ID   A0A1D7PU60_ECOLX        Unreviewed;       345 AA.
AC   A0A1D7PU60;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   17-JUN-2020, entry version 30.
DE   RecName: Full=tRNA-dihydrouridine(20/20a) synthase {ECO:0000256|HAMAP-Rule:MF_02041};
DE            EC=1.3.1.91 {ECO:0000256|HAMAP-Rule:MF_02041};
DE   AltName: Full=U20-specific dihydrouridine synthase {ECO:0000256|HAMAP-Rule:MF_02041};
DE            Short=U20-specific Dus {ECO:0000256|HAMAP-Rule:MF_02041};
DE   AltName: Full=tRNA-dihydrouridine synthase A {ECO:0000256|HAMAP-Rule:MF_02041};
GN   Name=dusA {ECO:0000256|HAMAP-Rule:MF_02041,
GN   ECO:0000313|EMBL:KAB0088941.1};
GN   ORFNames=AW106_02855 {ECO:0000313|EMBL:OTE02434.1}, C9Z89_05935
GN   {ECO:0000313|EMBL:TJP30081.1}, COD30_15425
GN   {ECO:0000313|EMBL:PBR09483.1}, D5H35_01880
GN   {ECO:0000313|EMBL:EAB6609185.1}, D6004_01820
GN   {ECO:0000313|EMBL:EAB7434224.1}, D9610_00705
GN   {ECO:0000313|EMBL:EAB5834316.1}, DAH32_05130
GN   {ECO:0000313|EMBL:TGG14110.1}, DL530_02220
GN   {ECO:0000313|EMBL:EAA1302589.1}, DLW60_00750
GN   {ECO:0000313|EMBL:EAA0807158.1}, DM155_00750
GN   {ECO:0000313|EMBL:EAA1893921.1}, DQF71_02400
GN   {ECO:0000313|EMBL:EAA4933766.1}, DS143_02000
GN   {ECO:0000313|EMBL:EAC1021166.1}, DVB38_00750
GN   {ECO:0000313|EMBL:EAC0770733.1}, EAN70_04265
GN   {ECO:0000313|EMBL:EAB0590550.1}, EBM08_00695
GN   {ECO:0000313|EMBL:EAC0705808.1}, ED600_11985
GN   {ECO:0000313|EMBL:MFY70004.1}, EG599_11140
GN   {ECO:0000313|EMBL:EAB0452968.1}, EH412_04405
GN   {ECO:0000313|EMBL:EAB1107186.1}, EHJ36_05690
GN   {ECO:0000313|EMBL:EAB1133136.1}, EKI52_13580
GN   {ECO:0000313|EMBL:QBO39927.1}, F7F00_04860
GN   {ECO:0000313|EMBL:KAB0225011.1}, F7F23_02545
GN   {ECO:0000313|EMBL:KAB0088941.1};
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562 {ECO:0000313|EMBL:KAB0088941.1, ECO:0000313|Proteomes:UP000325452};
RN   [1] {ECO:0000313|EMBL:OTE02434.1, ECO:0000313|Proteomes:UP000194319}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OLC1257 {ECO:0000313|EMBL:OTE02434.1,
RC   ECO:0000313|Proteomes:UP000194319};
RA   Carrillo C.D., Koziol A.G., Mathews A., Goji N., Lambert D.,
RA   Huszczynski G., Deschenes M., Gauthier M., Amoako K., Blais B.;
RT   "Application of genomics in the determination of verotoxigenic Escherichia
RT   coli toxin subtypes.";
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:PBR09483.1, ECO:0000313|Proteomes:UP000218167}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=94-0426 {ECO:0000313|EMBL:PBR09483.1,
RC   ECO:0000313|Proteomes:UP000218167};
RA   Baranzoni G.M., Fratamico P.M., Reichenberger E.R., Kim G.-H., Funk J.,
RA   Manning S., Bosilevac J.M.;
RT   "Draft genomes of Shiga toxin-producing E. coli isolated from swine and
RT   other sources.";
RL   Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EMBL:TGG14110.1, ECO:0000313|Proteomes:UP000297292}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DEC10B {ECO:0000313|EMBL:TGG14110.1,
RC   ECO:0000313|Proteomes:UP000297292};
RA   Baranzoni G.M., Fratamico P.M., Kim G.-H., Funk J.A., Manning S.D.,
RA   Bosilevac J.M.;
RT   "Whole genome sequences of Shiga Toxin-producing Escherichia coli isolated
RT   from swine and other sources.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [4] {ECO:0000313|EMBL:EAB5834316.1, ECO:0000313|Proteomes:UP000370116, ECO:0000313|Proteomes:UP000373983}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=315225 {ECO:0000313|EMBL:EAA4933766.1,
RC   ECO:0000313|Proteomes:UP000392202}, 399915
RC   {ECO:0000313|EMBL:EAA0807158.1, ECO:0000313|Proteomes:UP000376932},
RC   410823 {ECO:0000313|EMBL:EAA1893921.1,
RC   ECO:0000313|Proteomes:UP000404067}, 488640
RC   {ECO:0000313|EMBL:EAA1302589.1, ECO:0000313|Proteomes:UP000341096},
RC   563007 {ECO:0000313|EMBL:EAC1021166.1,
RC   ECO:0000313|Proteomes:UP000367299}, 570834
RC   {ECO:0000313|EMBL:EAC0770733.1, ECO:0000313|Proteomes:UP000407349},
RC   591860 {ECO:0000313|EMBL:EAB6609185.1,
RC   ECO:0000313|Proteomes:UP000363044}, 608445
RC   {ECO:0000313|EMBL:EAB7434224.1, ECO:0000313|Proteomes:UP000370801},
RC   620380 {ECO:0000313|EMBL:EAB5834316.1,
RC   ECO:0000313|Proteomes:UP000370116}, and 624508
RC   {ECO:0000313|EMBL:EAC0705808.1, ECO:0000313|Proteomes:UP000373983};
RA   Ashton P.M., Dallman T., Nair S., De Pinna E., Peters T., Grant K.;
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN   [5] {ECO:0000313|Proteomes:UP000332547, ECO:0000313|Proteomes:UP000406531, ECO:0000313|Proteomes:UP000413862}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PNUSAE017386 {ECO:0000313|EMBL:EAB1133136.1,
RC   ECO:0000313|Proteomes:UP000413862}, PNUSAE018928
RC   {ECO:0000313|EMBL:EAB0590550.1, ECO:0000313|Proteomes:UP000330908},
RC   PNUSAE019399 {ECO:0000313|EMBL:MFY70004.1,
RC   ECO:0000313|Proteomes:UP000279100}, PNUSAE019781
RC   {ECO:0000313|EMBL:EAB0452968.1, ECO:0000313|Proteomes:UP000406531},
RC   and PNUSAE020430 {ECO:0000313|EMBL:EAB1107186.1,
RC   ECO:0000313|Proteomes:UP000332547};
RG   PulseNet: The National Subtyping Network for Foodborne Disease Surveillance;
RA   Tarr C.L., Trees E., Katz L.S., Carleton-Romer H.A., Stroika S.,
RA   Kucerova Z., Roache K.F., Sabol A.L., Besser J., Gerner-Smidt P.;
RL   Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
RN   [6] {ECO:0000313|EMBL:TJP30081.1, ECO:0000313|Proteomes:UP000306076}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FWSEC0139 {ECO:0000313|EMBL:TJP30081.1,
RC   ECO:0000313|Proteomes:UP000306076};
RA   Tyson S., Peterson C.-L., Olson A., Tyler S., Cabral J., Lynch T., Knox N.,
RA   Van Domselaar G., Graham M.;
RT   "Food and Water Safety Consortium.";
RL   Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
RN   [7] {ECO:0000313|EMBL:QBO39927.1, ECO:0000313|Proteomes:UP000292460}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFSAN027346 {ECO:0000313|EMBL:QBO39927.1,
RC   ECO:0000313|Proteomes:UP000292460};
RA   Gonzalez-Escalona N., Hoffmann M.;
RT   "Nanopore sequencing for fast determination of plasmids, phages, virulence
RT   markers, and antimicrobial resistance genes in Shiga toxin-producing
RT   Escherichia coli.";
RL   Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
RN   [8] {ECO:0000313|EMBL:KAB0088941.1, ECO:0000313|Proteomes:UP000325452, ECO:0000313|Proteomes:UP000338460}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=STEC_180185 {ECO:0000313|EMBL:KAB0088941.1,
RC   ECO:0000313|Proteomes:UP000325452}, and STEC_180784
RC   {ECO:0000313|EMBL:KAB0225011.1, ECO:0000313|Proteomes:UP000338460};
RA   Abdalhamid B., Mccutchen E.L., Bouska A.C., Hinrichs S.H., Iwen P.C.;
RT   "Whole Genome Sequencing of Shiga-Toxin Escherichia coli Strains from
RT   Nebraska.";
RL   Submitted (MAR-2019) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified
CC       base found in the D-loop of most tRNAs, via the reduction of the C5-C6
CC       double bond in target uridines. Specifically modifies U20 and U20a in
CC       tRNAs. {ECO:0000256|HAMAP-Rule:MF_02041}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20) in tRNA + NAD(+) = H(+) + NADH +
CC         uridine(20) in tRNA; Xref=Rhea:RHEA:53340, Rhea:RHEA-COMP:13533,
CC         Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20) in tRNA + NADP(+) = H(+) + NADPH +
CC         uridine(20) in tRNA; Xref=Rhea:RHEA:53336, Rhea:RHEA-COMP:13533,
CC         Rhea:RHEA-COMP:13534, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443; EC=1.3.1.91;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20a) in tRNA + NAD(+) = H(+) + NADH +
CC         uridine(20a) in tRNA; Xref=Rhea:RHEA:53348, Rhea:RHEA-COMP:13535,
CC         Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,6-dihydrouridine(20a) in tRNA + NADP(+) = H(+) + NADPH +
CC         uridine(20a) in tRNA; Xref=Rhea:RHEA:53344, Rhea:RHEA-COMP:13535,
CC         Rhea:RHEA-COMP:13536, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:65315, ChEBI:CHEBI:74443;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_02041};
CC   -!- COFACTOR:
CC       Name=FMN; Xref=ChEBI:CHEBI:58210;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_02041,
CC         ECO:0000256|PIRNR:PIRNR006621};
CC   -!- SIMILARITY: Belongs to the Dus family. DusA subfamily.
CC       {ECO:0000256|HAMAP-Rule:MF_02041}.
CC   -!- SIMILARITY: Belongs to the dus family. {ECO:0000256|PIRNR:PIRNR006621}.
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DR   EMBL; AAAAJM010000001; EAA0807158.1; -; Genomic_DNA.
DR   EMBL; AAAANM010000003; EAA1302589.1; -; Genomic_DNA.
DR   EMBL; AAAASG010000001; EAA1893921.1; -; Genomic_DNA.
DR   EMBL; AAABQM010000003; EAA4933766.1; -; Genomic_DNA.
DR   EMBL; AAADKN010000018; EAB0452968.1; -; Genomic_DNA.
DR   EMBL; AAADKK010000004; EAB0590550.1; -; Genomic_DNA.
DR   EMBL; AAADOF010000004; EAB1107186.1; -; Genomic_DNA.
DR   EMBL; AAADOH010000006; EAB1133136.1; -; Genomic_DNA.
DR   EMBL; AAAFEB010000001; EAB5834316.1; -; Genomic_DNA.
DR   EMBL; AAAFJW010000002; EAB6609185.1; -; Genomic_DNA.
DR   EMBL; AAAFQE010000002; EAB7434224.1; -; Genomic_DNA.
DR   EMBL; AAAGRT010000001; EAC0705808.1; -; Genomic_DNA.
DR   EMBL; AAAGSA010000001; EAC0770733.1; -; Genomic_DNA.
DR   EMBL; AAAGUG010000002; EAC1021166.1; -; Genomic_DNA.
DR   EMBL; VZEX01000003; KAB0088941.1; -; Genomic_DNA.
DR   EMBL; VZEA01000005; KAB0225011.1; -; Genomic_DNA.
DR   EMBL; RNIM01000025; MFY70004.1; -; Genomic_DNA.
DR   EMBL; NENZ01000004; OTE02434.1; -; Genomic_DNA.
DR   EMBL; NWBE01000019; PBR09483.1; -; Genomic_DNA.
DR   EMBL; CP037945; QBO39927.1; -; Genomic_DNA.
DR   EMBL; QAFD01000005; TGG14110.1; -; Genomic_DNA.
DR   EMBL; RRHE01000007; TJP30081.1; -; Genomic_DNA.
DR   RefSeq; WP_001432107.1; NZ_VODJ01000361.1.
DR   EnsemblBacteria; AOM48665; AOM48665; FORC28_5690.
DR   BioCyc; GCF_001721125:FORC28_RS27565-MONOMER; -.
DR   Proteomes; UP000194319; Unassembled WGS sequence.
DR   Proteomes; UP000218167; Unassembled WGS sequence.
DR   Proteomes; UP000279100; Unassembled WGS sequence.
DR   Proteomes; UP000292460; Chromosome.
DR   Proteomes; UP000297292; Unassembled WGS sequence.
DR   Proteomes; UP000306076; Unassembled WGS sequence.
DR   Proteomes; UP000325452; Unassembled WGS sequence.
DR   Proteomes; UP000330908; Unassembled WGS sequence.
DR   Proteomes; UP000332547; Unassembled WGS sequence.
DR   Proteomes; UP000338460; Unassembled WGS sequence.
DR   Proteomes; UP000341096; Unassembled WGS sequence.
DR   Proteomes; UP000363044; Unassembled WGS sequence.
DR   Proteomes; UP000367299; Unassembled WGS sequence.
DR   Proteomes; UP000370116; Unassembled WGS sequence.
DR   Proteomes; UP000370801; Unassembled WGS sequence.
DR   Proteomes; UP000373983; Unassembled WGS sequence.
DR   Proteomes; UP000376932; Unassembled WGS sequence.
DR   Proteomes; UP000392202; Unassembled WGS sequence.
DR   Proteomes; UP000404067; Unassembled WGS sequence.
DR   Proteomes; UP000406531; Unassembled WGS sequence.
DR   Proteomes; UP000407349; Unassembled WGS sequence.
DR   Proteomes; UP000413862; Unassembled WGS sequence.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0010181; F:FMN binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0102264; F:tRNA-dihydrouridine20 synthase activity; IEA:UniProtKB-EC.
DR   CDD; cd02801; DUS_like_FMN; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_02041; DusA_subfam; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR035587; DUS-like_FMN-bd.
DR   InterPro; IPR004653; DusA.
DR   InterPro; IPR001269; tRNA_hU_synthase.
DR   InterPro; IPR018517; tRNA_hU_synthase_CS.
DR   PANTHER; PTHR42907; PTHR42907; 1.
DR   Pfam; PF01207; Dus; 1.
DR   PIRSF; PIRSF006621; Dus; 1.
DR   TIGRFAMs; TIGR00742; yjbN; 1.
DR   PROSITE; PS01136; UPF0034; 1.
PE   3: Inferred from homology;
KW   Flavoprotein {ECO:0000256|HAMAP-Rule:MF_02041,
KW   ECO:0000256|PIRNR:PIRNR006621};
KW   FMN {ECO:0000256|HAMAP-Rule:MF_02041, ECO:0000256|PIRNR:PIRNR006621};
KW   NADP {ECO:0000256|HAMAP-Rule:MF_02041};
KW   Oxidoreductase {ECO:0000256|HAMAP-Rule:MF_02041,
KW   ECO:0000256|PIRNR:PIRNR006621, ECO:0000313|EMBL:KAB0088941.1};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_02041};
KW   tRNA processing {ECO:0000256|HAMAP-Rule:MF_02041,
KW   ECO:0000256|PIRNR:PIRNR006621};
KW   tRNA-binding {ECO:0000256|HAMAP-Rule:MF_02041}.
FT   NP_BIND         32..34
FT                   /note="FMN"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   NP_BIND         226..228
FT                   /note="FMN"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   NP_BIND         248..249
FT                   /note="FMN"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   ACT_SITE        114
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041,
FT                   ECO:0000256|PIRSR:PIRSR006621-1"
FT   BINDING         84
FT                   /note="FMN"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   BINDING         153
FT                   /note="FMN"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   BINDING         186
FT                   /note="FMN"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   SITE            111
FT                   /note="Interacts with tRNA"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   SITE            198
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   SITE            201
FT                   /note="Interacts with tRNA"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   SITE            314
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
FT   SITE            317
FT                   /note="Interacts with tRNA; defines subfamily-specific
FT                   binding signature"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_02041"
SQ   SEQUENCE   345 AA;  38498 MW;  80F7DF7EF1E96A28 CRC64;
     MHGNSEMQKI NQTSAMPEKT DVHWSGRFSV APMLDWTDRH CRYFLRLLSR NTLLYTEMVT
     TGAIIHGKGD YLAYSEEEHP VALQLGGSDP AALAQCAKLA EARGYDEINL NVGCPSDRVQ
     NGMFGACLMG NTQLVADCVK AMRDVVSIPV TVKTRIGIDD QDSYEFLCDF INTVSGKGEC
     EMFIIHARKA WLSGLSPKEN REIPPLDYPR VYQLKRDFPH LTMSINGGIK SLEEAKAHLQ
     HMDGVMVGRE AYQNPGILAA VDREIFGSSD TDADPVAVVR AMYPYIEREL SQGTYLGHIT
     RHMLGLFQGI PGARQWRRYL SENAHKAGAD INVLEHALKL VADKR
//