ID A0A1C7CCT6_PSEAI Unreviewed; 72 AA. AC A0A1C7CCT6; DT 02-NOV-2016, integrated into UniProtKB/TrEMBL. DT 02-NOV-2016, sequence version 1. DT 12-OCT-2022, entry version 22. DE RecName: Full=Translation initiation factor IF-1 {ECO:0000256|HAMAP-Rule:MF_00075}; GN Name=infA {ECO:0000256|HAMAP-Rule:MF_00075}; GN ORFNames=Q058_02013 {ECO:0000313|EMBL:ERV82923.1}; OS Pseudomonas aeruginosa BL04. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=1402545 {ECO:0000313|EMBL:ERV82923.1, ECO:0000313|Proteomes:UP000017518}; RN [1] {ECO:0000313|EMBL:ERV82923.1, ECO:0000313|Proteomes:UP000017518} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=BL04 {ECO:0000313|EMBL:ERV82923.1, RC ECO:0000313|Proteomes:UP000017518}; RG The Broad Institute Genomics Platform; RG The Broad Institute Genome Sequencing Center for Infectious Disease; RA Hung D., Penaranda C., Poulsen B., Young S.K., Zeng Q., Gargeya S., RA Fitzgerald M., Abouelleil A., Alvarado L., Chapman S.B., Gainer-Dewar J., RA Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A., RA Ireland A., Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W., RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C., RA Birren B.; RT "The Genome Sequence of Pseudomonas aeruginosa BL04."; RL Submitted (OCT-2013) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: One of the essential components for the initiation of protein CC synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit CC to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps CC modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). CC Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are CC released leaving the mature 70S translation initiation complex. CC {ECO:0000256|HAMAP-Rule:MF_00075}. CC -!- SUBUNIT: Component of the 30S ribosomal translation pre-initiation CC complex which assembles on the 30S ribosome in the order IF-2 and IF-3, CC IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at CC any time during PIC assembly. {ECO:0000256|HAMAP-Rule:MF_00075}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00075}. CC -!- SIMILARITY: Belongs to the IF-1 family. {ECO:0000256|HAMAP- CC Rule:MF_00075}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:ERV82923.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AXPW01000001; ERV82923.1; -; Genomic_DNA. DR RefSeq; WP_002553999.1; NZ_KI518931.1. DR SMR; A0A1C7CCT6; -. DR EnsemblBacteria; ERV82923; ERV82923; Q058_02013. DR GeneID; 61931168; -. DR GeneID; 64443124; -. DR GeneID; 72494622; -. DR PATRIC; fig|1402545.3.peg.2025; -. DR HOGENOM; CLU_151267_1_0_6; -. DR Proteomes; UP000017518; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0043022; F:ribosome binding; IEA:UniProtKB-UniRule. DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-UniRule. DR CDD; cd04451; S1_IF1; 1. DR Gene3D; 2.40.50.140; -; 1. DR HAMAP; MF_00075; IF_1; 1. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR006196; RNA-binding_domain_S1_IF1. DR InterPro; IPR022967; S1_dom. DR InterPro; IPR004368; TIF_IF1. DR PANTHER; PTHR33370; PTHR33370; 1. DR Pfam; PF01176; eIF-1a; 1. DR SMART; SM00316; S1; 1. DR SUPFAM; SSF50249; SSF50249; 1. DR TIGRFAMs; TIGR00008; infA; 1. DR PROSITE; PS50832; S1_IF1_TYPE; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00075}; KW Initiation factor {ECO:0000256|ARBA:ARBA00022540, ECO:0000256|HAMAP- KW Rule:MF_00075}; KW Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00075, ECO:0000256|PROSITE- KW ProRule:PRU00181}; RNA-binding {ECO:0000256|HAMAP-Rule:MF_00075}; KW rRNA-binding {ECO:0000256|HAMAP-Rule:MF_00075}. FT DOMAIN 1..72 FT /note="S1-like" FT /evidence="ECO:0000259|PROSITE:PS50832" SQ SEQUENCE 72 AA; 8303 MW; 97E5EEFD8F3886DB CRC64; MSKEDSFEME GTVVDTLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG DKVRVELTPY DLSKGRITYR AR //