ID   A0A182C3B3_9ARAE        Unreviewed;       967 AA.
AC   A0A182C3B3;
DT   07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT   07-SEP-2016, sequence version 1.
DT   05-FEB-2025, entry version 22.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   Name=SrPEPC {ECO:0000313|EMBL:BAV13983.1};
OS   Symplocarpus renifolius.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Araceae; Orontioideae;
OC   Symplocarpus.
OX   NCBI_TaxID=477955 {ECO:0000313|EMBL:BAV13983.1};
RN   [1] {ECO:0000313|EMBL:BAV13983.1}
RP   NUCLEOTIDE SEQUENCE.
RA   Sayed M.A., Umekawa Y., Ito K.;
RT   "Phosphoenolpyruvate carboxylase plays a major role in replenishment of the
RT   tricarboxylic acid cycle in thermogenic florets of skunk cabbage,
RT   Symplocarpus renifolius.";
RL   Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC       forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC       tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003774}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = phosphoenolpyruvate +
CC         hydrogencarbonate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|ARBA:ARBA00048995};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
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DR   EMBL; LC155943; BAV13983.1; -; mRNA.
DR   AlphaFoldDB; A0A182C3B3; -.
DR   SMR; A0A182C3B3; -.
DR   BRENDA; 4.1.1.31; 15084.
DR   GO; GO:0048046; C:apoplast; IEA:TreeGrafter.
DR   GO; GO:0009507; C:chloroplast; IEA:TreeGrafter.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0048366; P:leaf development; IEA:TreeGrafter.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   FunFam; 1.20.1440.90:FF:000001; Phosphoenolpyruvate carboxylase 1; 1.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF33; PHOSPHOENOLPYRUVATE CARBOXYLASE 3; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533};
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Pyruvate {ECO:0000313|EMBL:BAV13983.1}.
FT   ACT_SITE        174
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        602
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   967 AA;  110442 MW;  8FA0503E02076953 CRC64;
     MARNANNLEK LASIDAQLRL LAPGKVSEDD KLVEYDALLL DRFLDILQDL HGEDLKETVQ
     ECYELSAEYE GKHDPQKLEE LGNVLTSLDP GDSIVIASSF SHMLNLANLA EEVQIAYRRR
     IKLKKGDFAD ENSATTESDI EETLKRLVVQ LKKSPEEVFD ALKNQTVDLV FTAHPTQSVR
     RSLLQKHGRI RNCLAQLYAK DITPDDKQEL DEALQREIQA AFRTDEIRRT PPTPQDEMRA
     GMSYFHETIW KGVPKFLRRV DTALKNIGIN ERLPYNAPLI QFSSWMGGDR DGNPRVTPEV
     TRDVCLLARM MAANLYYSQI EDLMFELSMW RCSEELRVRA NELHRSSRKD AKHYIEFWKK
     VPSNEPYRVI LGDVRDKLYH TRERSRHLLA SGISDIPEES TFTDVEQFLE PLELCYRSLC
     ASGDRAIADG SLLDFLRQVS TFGLSLVRLD IRQESDRHTD VIDAITQHLG IGSYRDWPEE
     KRQEWLLSEL SGKRPLFGPD LPKTEEISDV LETLHVIAEL PSDNFEAYII SMATSPSDVL
     AVELLQRECH VKKPLRVVPL FEKLADLEAA PAALARLFSI DWYKNRIDGK QEVMIGYSDS
     GKDAGRFSAA WQLYKAQEEL IKVAKQYGVK LTMFHGRGGT VGRGGGPTHL AILSQPPDTI
     HGSLRVTVQG EVIEQSFGEE HLCFRTLQRF TAATLEHGMH PPVSPKPEWR ALMDEMAVVA
     TEEYRSIVFQ EPRFVEYFRL ATPETEYGRM NIGSRPSKRK PSGGIESLRA IPWIFAWTQT
     RFHLPVWLGF GAAFKHILEK DIRNFHMLQE MYNEWPFFRV TIDLVEMVFA KGDPGIAALY
     DKLLVSDDLW SFGERLRTNY EETKHLLLQV AGHKHLLEEN PYLKQRLRLR HSYITTLNVC
     QACTLKRIRD PSFHVKVRPH LSKEIVDSSK SAAELVKLNP TSEYAPGLED TLILTMKGIA
     AGMQNTG
//