ID A0A1B4Z9A6_9ACTN Unreviewed; 2480 AA. AC A0A1B4Z9A6; DT 02-NOV-2016, integrated into UniProtKB/TrEMBL. DT 02-NOV-2016, sequence version 1. DT 19-JAN-2022, entry version 24. DE SubName: Full=PKS (KS-AT-DH-ER-KR-ACP-TE) {ECO:0000313|EMBL:BAV56012.1}; GN Name=flvP3 {ECO:0000313|EMBL:BAV56012.1}; OS Actinomadura fulva subsp. indica. OC Bacteria; Actinobacteria; Streptosporangiales; Thermomonosporaceae; OC Actinomadura. OX NCBI_TaxID=1752060 {ECO:0000313|EMBL:BAV56012.1}; RN [1] {ECO:0000313|EMBL:BAV56012.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=ATCC 53714 {ECO:0000313|EMBL:BAV56012.1}; RX PubMed=26818633; DOI=10.1080/09168451.2015.1132155; RA Miyanaga A., Hayakawa Y., Numakura M., Hashimoto J., Teruya K., Hirano T., RA Shin-ya K., Kudo F., Eguchi T.; RT "Identification of the Fluvirucin B2 (Sch 38518) Biosynthetic Gene Cluster RT from Actinomadura fulva subsp. indica ATCC 53714: substrate Specificity of RT the ?-Amino Acid Selective Adenylating Enzyme FlvN."; RL Biosci. Biotechnol. Biochem. 80:935-941(2016). CC -!- FUNCTION: Fatty acid synthetase is a multifunctional enzyme that CC catalyzes the de novo biosynthesis of long-chain saturated fatty acids CC starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This CC multifunctional protein contains 7 catalytic activities and a site for CC the binding of the prosthetic group 4'-phosphopantetheine of the acyl CC carrier protein ([ACP]) domain. {ECO:0000256|ARBA:ARBA00023442}. CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-butenoyl-[ACP] + H(+) + NADPH = butanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41812, Rhea:RHEA-COMP:9627, Rhea:RHEA-COMP:9628, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78453, ChEBI:CHEBI:78454; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41813; CC Evidence={ECO:0000256|ARBA:ARBA00023357}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-decenoyl-[ACP] + H(+) + NADPH = decanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41864, Rhea:RHEA-COMP:9639, Rhea:RHEA-COMP:9640, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78467, ChEBI:CHEBI:78468; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41865; CC Evidence={ECO:0000256|ARBA:ARBA00023387}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-dodecenoyl-[ACP] + H(+) + NADPH = dodecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41880, Rhea:RHEA-COMP:9643, Rhea:RHEA- CC COMP:9644, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41881; CC Evidence={ECO:0000256|ARBA:ARBA00023385}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexadecenoyl-[ACP] + H(+) + NADPH = hexadecanoyl-[ACP] + CC NADP(+); Xref=Rhea:RHEA:41912, Rhea:RHEA-COMP:9651, Rhea:RHEA- CC COMP:9652, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78481, ChEBI:CHEBI:78483; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41913; CC Evidence={ECO:0000256|ARBA:ARBA00023345}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-hexenoyl-[ACP] + H(+) + NADPH = hexanoyl-[ACP] + NADP(+); CC Xref=Rhea:RHEA:41832, Rhea:RHEA-COMP:9631, Rhea:RHEA-COMP:9632, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78458, ChEBI:CHEBI:78459; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41833; CC Evidence={ECO:0000256|ARBA:ARBA00023359}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octadecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC octadecanoyl-[ACP]; Xref=Rhea:RHEA:41928, Rhea:RHEA-COMP:9655, CC Rhea:RHEA-COMP:9656, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78489, ChEBI:CHEBI:78495; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41929; CC Evidence={ECO:0000256|ARBA:ARBA00023376}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-octenoyl-[ACP] + H(+) + NADPH = NADP(+) + octanoyl-[ACP]; CC Xref=Rhea:RHEA:41848, Rhea:RHEA-COMP:9635, Rhea:RHEA-COMP:9636, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78462, ChEBI:CHEBI:78463; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41849; CC Evidence={ECO:0000256|ARBA:ARBA00023420}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(2E)-tetradecenoyl-[ACP] + H(+) + NADPH = NADP(+) + CC tetradecanoyl-[ACP]; Xref=Rhea:RHEA:41896, Rhea:RHEA-COMP:9647, CC Rhea:RHEA-COMP:9648, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78475, ChEBI:CHEBI:78477; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41897; CC Evidence={ECO:0000256|ARBA:ARBA00023368}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41808, Rhea:RHEA-COMP:9626, Rhea:RHEA-COMP:9627, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78451, ChEBI:CHEBI:78453; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41809; CC Evidence={ECO:0000256|ARBA:ARBA00023402}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41860, Rhea:RHEA-COMP:9638, Rhea:RHEA-COMP:9639, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78466, ChEBI:CHEBI:78467; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41861; CC Evidence={ECO:0000256|ARBA:ARBA00023388}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41876, Rhea:RHEA-COMP:9642, Rhea:RHEA-COMP:9643, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78470, ChEBI:CHEBI:78472; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41877; CC Evidence={ECO:0000256|ARBA:ARBA00023351}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41908, Rhea:RHEA-COMP:9650, Rhea:RHEA-COMP:9651, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78480, ChEBI:CHEBI:78481; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41909; CC Evidence={ECO:0000256|ARBA:ARBA00023401}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41828, Rhea:RHEA-COMP:9630, Rhea:RHEA-COMP:9631, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78457, ChEBI:CHEBI:78458; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41829; CC Evidence={ECO:0000256|ARBA:ARBA00023373}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41924, Rhea:RHEA-COMP:9654, Rhea:RHEA-COMP:9655, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78488, ChEBI:CHEBI:78489; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41925; CC Evidence={ECO:0000256|ARBA:ARBA00023399}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:41844, Rhea:RHEA-COMP:9634, Rhea:RHEA-COMP:9635, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78461, ChEBI:CHEBI:78462; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41845; CC Evidence={ECO:0000256|ARBA:ARBA00023332}; CC -!- CATALYTIC ACTIVITY: CC Reaction=(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + CC H2O; Xref=Rhea:RHEA:41892, Rhea:RHEA-COMP:9646, Rhea:RHEA-COMP:9647, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78474, ChEBI:CHEBI:78475; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41893; CC Evidence={ECO:0000256|ARBA:ARBA00023398}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxobutanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxybutanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41804, Rhea:RHEA-COMP:9625, CC Rhea:RHEA-COMP:9626, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78450, ChEBI:CHEBI:78451; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41805; CC Evidence={ECO:0000256|ARBA:ARBA00023413}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxodecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41856, Rhea:RHEA-COMP:9637, CC Rhea:RHEA-COMP:9638, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78464, ChEBI:CHEBI:78466; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41857; CC Evidence={ECO:0000256|ARBA:ARBA00023418}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxododecanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxydodecanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41872, Rhea:RHEA-COMP:9641, CC Rhea:RHEA-COMP:9642, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78469, ChEBI:CHEBI:78470; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41873; CC Evidence={ECO:0000256|ARBA:ARBA00023416}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyhexadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41904, Rhea:RHEA- CC COMP:9649, Rhea:RHEA-COMP:9650, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78478, ChEBI:CHEBI:78480; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41905; CC Evidence={ECO:0000256|ARBA:ARBA00023390}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxohexanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyhexanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41824, Rhea:RHEA-COMP:9629, CC Rhea:RHEA-COMP:9630, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78456, ChEBI:CHEBI:78457; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41825; CC Evidence={ECO:0000256|ARBA:ARBA00023346}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctadecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxyoctadecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41920, Rhea:RHEA- CC COMP:9653, Rhea:RHEA-COMP:9654, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78487, ChEBI:CHEBI:78488; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41921; CC Evidence={ECO:0000256|ARBA:ARBA00023419}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxooctanoyl-[ACP] + H(+) + NADPH = (3R)-hydroxyoctanoyl- CC [ACP] + NADP(+); Xref=Rhea:RHEA:41840, Rhea:RHEA-COMP:9633, CC Rhea:RHEA-COMP:9634, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, CC ChEBI:CHEBI:58349, ChEBI:CHEBI:78460, ChEBI:CHEBI:78461; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41841; CC Evidence={ECO:0000256|ARBA:ARBA00023364}; CC -!- CATALYTIC ACTIVITY: CC Reaction=3-oxotetradecanoyl-[ACP] + H(+) + NADPH = (3R)- CC hydroxytetradecanoyl-[ACP] + NADP(+); Xref=Rhea:RHEA:41888, CC Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9646, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:78473, CC ChEBI:CHEBI:78474; Evidence={ECO:0000256|ARBA:ARBA00023367}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41889; CC Evidence={ECO:0000256|ARBA:ARBA00023367}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41916, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9653, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78483, ChEBI:CHEBI:78487; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41917; CC Evidence={ECO:0000256|ARBA:ARBA00023365}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9632, Rhea:RHEA-COMP:9633, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78459, ChEBI:CHEBI:78460; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41837; CC Evidence={ECO:0000256|ARBA:ARBA00023361}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41852, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9636, Rhea:RHEA-COMP:9637, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78463, ChEBI:CHEBI:78464; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41853; CC Evidence={ECO:0000256|ARBA:ARBA00023414}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H(+) + malonyl-[ACP] + tetradecanoyl-[ACP] = 3- CC oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41900, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9648, Rhea:RHEA-COMP:9649, CC Rhea:RHEA-COMP:9685, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78449, ChEBI:CHEBI:78477, CC ChEBI:CHEBI:78478; Evidence={ECO:0000256|ARBA:ARBA00023341}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41901; CC Evidence={ECO:0000256|ARBA:ARBA00023341}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + hexadecanoyl-[ACP] = H(+) + hexadecanoate + holo-[ACP]; CC Xref=Rhea:RHEA:41932, Rhea:RHEA-COMP:9652, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78483; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41933; CC Evidence={ECO:0000256|ARBA:ARBA00023378}; CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + tetradecanoyl-[ACP] = H(+) + holo-[ACP] + CC tetradecanoate; Xref=Rhea:RHEA:30123, Rhea:RHEA-COMP:9648, Rhea:RHEA- CC COMP:9685, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30807, CC ChEBI:CHEBI:64479, ChEBI:CHEBI:78477; EC=3.1.2.14; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:30124; CC Evidence={ECO:0000256|ARBA:ARBA00023410}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + H(+) CC + NADPH; Xref=Rhea:RHEA:17397, Rhea:RHEA-COMP:9916, Rhea:RHEA- CC COMP:9945, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78776, ChEBI:CHEBI:78827; EC=1.1.1.100; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17399; CC Evidence={ECO:0000256|ARBA:ARBA00023333}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O; CC Xref=Rhea:RHEA:13097, Rhea:RHEA-COMP:9925, Rhea:RHEA-COMP:9945, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:78784, ChEBI:CHEBI:78827; EC=4.2.1.59; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:13098; CC Evidence={ECO:0000256|ARBA:ARBA00023394}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + CC H(+) + NADPH; Xref=Rhea:RHEA:22564, Rhea:RHEA-COMP:9925, Rhea:RHEA- CC COMP:9926, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, CC ChEBI:CHEBI:78784, ChEBI:CHEBI:78785; EC=1.3.1.39; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:22566; CC Evidence={ECO:0000256|ARBA:ARBA00023381}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:22836, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:9916, Rhea:RHEA-COMP:14125, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78776, ChEBI:CHEBI:138651; CC EC=2.3.1.41; Evidence={ECO:0000256|ARBA:ARBA00023389}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22837; CC Evidence={ECO:0000256|ARBA:ARBA00023389}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + 2n H(+) + n malonyl-CoA + 2n NADPH = a long-chain CC fatty acid + n CO2 + (n+1) CoA + 2n NADP(+); Xref=Rhea:RHEA:14993, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287, CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57384, ChEBI:CHEBI:57560, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=2.3.1.85; CC Evidence={ECO:0000256|ARBA:ARBA00023370}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14994; CC Evidence={ECO:0000256|ARBA:ARBA00023370}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-CoA + holo-[ACP] = acetyl-[ACP] + CoA; CC Xref=Rhea:RHEA:41788, Rhea:RHEA-COMP:9621, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446; EC=2.3.1.38; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41789; CC Evidence={ECO:0000256|ARBA:ARBA00023397}; CC -!- CATALYTIC ACTIVITY: CC Reaction=acetyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41800, Rhea:RHEA-COMP:9621, CC Rhea:RHEA-COMP:9623, Rhea:RHEA-COMP:9625, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78446, ChEBI:CHEBI:78449, ChEBI:CHEBI:78450; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41801; CC Evidence={ECO:0000256|ARBA:ARBA00023352}; CC -!- CATALYTIC ACTIVITY: CC Reaction=butanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CC CO2 + holo-[ACP]; Xref=Rhea:RHEA:41820, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9628, Rhea:RHEA-COMP:9629, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78454, ChEBI:CHEBI:78456; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41821; CC Evidence={ECO:0000256|ARBA:ARBA00023403}; CC -!- CATALYTIC ACTIVITY: CC Reaction=decanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxododecanoyl-[ACP] CC + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41868, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9640, Rhea:RHEA-COMP:9641, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:78449, ChEBI:CHEBI:78468, ChEBI:CHEBI:78469; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41869; CC Evidence={ECO:0000256|ARBA:ARBA00023396}; CC -!- CATALYTIC ACTIVITY: CC Reaction=dodecanoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxotetradecanoyl- CC [ACP] + CO2 + holo-[ACP]; Xref=Rhea:RHEA:41884, Rhea:RHEA-COMP:9623, CC Rhea:RHEA-COMP:9644, Rhea:RHEA-COMP:9645, Rhea:RHEA-COMP:9685, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:64479, CC ChEBI:CHEBI:65264, ChEBI:CHEBI:78449, ChEBI:CHEBI:78473; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41885; CC Evidence={ECO:0000256|ARBA:ARBA00023348}; CC -!- COFACTOR: CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942; CC Evidence={ECO:0000256|ARBA:ARBA00001957}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LC095592; BAV56012.1; -; Genomic_DNA. DR GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0008693; F:3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0047451; F:3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0004317; F:3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC. DR GO; GO:0004316; F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity; IEA:UniProtKB-EC. DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:UniProtKB-EC. DR GO; GO:0004313; F:[acyl-carrier-protein] S-acetyltransferase activity; IEA:UniProtKB-EC. DR GO; GO:0047117; F:enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; IEA:UniProtKB-EC. DR GO; GO:0016295; F:myristoyl-[acyl-carrier-protein] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0004320; F:oleoyl-[acyl-carrier-protein] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0016296; F:palmitoyl-[acyl-carrier-protein] hydrolase activity; IEA:UniProtKB-EC. DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro. DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt. DR Gene3D; 1.10.1200.10; -; 1. DR Gene3D; 3.10.129.110; -; 1. DR Gene3D; 3.40.366.10; -; 1. DR Gene3D; 3.40.47.10; -; 1. DR Gene3D; 3.40.50.1820; -; 1. DR InterPro; IPR029058; AB_hydrolase. DR InterPro; IPR001227; Ac_transferase_dom_sf. DR InterPro; IPR036736; ACP-like_sf. DR InterPro; IPR014043; Acyl_transferase. DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase. DR InterPro; IPR013154; ADH_N. DR InterPro; IPR011032; GroES-like_sf. DR InterPro; IPR018201; Ketoacyl_synth_AS. DR InterPro; IPR014031; Ketoacyl_synth_C. DR InterPro; IPR014030; Ketoacyl_synth_N. DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR032821; PKS_assoc. DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom. DR InterPro; IPR020807; PKS_dehydratase. DR InterPro; IPR042104; PKS_dehydratase_sf. DR InterPro; IPR020843; PKS_ER. DR InterPro; IPR013968; PKS_KR. DR InterPro; IPR020806; PKS_PP-bd. DR InterPro; IPR020802; PKS_thioesterase. DR InterPro; IPR015083; Polyketide_synth_docking. DR InterPro; IPR009081; PP-bd_ACP. DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS. DR InterPro; IPR001031; Thioesterase. DR InterPro; IPR016039; Thiolase-like. DR Pfam; PF00698; Acyl_transf_1; 1. DR Pfam; PF08240; ADH_N; 1. DR Pfam; PF08990; Docking; 1. DR Pfam; PF16197; KAsynt_C_assoc; 1. DR Pfam; PF00109; ketoacyl-synt; 1. DR Pfam; PF02801; Ketoacyl-synt_C; 1. DR Pfam; PF08659; KR; 1. DR Pfam; PF00550; PP-binding; 1. DR Pfam; PF14765; PS-DH; 1. DR Pfam; PF00975; Thioesterase; 1. DR SMART; SM00827; PKS_AT; 1. DR SMART; SM00826; PKS_DH; 1. DR SMART; SM00829; PKS_ER; 1. DR SMART; SM00825; PKS_KS; 1. DR SMART; SM00823; PKS_PP; 1. DR SMART; SM00824; PKS_TE; 1. DR SUPFAM; SSF50129; SSF50129; 1. DR SUPFAM; SSF51735; SSF51735; 3. DR SUPFAM; SSF52151; SSF52151; 1. DR SUPFAM; SSF53474; SSF53474; 1. DR SUPFAM; SSF53901; SSF53901; 1. DR SUPFAM; SSF55048; SSF55048; 1. DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1. DR PROSITE; PS50075; CARRIER; 1. DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1. PE 4: Predicted; KW Coiled coil {ECO:0000256|SAM:Coils}; KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516}; KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022516}; KW Lyase {ECO:0000256|ARBA:ARBA00023239}; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268}; KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450, ECO:0000256|PROSITE- KW ProRule:PRU00258}; Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898}; KW S-nitrosylation {ECO:0000256|ARBA:ARBA00022799}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}. FT DOMAIN 2103..2178 FT /note="Carrier" FT /evidence="ECO:0000259|PROSITE:PS50075" FT REGION 463..530 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COILED 11..31 FT /evidence="ECO:0000256|SAM:Coils" FT COMPBIAS 490..530 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 2138 FT /note="O-(pantetheine 4'-phosphoryl)serine" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00258" SQ SEQUENCE 2480 AA; 261632 MW; FE91EDE488158C28 CRC64; MADEEKLLAY LRRVTEDLQQ ANRRLRDVET KEREPIAIVS MACRYPGGVN SPEDLWNLVL NREQGIVPFP KDRGWDLEAL YDPDPDTSGT TYARAGGFLD GADEFDAAFF GISPREAVAM DPQQRVVLEV AWELMERAGL HPGSLRGTPT GVFIGAFAND YEVGRGPLPE GSEGYLGTGN SSSVISGRVA YTLGLEGPAV TVDTACSSSL VAIHLACQSL RNGESTLALA GGVTIMATPE LLVDFSHQRG LARDDRCKAF SDEADGTVLS EGVGVLLLER LSDALAGGRR VLGVIRGSAV NQDGASNGLT APNGPSQERV IRQALANAGV CAGEVDVVEA HGTGTALGDP IEAQALLATY GRDRDAGVPL WLGSVKSNIG HSQAAAGVAG VIKMVQAMRH GVLPQTLHVS APTSHVDWEG GGVRLLDRAR EWPRLGGRPR RAGVSSFGVS GTNAHVIVEQ APDPEPRQEQ APDAQPQQEQ APDGQPRQAF ELEPRQDVRP QEDTHEDLRE GAQEDVRAGA REDVRAGARE DVRAGAQEDL REGVRVGVPV VSGVVPWVVS GRGVAALRAQ AGRLVDFVEA RAGLPVAGVG LSLVRGRAVF EDRAVVLGGD RQELLTGLRS VAAGEPDTGP VPGPVSGVVS GVVSGSRDAV LVFPGQGAQW VGMGVELAQA SPVFAARLGE CFGEIGRWVE WDPAGVLADP AGAGLASIEV LQPVVFAVGV ALAALWESVG VRPAAVVGHS QGEVAAACVA GVLSLADAVR VVVLRSRLFA RRLQGRGAIA AVALPADVVR EEVAAWGGVL EVSADNGPTS CAVAGSLPAL EEFVERVRGR GVRARVIEAT VASHSSMVEP LRDELVGMLG GVAARQGRVP FYSTVTGGLV DGGGLGAEYW FANARRPVVF QQAVRALLAD GRTGFIEASP HPVLTMAVQD ILDESGASGV AVGTLRRDEG GAQRFARSVG EAFTAGIDVD WQRFFTGADS ARPVGLPTYA FQRRRYWLEA GPGGGLDAGG LGQSGVDHPL LGAVVELPGQ DGVVLTGRIC VGTHPWLADH AVGPAVVFPG TGFVELVLRA GDEVACPVLE ELTLETPLVI DGGESVQVQV AVTAAGQGGR RGVSVYARTG QQPWTRHATA TLTTTSTTDH GQWGQWPPAD AEAVDVSSHY ETLAAAGYGY GPAFQGLKRA WVRNDEVFAE VELGEREAAE ASRYGIHPAL LDACLHATGL TGERAEGVAL PFAWSGVELL ASGAQHVRVH VVPAEGGAEG GAAGIRIADG TGAPVAVVSS LVSRPLPADG LSSRARNGHE ALHRLQWVMP PSWNPPHVGG AIAVIGTVGE DDALTEALHR NGLKVRSYAD LQAASTDKDV TTLVIPCPTR QPDDQGPLED LRSELDRVVA VLGGWLADER LAGTRLVLVT TGAVTVDTGT LDAGGLVGAA VSGLVRSAQA ENPGRILLID LDHTPESLTA LATLLAVEDE PQIAIRSGRV LVPRLARADS GDDLPLPGGA GGWRLDCPAK GSIDGLKLIP APEADRDLAP GEVRVQVRAA GLNFRDVVVT LGMVPEQGGA IGGEFAGVVA EVGPEVEGLR VGDRVMGLGE GTFGPRLVAD RRMVARMPFG WGFARAASVP GAFATAWYGL VDLAGLARGE RVLIHSGAGG VGMAAIQIAR HLGAEVFATA SPGKHHILHA LGLPDDHIAS SRDLDFARTF PRVDVVLNSL AGPFTDASLN LLTEDGRFIE MGKTDRRDPD HVAATHPHAC YRSFDLMDAG HERIGQILNH LLHLFTEREL AALPVRCWPI QQARHAFRHM AQARHTGKIV LTVPHAMDRE GTVLVTGGTG GLGAILTRHL ADHHQIKHLL LASRRGEQAP NADQLRTDLE AAGASVDIVA CDLSDPSQIT TLLDRVPAEH PLTAVFHTAG VLDDGTITSL TPDKFDRVLS PKADAAWHLH HTTRHLDLAA FVLYSSSAGT LDSAGQGNYS AANAFLDALA IHRQTTGLPA QSLAWGLWHQ PSGMSAHLTT TDITRIQQAG YHPITTTQGN QLLDTALTTP QPHLLPLPIN TRTLTKRHDG IPPILRGLVH TPTRRTANTA TTATNQSALQ QHLAGLSPVE QVGELTMLVR ECAAKVIGYS EPEAIRSEQN FLEAGFDSLT AMELRNVLNK ATGLRMPATA VFDYGTAAAL ARYIADEISF DAEIARTPSS GAAGPDAGGM LASMLRDAAP AGKLREGLEM LEAAAKLRPS FRRLDDLDRS YRPLALASGP ALPKLFCFST PMALGGAAQF ARLAVHFQGV RDLYALQVPG YAPDDSLPDT VDVIVRMWAE SIQETAGDDP FVMIGYCGGG NFAHAAVTYL EQKGVRPQGL VLLDTFLPGS EVIDELGGQM LEGMFDRAET FGPFSDTRMT AMGRYYRLFR ETEVVDVETP VLFLRPDTPL PSGPDGERSR VGNWRASWHL EHDLCEVRGD HLTMLEGEAG SIAQAIEEWL KPSDGRSNGA //