA00007123, ECO:0000256|HAMAP-Rule:MF_00059}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP023284; ATA57236.1; -; Genomic_DNA. DR EMBL; BKDH01000006; GER14797.1; -; Genomic_DNA. DR EMBL; BKDI01000007; GER20723.1; -; Genomic_DNA. DR EMBL; LKDZ01000048; OEZ27482.1; -; Genomic_DNA. DR RefSeq; WP_056576973.1; NZ_MRCN01000023.1. DR EnsemblBacteria; OEZ27482; OEZ27482; AO062_27730. DR KEGG; vbo:CKY39_31435; -. DR KO; K03040; -. DR BioCyc; GCF_002291405:CKY39_RS31435-MONOMER; -. DR Proteomes; UP000175920; Unassembled WGS sequence. DR Proteomes; UP000217154; Chromosome. DR Proteomes; UP000430882; Unassembled WGS sequence. DR Proteomes; UP000441010; Unassembled WGS sequence. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule. DR Gene3D; 2.170.120.12; -; 1. DR Gene3D; 3.30.1360.10; -; 1. DR HAMAP; MF_00059; RNApol_bact_RpoA; 1. DR InterPro; IPR011262; DNA-dir_RNA_pol_insert. DR InterPro; IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3. DR InterPro; IPR011773; DNA-dir_RpoA. DR InterPro; IPR036603; RBP11-like. DR InterPro; IPR011260; RNAP_asu_C. DR InterPro; IPR036643; RNApol_insert_sf. DR Pfam; PF01000; RNA_pol_A_bac; 1. DR Pfam; PF03118; RNA_pol_A_CTD; 1. DR Pfam; PF01193; RNA_pol_L; 1. DR SMART; SM00662; RPOLD; 1. DR SUPFAM; SSF55257; SSF55257; 1. DR SUPFAM; SSF56553; SSF56553; 1. DR TIGRFAMs; TIGR02027; rpoA; 1. PE 3: Inferred from homology; KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478, KW ECO:0000256|HAMAP-Rule:MF_00059, ECO:0000313|EMBL:OEZ27482.1}; KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP- KW Rule:MF_00059, ECO:0000313|EMBL:OEZ27482.1}; KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP- KW Rule:MF_00059}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP- KW Rule:MF_00059, ECO:0000313|EMBL:OEZ27482.1}. FT DOMAIN 20..230 FT /note="RPOLD" FT /evidence="ECO:0000259|SMART:SM00662" FT REGION 1..231 FT /note="Alpha N-terminal domain (alpha-NTD)" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00059" FT REGION 249..329 FT /note="Alpha C-terminal domain (alpha-CTD)" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00059" SQ SEQUENCE 329 AA; 36168 MW; 160A4A3E1695E6C2 CRC64; MQTTLLKPKT IQVEQLAANK AKVTLEPFER GYGHTLGNAL RRVLLSSMVG YSATEVTIAG VLHEYSSIDG VQEDVVNILL NLKGVVFKLH NRDEVTLSLR KDGEGPVLAS DIQTPHDVEI INPDHVIAHL SQGGKLDMQI KVEKGRGYVP GTMRRYADEP TKSIGRIVLD ASFSPVKRVS YTVESARVEQ RTDLDKLLVE IETNGAISAE DAVRASAKIL VEQLAVFAQL EGGELAAFDA PAPRSAQQFD PILLRPVDEL ELTVRSANCL KAENIYYIGD LIQRTENELL KTPNLGRKSL NEIKEVLASR GLTLGMKLES WPPAGLDKR //