ID A0A1B4Z9A6_9ACTN Unreviewed; 2480 AA. AC A0A1B4Z9A6; DT 02-NOV-2016, integrated into UniProtKB/TrEMBL. DT 02-NOV-2016, sequence version 1. DT 29-MAY-2024, entry version 34. DE SubName: Full=PKS (KS-AT-DH-ER-KR-ACP-TE) {ECO:0000313|EMBL:BAV56012.1}; GN Name=flvP3 {ECO:0000313|EMBL:BAV56012.1}; OS Actinomadura fulva subsp. indica. OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales; OC Thermomonosporaceae; Actinomadura. OX NCBI_TaxID=1752060 {ECO:0000313|EMBL:BAV56012.1}; RN [1] {ECO:0000313|EMBL:BAV56012.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ATCC 53714 {ECO:0000313|EMBL:BAV56012.1}; RX PubMed=26818633; DOI=10.1080/09168451.2015.1132155; RA Miyanaga A., Hayakawa Y., Numakura M., Hashimoto J., Teruya K., Hirano T., RA Shin-ya K., Kudo F., Eguchi T.; RT "Identification of the Fluvirucin B2 (Sch 38518) Biosynthetic Gene Cluster RT from Actinomadura fulva subsp. indica ATCC 53714: substrate Specificity of RT the ?-Amino Acid Selective Adenylating Enzyme FlvN."; RL Biosci. Biotechnol. Biochem. 80:935-941(2016). CC -!- FUNCTION: Fatty acid synthetase is a multifunctional enzyme that CC catalyzes the de novo biosynthesis of long-chain saturated fatty acids CC starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This CC multifunctional protein contains 7 catalytic activities and a site for CC the binding of the prosthetic group 4'-phosphopantetheine of the acyl CC carrier protein ([ACP]) domain. {ECO:0000256|ARBA:ARBA00023442}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-butenoyl-[ACP] + H(+) + NADPH = butanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41812, Rhea:RHEA-COMP:9627, Rhea:RHEA-COMP:9628, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78453, ChEBI:CHEBI:78454; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41813; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-decenoyl-[ACP] + H(+) + NADPH = decanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41864, Rhea:RHEA-COMP:9639, Rhea:RHEA-COMP:9640, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78467, ChEBI:CHEBI:78468; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41865; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-dodecenoyl-[ACP] + H(+) + NADPH = dodecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41880, Rhea:RHEA-COMP:9643, Rhea:RHEA- CC COMP:9644, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41881; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexadecenoyl-[ACP] + H(+) + NADPH = hexadecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41912, Rhea:RHEA-COMP:9651, Rhea:RHEA- CC COMP:9652, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78481, ChEBI:CHEBI:78483; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41913; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexenoyl-[ACP] + H(+) + NADPH = hexanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41832, Rhea:RHEA-COMP:9631, Rhea:RHEA-COMP:9632, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78458, ChEBI:CHEBI:78459; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41833; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octadecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC octadecanoyl-[ACP]; Xref=Rhea:RHEA:41928, Rhea:RHEA-COMP:9655, CC Rhea:RHEA-COMP:9656, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78489, ChEBI:CHEBI:78495; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41929; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octenoyl-[ACP] + H(+) + NADPH = NADP(+) + octanoyl-[ACP]; CC Xref=Rhea:RHEA:41848, Rhea:RHEA-COMP:9635, Rhea:RHEA-COMP:9636, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78462, ChEBI:CHEBI:78463; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41849; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-tetradecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC tetradecanoyl-[ACP]; Xref=Rhea:RHEA:41896, Rhea:RHEA-COMP:9647, CC Rhea:RHEA-COMP:9648, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78475, ChEBI:CHEBI:78477; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41897; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41808, Rhea:RHEA-COMP:9626, Rhea:RHEA-COMP:9627, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78451, ChEBI:CHEBI:78453; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41809; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41860, Rhea:RHEA-COMP:9638, Rhea:RHEA-COMP:9639, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78466, ChEBI:CHEBI:78467; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41861; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41876, Rhea:RHEA-COMP:9642, Rhea:RHEA-COMP:9643, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78470, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41877; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41908, Rhea:RHEA-COMP:9650, Rhea:RHEA-COMP:9651, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78480, ChEBI:CHEBI:78481; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41909; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41828, Rhea:RHEA-COMP:9630, Rhea:RHEA-COMP:9631, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78457, ChEBI:CHEBI:78458; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41829; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41924, Rhea:RHEA-COMP:9654, Rhea:RHEA-COMP:9655, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78488, ChEBI:CHEBI:78489; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41925; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41844, Rhea:RHEA-COMP:9634, Rhea:RHEA-COMP:9635, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78461, ChEBI:CHEBI:78462; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41845; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41892, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9647, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78474, ChEBI:CHEBI:78475; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41893; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxobutanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxybutanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41804, Rhea:RHEA-COMP:9625, CC Rhea:RHEA-COMP:9626, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78450, ChEBI:CHEBI:78451; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41805; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxodecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41856, Rhea:RHEA-COMP:9637, CC Rhea:RHEA-COMP:9638, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78464, ChEBI:CHEBI:78466; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41857; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxododecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydodecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41872, Rhea:RHEA-COMP:9641, CC Rhea:RHEA-COMP:9642, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78469, ChEBI:CHEBI:78470; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41873; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyhexadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41904, Rhea:RHEA- CC COMP:9649, Rhea:RHEA-COMP:9650, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78478, ChEBI:CHEBI:78480; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41905; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyhexanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41824, Rhea:RHEA-COMP:9629, CC Rhea:RHEA-COMP:9630, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78456, ChEBI:CHEBI:78457; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41825; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyoctadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41920, Rhea:RHEA- CC COMP:9653, Rhea:RHEA-COMP:9654, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78487, ChEBI:CHEBI:78488; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41921; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyoctanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41840, Rhea:RHEA-COMP:9633, CC Rhea:RHEA-COMP:9634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78460, ChEBI:CHEBI:78461; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41841; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxotetradecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxytetradecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41888, CC Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9646, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78473, CC ChEBI:CHEBI:78474; Evidence={ECO:0000256|ARBA:ARBA00023367}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41889; CC Evidence={ECO:0000256|ARBA:ARBA00023367}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41916, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9653, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78483, ChEBI:CHEBI:78487; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41917; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9632, Rhea:RHEA-COMP:9633, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78459, ChEBI:CHEBI:78460; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41837; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41852, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9636, Rhea:RHEA-COMP:9637, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78463, ChEBI:CHEBI:78464; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41853; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + tetradecanoyl-[ACP] = 3- CC oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41900, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9648, Rhea:RHEA-COMP:9649, CC Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78477, CC ChEBI:CHEBI:78478; Evidence={ECO:0000256|ARBA:ARBA00023341}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41901; CC Evidence={ECO:0000256|ARBA:ARBA00023341}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + hexadecanoyl-[ACP] = H(+) + hexadecanoate + holo-[ACP]; CC Xref=Rhea:RHEA:41932, Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78483; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41933; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + tetradecanoyl-[ACP] = H(+) + holo-[ACP] + CC tetradecanoate; Xref=Rhea:RHEA:30123, Rhea:RHEA-COMP:9648, Rhea:RHEA- CC COMP:9685, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78477; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30124; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) CC + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA- CC COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17399; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13098; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADPH; Xref=Rhea:RHEA:22564, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.39; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22566; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:22836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9916, Rhea:RHEA-COMP:14125, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78776, ChEBI:CHEBI:138651; CC EC=2.3.1.41; Evidence={ECO:0000256|ARBA:ARBA00023389}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22837; CC Evidence={ECO:0000256|ARBA:ARBA00023389}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + 2n H(+) + n malonyl-CoA + 2n NADPH = a long-chain CC fatty acid + n CO2 + (n+1) CoA + 2n NADP(+); Xref=Rhea:RHEA:14993, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57384, ChEBI:CHEBI:57560, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=2.3.1.85; CC Evidence={ECO:0000256|ARBA:ARBA00023370}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14994; CC Evidence={ECO:0000256|ARBA:ARBA00023370}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + holo-[ACP] = acetyl-[ACP] + CoA; CC Xref=Rhea:RHEA:41788, Rhea:RHEA-COMP:9621, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446; EC=2.3.1.38; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41789; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41800, Rhea:RHEA-COMP:9621, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9625, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446, ChEBI:CHEBI:78449, ChEBI:CHEBI:78450; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41801; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC -!- CATALYTIC ACTIVITY: CC Reaction=butanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41820, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9628, Rhea:RHEA-COMP:9629, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78454, ChEBI:CHEBI:78456; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41821; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC -!- CATALYTIC ACTIVITY: CC Reaction=decanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxododecanoyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41868, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9640, Rhea:RHEA-COMP:9641, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78468, ChEBI:CHEBI:78469; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41869; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC -!- CATALYTIC ACTIVITY: CC Reaction=dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41884, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9644, Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78449, ChEBI:CHEBI:78473; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41885; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LC095592; BAV56012.1; -; Genomic_DNA. DR GO; GO:0005737; C:cytoplasm; IEA:TreeGrafter. DR GO; GO:0005886; C:plasma membrane; IEA:TreeGrafter. DR GO; GO:0019171; F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0004316; F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; IEA:UniProtKB-EC. DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:UniProtKB-EC. DR GO; GO:0004313; F:[acyl-carrier-protein] S-acetyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0047117; F:enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; IEA:UniProtKB-EC. DR GO; GO:0004312; F:fatty acid synthase activity; IEA:UniProtKB-EC. DR GO; GO:0016297; F:fatty acyl-[ACP] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0071770; P:DIM/DIP cell wall layer assembly; IEA:TreeGrafter. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt. DR GO; GO:0009403; P:toxin biosynthetic process; IEA:UniProt. DR CDD; cd05195; enoyl_red; 1. DR CDD; cd08956; KR_3_FAS_SDR_x; 1. DR CDD; cd00833; PKS; 1. DR Gene3D; 3.30.70.3290; -; 1. DR Gene3D; 3.40.47.10; -; 1. DR Gene3D; 3.40.50.11460; -; 1. DR Gene3D; 1.10.1200.10; ACP-like; 1. DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1. DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1. DR Gene3D; 3.90.180.10; Medium-chain alcohol dehydrogenases, catalytic domain; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR001227; Ac_transferase_dom_sf. DR InterPro; IPR036736; ACP-like_sf. DR InterPro; IPR014043; Acyl_transferase. DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase. DR InterPro; IPR013154; ADH-like_N. DR InterPro; IPR011032; GroES-like_sf. DR InterPro; IPR018201; Ketoacyl_synth_AS. DR InterPro; IPR014031; Ketoacyl_synth_C. DR InterPro; IPR014030; Ketoacyl_synth_N. DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR032821; PKS_assoc. DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom. DR InterPro; IPR042104; PKS_dehydratase_sf. DR InterPro; IPR020807; PKS_DH. DR InterPro; IPR049551; PKS_DH_C. DR InterPro; IPR049552; PKS_DH_N. DR InterPro; IPR020843; PKS_ER. DR InterPro; IPR013968; PKS_KR. DR InterPro; IPR049900; PKS_mFAS_DH. DR InterPro; IPR020806; PKS_PP-bd. DR InterPro; IPR020802; PKS_thioesterase. DR InterPro; IPR015083; Polyketide_synth_docking. DR InterPro; IPR009081; PP-bd_ACP. DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS. DR InterPro; IPR001031; Thioesterase. DR InterPro; IPR016039; Thiolase-like. DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1. DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1. DR Pfam; PF00698; Acyl_transf_1; 1. DR Pfam; PF08240; ADH_N; 1. DR Pfam; PF13602; ADH_zinc_N_2; 1. DR Pfam; PF08990; Docking; 1. DR Pfam; PF16197; KAsynt_C_assoc; 1. DR Pfam; PF00109; ketoacyl-synt; 1. DR Pfam; PF02801; Ketoacyl-synt_C; 1. DR Pfam; PF08659; KR; 1. DR Pfam; PF21089; PKS_DH_N; 1. DR Pfam; PF00550; PP-binding; 1. DR Pfam; PF14765; PS-DH; 1. DR Pfam; PF00975; Thioesterase; 1. DR SMART; SM00827; PKS_AT; 1. DR SMART; SM00826; PKS_DH; 1. DR SMART; SM00829; PKS_ER; 1. DR SMART; SM00822; PKS_KR; 1. DR SMART; SM00825; PKS_KS; 1. DR SMART; SM00823; PKS_PP; 1. DR SMART; SM01294; PKS_PP_betabranch; 1. DR SMART; SM00824; PKS_TE; 1. DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1. DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1. DR SUPFAM; SSF50129; GroES-like; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 3. DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 1. DR SUPFAM; SSF53901; Thiolase-like; 1. DR PROSITE; PS50075; CARRIER; 1. DR PROSITE; PS00606; KS3_1; 1. DR PROSITE; PS52004; KS3_2; 1. DR PROSITE; PS52019; PKS_MFAS_DH; 1. DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1. PE 4: Predicted; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268}; KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898}; KW S-nitrosylation {ECO:0000256|ARBA:ARBA00022799}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 33..460 FT /note="Ketosynthase family 3 (KS3)" FT /evidence="ECO:0000259|PROSITE:PS52004" FT DOMAIN 1018..1292 FT /note="PKS/mFAS DH" FT /evidence="ECO:0000259|PROSITE:PS52019" FT DOMAIN 2103..2178 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT REGION 463..530 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 1018..1139 FT /note="N-terminal hotdog fold" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" FT REGION 1151..1292 FT /note="C-terminal hotdog fold" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" FT COMPBIAS 490..530 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT ACT_SITE 1050 FT /note="Proton acceptor; for dehydratase activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" FT ACT_SITE 1212 FT /note="Proton donor; for dehydratase activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01363" SQ SEQUENCE 2480 AA; 261632 MW; FE91EDE488158C28 CRC64; MADEEKLLAY LRRVTEDLQQ ANRRLRDVET KEREPIAIVS MACRYPGGVN SPEDLWNLVL NREQGIVPFP KDRGWDLEAL YDPDPDTSGT TYARAGGFLD GADEFDAAFF GISPREAVAM DPQQRVVLEV AWELMERAGL HPGSLRGTPT GVFIGAFAND YEVGRGPLPE GSEGYLGTGN SSSVISGRVA YTLGLEGPAV TVDTACSSSL VAIHLACQSL RNGESTLALA GGVTIMATPE LLVDFSHQRG LARDDRCKAF SDEADGTVLS EGVGVLLLER LSDALAGGRR VLGVIRGSAV NQDGASNGLT APNGPSQERV IRQALANAGV CAGEVDVVEA HGTGTALGDP IEAQALLATY GRDRDAGVPL WLGSVKSNIG HSQAAAGVAG VIKMVQAMRH GVLPQTLHVS APTSHVDWEG GGVRLLDRAR EWPRLGGRPR RAGVSSFGVS GTNAHVIVEQ APDPEPRQEQ APDAQPQQEQ APDGQPRQAF ELEPRQDVRP QEDTHEDLRE GAQEDVRAGA REDVRAGARE DVRAGAQEDL REGVRVGVPV VSGVVPWVVS GRGVAALRAQ AGRLVDFVEA RAGLPVAGVG LSLVRGRAVF EDRAVVLGGD RQELLTGLRS VAAGEPDTGP VPGPVSGVVS GVVSGSRDAV LVFPGQGAQW VGMGVELAQA SPVFAARLGE CFGEIGRWVE WDPAGVLADP AGAGLASIEV LQPVVFAVGV ALAALWESVG VRPAAVVGHS QGEVAAACVA GVLSLADAVR VVVLRSRLFA RRLQGRGAIA AVALPADVVR EEVAAWGGVL EVSADNGPTS CAVAGSLPAL EEFVERVRGR GVRARVIEAT VASHSSMVEP LRDELVGMLG GVAARQGRVP FYSTVTGGLV DGGGLGAEYW FANARRPVVF QQAVRALLAD GRTGFIEASP HPVLTMAVQD ILDESGASGV AVGTLRRDEG GAQRFARSVG EAFTAGIDVD WQRFFTGADS ARPVGLPTYA FQRRRYWLEA GPGGGLDAGG LGQSGVDHPL LGAVVELPGQ DGVVLTGRIC VGTHPWLADH AVGPAVVFPG TGFVELVLRA GDEVACPVLE ELTLETPLVI DGGESVQVQV AVTAAGQGGR RGVSVYARTG QQPWTRHATA TLTTTSTTDH GQWGQWPPAD AEAVDVSSHY ETLAAAGYGY GPAFQGLKRA WVRNDEVFAE VELGEREAAE ASRYGIHPAL LDACLHATGL TGERAEGVAL PFAWSGVELL ASGAQHVRVH VVPAEGGAEG GAAGIRIADG TGAPVAVVSS LVSRPLPADG LSSRARNGHE ALHRLQWVMP PSWNPPHVGG AIAVIGTVGE DDALTEALHR NGLKVRSYAD LQAASTDKDV TTLVIPCPTR QPDDQGPLED LRSELDRVVA VLGGWLADER LAGTRLVLVT TGAVTVDTGT LDAGGLVGAA VSGLVRSAQA ENPGRILLID LDHTPESLTA LATLLAVEDE PQIAIRSGRV LVPRLARADS GDDLPLPGGA GGWRLDCPAK GSIDGLKLIP APEADRDLAP GEVRVQVRAA GLNFRDVVVT LGMVPEQGGA IGGEFAGVVA EVGPEVEGLR VGDRVMGLGE GTFGPRLVAD RRMVARMPFG WGFARAASVP GAFATAWYGL VDLAGLARGE RVLIHSGAGG VGMAAIQIAR HLGAEVFATA SPGKHHILHA LGLPDDHIAS SRDLDFARTF PRVDVVLNSL AGPFTDASLN LLTEDGRFIE MGKTDRRDPD HVAATHPHAC YRSFDLMDAG HERIGQILNH LLHLFTEREL AALPVRCWPI QQARHAFRHM AQARHTGKIV LTVPHAMDRE GTVLVTGGTG GLGAILTRHL ADHHQIKHLL LASRRGEQAP NADQLRTDLE AAGASVDIVA CDLSDPSQIT TLLDRVPAEH PLTAVFHTAG VLDDGTITSL TPDKFDRVLS PKADAAWHLH HTTRHLDLAA FVLYSSSAGT LDSAGQGNYS AANAFLDALA IHRQTTGLPA QSLAWGLWHQ PSGMSAHLTT TDITRIQQAG YHPITTTQGN QLLDTALTTP QPHLLPLPIN TRTLTKRHDG IPPILRGLVH TPTRRTANTA TTATNQSALQ QHLAGLSPVE QVGELTMLVR ECAAKVIGYS EPEAIRSEQN FLEAGFDSLT AMELRNVLNK ATGLRMPATA VFDYGTAAAL ARYIADEISF DAEIARTPSS GAAGPDAGGM LASMLRDAAP AGKLREGLEM LEAAAKLRPS FRRLDDLDRS YRPLALASGP ALPKLFCFST PMALGGAAQF ARLAVHFQGV RDLYALQVPG YAPDDSLPDT VDVIVRMWAE SIQETAGDDP FVMIGYCGGG NFAHAAVTYL EQKGVRPQGL VLLDTFLPGS EVIDELGGQM LEGMFDRAET FGPFSDTRMT AMGRYYRLFR ETEVVDVETP VLFLRPDTPL PSGPDGERSR VGNWRASWHL EHDLCEVRGD HLTMLEGEAG SIAQAIEEWL KPSDGRSNGA //