ID A0A125S6H1_9FUNG Unreviewed; 352 AA. AC A0A125S6H1; DT 13-APR-2016, integrated into UniProtKB/TrEMBL. DT 13-APR-2016, sequence version 1. DT 24-JUL-2024, entry version 39. DE RecName: Full=Methionine aminopeptidase {ECO:0000256|RuleBase:RU003653}; DE EC=3.4.11.18 {ECO:0000256|RuleBase:RU003653}; OS Actinomucor elegans. OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; OC Mucoromycetes; Mucorales; Mucorineae; Mucoraceae; Actinomucor. OX NCBI_TaxID=64647 {ECO:0000313|EMBL:AME15507.1}; RN [1] {ECO:0000313|EMBL:AME15507.1} RP NUCLEOTIDE SEQUENCE. RA Murphy D.; RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Cotranslationally removes the N-terminal methionine from CC nascent proteins. The N-terminal methionine is often cleaved when the CC second residue in the primary sequence is small and uncharged (Met- CC Ala-, Cys, Gly, Pro, Ser, Thr, or Val). {ECO:0000256|HAMAP- CC Rule:MF_03174, ECO:0000256|RuleBase:RU003653}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Release of N-terminal amino acids, preferentially methionine, CC from peptides and arylamides.; EC=3.4.11.18; CC Evidence={ECO:0000256|HAMAP-Rule:MF_03174, CC ECO:0000256|RuleBase:RU003653}; CC -!- COFACTOR: CC Name=Co(2+); Xref=ChEBI:CHEBI:48828; CC Evidence={ECO:0000256|RuleBase:RU003653}; CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|RuleBase:RU003653}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|RuleBase:RU003653}; CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033; CC Evidence={ECO:0000256|RuleBase:RU003653}; CC Note=Binds 2 divalent metal cations per subunit. Has a high-affinity CC and a low affinity metal-binding site. The true nature of the CC physiological cofactor is under debate. The enzyme is active with CC cobalt, zinc, manganese or divalent iron ions. CC {ECO:0000256|RuleBase:RU003653}; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Evidence={ECO:0000256|HAMAP-Rule:MF_03174}; CC Name=Co(2+); Xref=ChEBI:CHEBI:48828; CC Evidence={ECO:0000256|HAMAP-Rule:MF_03174}; CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; CC Evidence={ECO:0000256|HAMAP-Rule:MF_03174}; CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033; CC Evidence={ECO:0000256|HAMAP-Rule:MF_03174}; CC Note=Binds 2 divalent metal cations per subunit. Has a high-affinity CC and a low affinity metal-binding site. The true nature of the CC physiological cofactor is under debate. The enzyme is active with zinc, CC cobalt, manganese or divalent iron ions. Has high activity with zinc; CC zinc cofactor is transferred into the active site region by the ZNG1 CC zinc chaperone. {ECO:0000256|HAMAP-Rule:MF_03174}; CC -!- SUBUNIT: Associates with the 60S ribosomal subunit of the 80S CC translational complex. {ECO:0000256|HAMAP-Rule:MF_03174}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03174}. CC -!- SIMILARITY: Belongs to the peptidase M24A family. Methionine CC aminopeptidase type 1 subfamily. {ECO:0000256|HAMAP-Rule:MF_03174, CC ECO:0000256|PROSITE-ProRule:PRU01357}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KP973584; AME15507.1; -; mRNA. DR AlphaFoldDB; A0A125S6H1; -. DR GO; GO:0022626; C:cytosolic ribosome; IEA:UniProtKB-UniRule. DR GO; GO:0004239; F:initiator methionyl aminopeptidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule. DR GO; GO:0070006; F:metalloaminopeptidase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW. DR CDD; cd01086; MetAP1; 1. DR Gene3D; 3.90.230.10; Creatinase/methionine aminopeptidase superfamily; 1. DR HAMAP; MF_01974; MetAP_1; 1. DR InterPro; IPR036005; Creatinase/aminopeptidase-like. DR InterPro; IPR000994; Pept_M24. DR InterPro; IPR001714; Pept_M24_MAP. DR InterPro; IPR002467; Pept_M24A_MAP1. DR InterPro; IPR031615; Zfn-C6H2. DR NCBIfam; TIGR00500; met_pdase_I; 1. DR PANTHER; PTHR43330; METHIONINE AMINOPEPTIDASE; 1. DR PANTHER; PTHR43330:SF7; METHIONINE AMINOPEPTIDASE 1; 1. DR Pfam; PF00557; Peptidase_M24; 1. DR Pfam; PF15801; zf-C6H2; 1. DR PRINTS; PR00599; MAPEPTIDASE. DR SUPFAM; SSF55920; Creatinase/aminopeptidase; 1. DR PROSITE; PS00680; MAP_1; 1. DR PROSITE; PS52013; ZF_C6H2; 1. PE 2: Evidence at transcript level; KW Aminopeptidase {ECO:0000256|ARBA:ARBA00022438, ECO:0000256|HAMAP- KW Rule:MF_03174}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03174}; KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_03174}; KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_03174, KW ECO:0000256|RuleBase:RU003653}; KW Protease {ECO:0000256|HAMAP-Rule:MF_03174, ECO:0000256|RuleBase:RU003653}; KW Zinc {ECO:0000256|ARBA:ARBA00022833}; KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE- KW ProRule:PRU01357}. FT DOMAIN 4..58 FT /note="C6H2-type" FT /evidence="ECO:0000259|PROSITE:PS52013" FT BINDING 183 FT /ligand="a protein" FT /ligand_id="ChEBI:CHEBI:16541" FT /ligand_part="N-terminal L-methionine residue" FT /ligand_part_id="ChEBI:CHEBI:64731" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 200 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="3" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 211 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="4" FT /ligand_note="catalytic" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 211 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="3" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 274 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="4" FT /ligand_note="catalytic" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 281 FT /ligand="a protein" FT /ligand_id="ChEBI:CHEBI:16541" FT /ligand_part="N-terminal L-methionine residue" FT /ligand_part_id="ChEBI:CHEBI:64731" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 307 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="4" FT /ligand_note="catalytic" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 338 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="4" FT /ligand_note="catalytic" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" FT BINDING 338 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT /ligand_label="3" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03174" SQ SEQUENCE 352 AA; 38978 MW; 796F3C95E8BF159C CRC64; MLEKPLCASI ECKNKAHLQC PTCVKLGKNG ESFFCSQDCF KKSWGSHKAV HGDTKSPYDP FKTFKYAGPL RAVYPLSPRR TVPEEISRPD YAESGISRSE LMSRGSDIKV LTPEEIEGAK KACAITREVL ELAAKSIRVG MTTDELDRIV HEATIERGAY PSPLNYNYFP KSCCTSLNEV ICHGIPDQRP LADGDILNID ISCFYQGFHG DTNGTYLVGN VDEAGQKLVN VTRECLEKAI AAVKPGMRYR DFGKIIEDHA TKNGFSVVRA FVGHGIHQLF HCAPNVPHYA NNKAIGVCKP GHIFTIEPMI CEGVHQELMW PDGWTATTKD GKRSAQFEHT LLVTETGVEI LT //