ID A0A127EJZ2_CLOPF Unreviewed; 347 AA. AC A0A127EJZ2; DT 08-JUN-2016, integrated into UniProtKB/TrEMBL. DT 08-JUN-2016, sequence version 1. DT 06-JUL-2016, entry version 2. DE RecName: Full=Ribosomal RNA large subunit methyltransferase N {ECO:0000256|SAAS:SAAS00536183}; DE EC=2.1.1.- {ECO:0000256|SAAS:SAAS00297775}; DE EC=2.1.1.192 {ECO:0000256|SAAS:SAAS00536141}; GN ORFNames=JFP838_10925 {ECO:0000313|EMBL:AMN36255.1}; OS Clostridium perfringens. OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. OX NCBI_TaxID=1502 {ECO:0000313|EMBL:AMN36255.1, ECO:0000313|Proteomes:UP000070260}; RN [1] {ECO:0000313|EMBL:AMN36255.1, ECO:0000313|Proteomes:UP000070260} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=JP838 {ECO:0000313|EMBL:AMN36255.1, RC ECO:0000313|Proteomes:UP000070260}; RX PubMed=26859667; RA Mehdizadeh Gohari I., Kropinski A.M., Weese S.J., Parreira V.R., RA Whitehead A.E., Boerlin P., Prescott J.F.; RT "Plasmid Characterization and Chromosome Analysis of Two netF+ RT Clostridium perfringens Isolates Associated with Foal and Canine RT Necrotizing Enteritis."; RL PLoS ONE 11:E0148344-E0148344(2016). CC -!- FUNCTION: Specifically methylates position 2 of adenine 2503 in CC 23S rRNA and position 2 of adenine 37 in tRNAs. CC {ECO:0000256|SAAS:SAAS00536188}. CC -!- CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + adenine(2503) in CC 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L- CC homocysteine + L-methionine + 5'-deoxyadenosine + 2- CC methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin. CC {ECO:0000256|SAAS:SAAS00536154}. CC -!- CATALYTIC ACTIVITY: 2 S-adenosyl-L-methionine + adenine(37) in CC tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + CC L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA + 2 CC oxidized [2Fe-2S] ferredoxin. {ECO:0000256|SAAS:SAAS00536172}. CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Evidence={ECO:0000256|SAAS:SAAS00611820}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|SAAS:SAAS00297762}. CC -!- SIMILARITY: Belongs to the radical SAM superfamily. RlmN family. CC {ECO:0000256|SAAS:SAAS00571858}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP010994; AMN36255.1; -; Genomic_DNA. DR RefSeq; WP_061428803.1; NZ_CP010994.1. DR Proteomes; UP000070260; Chromosome. DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW. DR Gene3D; 3.20.20.70; -; 1. DR HAMAP; MF_01849; RNA_methyltr_RlmN; 1. DR InterPro; IPR013785; Aldolase_TIM. DR InterPro; IPR006638; Elp3/MiaB/NifB. DR InterPro; IPR027492; RNA_MTrfase_RlmN. DR InterPro; IPR004383; rRNA_lsu_MTrfase_RlmN/Cfr. DR InterPro; IPR007197; rSAM. DR PANTHER; PTHR30544; PTHR30544; 1. DR Pfam; PF04055; Radical_SAM; 1. DR PIRSF; PIRSF006004; CHP00048; 1. DR SMART; SM00729; Elp3; 1. DR TIGRFAMs; TIGR00048; rRNA_mod_RlmN; 1. PE 3: Inferred from homology; KW 4Fe-4S {ECO:0000256|SAAS:SAAS00463035}; KW Complete proteome {ECO:0000313|Proteomes:UP000070260}; KW Cytoplasm {ECO:0000256|SAAS:SAAS00462865}; KW Disulfide bond {ECO:0000256|SAAS:SAAS00571810}; KW Iron {ECO:0000256|SAAS:SAAS00462960}; KW Iron-sulfur {ECO:0000256|SAAS:SAAS00462907}; KW Metal-binding {ECO:0000256|SAAS:SAAS00462842}; KW Methyltransferase {ECO:0000256|SAAS:SAAS00462825, KW ECO:0000313|EMBL:AMN36255.1}; KW rRNA processing {ECO:0000256|SAAS:SAAS00536180}; KW S-adenosyl-L-methionine {ECO:0000256|SAAS:SAAS00462941}; KW Transferase {ECO:0000256|SAAS:SAAS00463018, KW ECO:0000313|EMBL:AMN36255.1}; KW tRNA processing {ECO:0000256|SAAS:SAAS00536152}. SQ SEQUENCE 347 AA; 39191 MW; 1DDDD6A82A4BD5B0 CRC64; MKNILDFTLE ELKEWMKENG ESAFRAKQIF DWIYKKEVFN FEEMKNISKA LIGKLSENFY IGIPEVIDYL SSNEDGTRKI LLGLGDGNII ECVIMRYKYG NSICVSTQIG CRMGCKFCAS TLEGMVRNLT AGEILSEVLI GQKLLGERIS NIVLMGSGEP LDNYDNVMKF LELVNADYGL NIGQRHITLS TCGLVPKIRE MADKEMQVTL AISLHAVSDE KRKTIMPIAN KYSISEILDA CNYYIEKTGR RITFEYSLVS GVNDTKEDAK SLGRLLKGML CHVNLIPVNE IKENEFKKST KKDIETFLNT LKTYGVEATV RREMGSDINA ACGQLRRSYI KSKGLKL //