ID A0A179R2G9_PSEPU Unreviewed; 473 AA. AC A0A179R2G9; DT 07-SEP-2016, integrated into UniProtKB/TrEMBL. DT 07-SEP-2016, sequence version 1. DT 12-AUG-2020, entry version 12. DE RecName: Full=DNA ligase (NAD(+)) {ECO:0000256|ARBA:ARBA00012722}; DE EC=6.5.1.2 {ECO:0000256|ARBA:ARBA00012722}; DE Flags: Fragment; GN ORFNames=AYO08_16955 {ECO:0000313|EMBL:OAS02822.1}; OS Pseudomonas putida (Arthrobacter siderocapsulatus). OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OX NCBI_TaxID=303 {ECO:0000313|EMBL:OAS02822.1, ECO:0000313|Proteomes:UP000078222}; RN [1] {ECO:0000313|EMBL:OAS02822.1, ECO:0000313|Proteomes:UP000078222} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=INSali382 {ECO:0000313|EMBL:OAS02822.1, RC ECO:0000313|Proteomes:UP000078222}; RA Jones-Dias D., Manageiro V., Ferreira E., Canica M.; RT "Antimicrobial susceptibility and integron diversity in organic and RT conventionally grown fruits and vegetables."; RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- CC (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta- CC nicotinamide D-nucleotide.; EC=6.5.1.2; CC Evidence={ECO:0000256|ARBA:ARBA00000066}; CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:OAS02822.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LSUZ01000209; OAS02822.1; -; Genomic_DNA. DR EnsemblBacteria; OAS02822; OAS02822; AYO08_16955. DR Proteomes; UP000078222; Unassembled WGS sequence. DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW. DR GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-EC. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW. DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW. DR HAMAP; MF_01587; DNA_ligase_B; 1. DR InterPro; IPR020923; DNA_ligase_B. DR InterPro; IPR033136; DNA_ligase_CS. DR InterPro; IPR013839; DNAligase_adenylation. DR InterPro; IPR013840; DNAligase_N. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR004150; NAD_DNA_ligase_OB. DR InterPro; IPR010994; RuvA_2-like. DR Pfam; PF01653; DNA_ligase_aden; 1. DR Pfam; PF03120; DNA_ligase_OB; 1. DR SMART; SM00532; LIGANc; 1. DR SUPFAM; SSF47781; SSF47781; 1. DR SUPFAM; SSF50249; SSF50249; 1. DR PROSITE; PS01056; DNA_LIGASE_N2; 1. PE 3: Inferred from homology; KW Dioxygenase {ECO:0000313|EMBL:OAS02822.1}; KW DNA damage {ECO:0000256|ARBA:ARBA00022763}; KW DNA repair {ECO:0000256|ARBA:ARBA00023204}; KW DNA replication {ECO:0000256|ARBA:ARBA00022705}; KW Ligase {ECO:0000256|ARBA:ARBA00022598}; KW NAD {ECO:0000256|ARBA:ARBA00023027}; KW Oxidoreductase {ECO:0000313|EMBL:OAS02822.1}. FT DOMAIN 1..334 FT /note="LIGANc" FT /evidence="ECO:0000259|SMART:SM00532" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:OAS02822.1" SQ SEQUENCE 473 AA; 52336 MW; 26CEAD1F228703BF CRC64; HPVPHTGVDK LADRQAVARW MAGKTGVWVQ PKVDGVAVSL TYQQGRLVQL TSRGDGVHGH DWSRHIPQLG AVTRQLPEAV DLHLQGELYL RLDEHVQAKA GSANARGTVA GLLARKQLTG AQGNAIGLFV WGWPHGPEQQ AERLAQLARL GFPDSQLYSI AIDTLEDAAH WREHWYRSAL PFATDGVILR QGSRPPAERW QAKAPYWIAA WKYPYAQALA EVRDVRFRVG RTGRVTPVLH LQPVTLDDRR ITQVSLGSLA RWQRLDIRPG DQVAVSLAGL TIPRLEHVVH RAVERQPITA PAPDQHHAHS CWQASEGCDE QFIARLTWLG GKQGLALPRT GPGTWRRLVE AGLVTSMTDW LQLDAERLQQ APGISRLTAA HLMGSFDEAR SRPFDQWLRA LGMPIGKHLP LTGDWQALAS RSAGQWQTVP GIGAKRSRQL VEFFAASEVQ AIAAQLAEAG IEGFRTPPQR IEQ //