ID A0A159B7D6_9CAUD Unreviewed; 302 AA. AC A0A159B7D6; DT 06-JUL-2016, integrated into UniProtKB/TrEMBL. DT 06-JUL-2016, sequence version 1. DT 12-AUG-2020, entry version 13. DE RecName: Full=Helix-destabilizing protein {ECO:0000256|ARBA:ARBA00018858}; DE AltName: Full=Single-stranded DNA-binding protein {ECO:0000256|ARBA:ARBA00018590}; GN ORFNames=HY03_0245 {ECO:0000313|EMBL:AKJ72887.1}; OS Escherichia phage HY03. OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus; OC Escherichia virus HY03. OX NCBI_TaxID=1654926 {ECO:0000313|EMBL:AKJ72887.1, ECO:0000313|Proteomes:UP000204314}; RN [1] {ECO:0000313|EMBL:AKJ72887.1, ECO:0000313|Proteomes:UP000204314} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RA Lee J.-H., Park E.-A., Lee D.-H.; RT "Complete Genome Sequence of E. coli O157:H7 Bacteriophage HY03."; RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Binds preferentially to single-stranded DNA and therefore, CC destabilizes double-stranded DNA. It is involved in DNA replication, CC repair and recombination. Binds ss-DNA as the replication fork advances CC and stimulates the replisome processivity and accuracy. CC {ECO:0000256|ARBA:ARBA00001987}. CC -!- SUBUNIT: Homodimer in the absence of DNA, monomer when binding DNA. CC {ECO:0000256|ARBA:ARBA00011770}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KR269718; AKJ72887.1; -; Genomic_DNA. DR RefSeq; YP_009284218.1; NC_031047.1. DR GeneID; 29079086; -. DR KEGG; vg:29079086; -. DR Proteomes; UP000204314; Genome. DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR012339; Phage_T4_Gp32_ssDNA-bd. DR Pfam; PF08804; gp32; 1. DR SUPFAM; SSF50249; SSF50249; 1. PE 4: Predicted; KW DNA damage {ECO:0000256|ARBA:ARBA00022763}; KW DNA recombination {ECO:0000256|ARBA:ARBA00023172}; KW DNA repair {ECO:0000256|ARBA:ARBA00023204}; KW DNA replication {ECO:0000256|ARBA:ARBA00022705}; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000313|EMBL:AKJ72887.1}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}. FT DOMAIN 40..240 FT /note="gp32" FT /evidence="ECO:0000259|Pfam:PF08804" FT REGION 275..302 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 278..293 FT /note="Polar" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 302 AA; 33509 MW; 53AC1C17C8921788 CRC64; MFKRKSTAEL AAQMAKLAGN KGGFSSEDKG EWKLKLDNAG NGQAVIRFLP SKNDEQAPFA ILVNHGFKKN GKWYIETCSS THGDYDSCPV CQYISKNDLY NTDNKEYSLV KRKTSYWANI LVVKDPAAPE NEGKVFKYRF GKKIWDKINA MIAVDVEMGE TPVDVTCPWE GANFVLKVKQ VSGFSNYDES KFLNQSAIPN IDDESFQKEL FEQMVDLSEM TSKDKFKSFE ELSTKFSQVM GTAAMGGAAA TAAKKADKVA DDLDAFNVDD FKTKTEDDFM SSSSGSSSSA DDTDLDDLLN DL //