ID A0A140D6E3_9ENTR Unreviewed; 290 AA. AC A0A140D6E3; DT 11-MAY-2016, integrated into UniProtKB/TrEMBL. DT 11-MAY-2016, sequence version 1. DT 05-DEC-2018, entry version 9. DE RecName: Full=4-hydroxybenzoate octaprenyltransferase {ECO:0000256|HAMAP-Rule:MF_01635, ECO:0000256|SAAS:SAAS01075854}; DE EC=2.5.1.- {ECO:0000256|HAMAP-Rule:MF_01635, ECO:0000256|SAAS:SAAS00336940}; DE AltName: Full=4-HB polyprenyltransferase {ECO:0000256|HAMAP-Rule:MF_01635}; GN Name=hdbP {ECO:0000313|EMBL:AMK07429.1}; GN Synonyms=ubiA {ECO:0000256|HAMAP-Rule:MF_01635}; OS Klebsiella sp. NT18-21. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Klebsiella. OX NCBI_TaxID=1778872 {ECO:0000313|EMBL:AMK07429.1}; RN [1] {ECO:0000313|EMBL:AMK07429.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=NT18-21Contig1 {ECO:0000313|EMBL:AMK07429.1}; RX PubMed=26822785; DOI=10.1186/s12864-016-2404-0; RA Maruthamuthu M., Jimenez D.J., Stevens P., van Elsas J.D.; RT "A multi-substrate approach for functional metagenomics-based RT screening for (hemi)cellulases in two wheat straw-degrading microbial RT consortia unveils novel thermoalkaliphilic enzymes."; RL BMC Genomics 17:86-86(2016). CC -!- FUNCTION: Catalyzes the prenylation of para-hydroxybenzoate (PHB) CC with an all-trans polyprenyl group. Mediates the second step in CC the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, CC which is the condensation of the polyisoprenoid side chain with CC PHB, generating the first membrane-bound Q intermediate 3- CC octaprenyl-4-hydroxybenzoate. {ECO:0000256|HAMAP-Rule:MF_01635, CC ECO:0000256|SAAS:SAAS01075872}. CC -!- CATALYTIC ACTIVITY: CC Reaction=4-hydroxybenzoate + all-trans-octaprenyl diphosphate = 4- CC hydroxy-3-all-trans-octaprenylbenzoate + diphosphate; CC Xref=Rhea:RHEA:27782, ChEBI:CHEBI:1617, ChEBI:CHEBI:17879, CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57711; EC=2.5.1.39; CC Evidence={ECO:0000256|HAMAP-Rule:MF_01635, CC ECO:0000256|SAAS:SAAS01075846}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP- CC Rule:MF_01635, ECO:0000256|SAAS:SAAS01075861}; CC -!- PATHWAY: Cofactor biosynthesis; ubiquinone biosynthesis. CC {ECO:0000256|HAMAP-Rule:MF_01635, ECO:0000256|SAAS:SAAS01075851}. CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP- CC Rule:MF_01635, ECO:0000256|SAAS:SAAS01075873}; Multi-pass membrane CC protein {ECO:0000256|HAMAP-Rule:MF_01635, CC ECO:0000256|SAAS:SAAS01075873}. CC -!- SIMILARITY: Belongs to the UbiA prenyltransferase family. CC {ECO:0000256|HAMAP-Rule:MF_01635, ECO:0000256|SAAS:SAAS01075853}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KU505137; AMK07429.1; -; Genomic_DNA. DR UniPathway; UPA00232; -. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0008412; F:4-hydroxybenzoate octaprenyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006744; P:ubiquinone biosynthetic process; IEA:UniProtKB-UniRule. DR HAMAP; MF_01635; UbiA; 1. DR InterPro; IPR006370; HB_polyprenyltransferase-like. DR InterPro; IPR039653; Prenyltransferase. DR InterPro; IPR000537; UbiA_prenyltransferase. DR InterPro; IPR030470; UbiA_prenylTrfase_CS. DR PANTHER; PTHR11048; PTHR11048; 1. DR Pfam; PF01040; UbiA; 1. DR TIGRFAMs; TIGR01474; ubiA_proteo; 1. DR PROSITE; PS00943; UBIA; 1. PE 3: Inferred from homology; KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS01075859}; KW Cell membrane {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS01075855}; KW Magnesium {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS01075847}; KW Membrane {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS00415574}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS00415579, ECO:0000313|EMBL:AMK07429.1}; KW Transmembrane {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS00415577}; KW Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS00415562}; KW Ubiquinone biosynthesis {ECO:0000256|HAMAP-Rule:MF_01635, KW ECO:0000256|SAAS:SAAS01075866}. FT TRANSMEM 21 40 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. FT TRANSMEM 46 70 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. FT TRANSMEM 99 132 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. FT TRANSMEM 152 182 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. FT TRANSMEM 213 233 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. FT TRANSMEM 239 257 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. FT TRANSMEM 269 288 Helical. {ECO:0000256|HAMAP-Rule: FT MF_01635}. SQ SEQUENCE 290 AA; 32602 MW; 7BCEEDE3DB05DCED CRC64; MERSLRPNKL LAYHQLMRTD KPIGALLLLW PTLWALWVAA PGVPPLWILA VFVAGVWLMR AAGCVVNDYA DRKFDGHVKR TANRPLPSGA VSEKEARILF AVLVLLSFLL VLTLNTMTIL LSVAGLALAW VYPFMKRYTH LPQVVLGAAF GWSIPMAFAA VSESLPLSCW LMLLANILWA VAYDTQYAMV DRDDDVKIGI KSTAILFGEN DRLIIGILQA CVLVLMAVIG WLNGLHWEFY WSILIAGALF VYQQKLIRNR ERGPCFQAFL NNNYVGLVLF IGLAMSYWQF //