ID A0A0S7XSX7_9BACT Unreviewed; 405 AA. AC A0A0S7XSX7; DT 17-FEB-2016, integrated into UniProtKB/TrEMBL. DT 17-FEB-2016, sequence version 1. DT 06-JUL-2016, entry version 6. DE RecName: Full=Homoserine dehydrogenase {ECO:0000256|RuleBase:RU000579}; DE EC=1.1.1.3 {ECO:0000256|RuleBase:RU000579}; GN ORFNames=AMJ44_10540 {ECO:0000313|EMBL:KPJ65331.1}; OS candidate division WOR_1 bacterium DG_54_3. OC Bacteria; candidate division WOR-1. OX NCBI_TaxID=1703775 {ECO:0000313|EMBL:KPJ65331.1, ECO:0000313|Proteomes:UP000051861}; RN [1] {ECO:0000313|EMBL:KPJ65331.1, ECO:0000313|Proteomes:UP000051861} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DG_54_3 {ECO:0000313|EMBL:KPJ65331.1}; RX PubMed=25922666; DOI=10.1186/s40168-015-0077-6; RA Baker B.J., Lazar C.S., Teske A.P., Dick G.J.; RT "Genomic resolution of linkages in carbon, nitrogen, and sulfur RT cycling among widespread estuary sediment bacteria."; RL Microbiome 3:14-14(2015). CC -!- CATALYTIC ACTIVITY: L-homoserine + NAD(P)(+) = L-aspartate 4- CC semialdehyde + NAD(P)H. {ECO:0000256|RuleBase:RU000579}. CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; L-homoserine from L-aspartate: step 3/3. CC {ECO:0000256|RuleBase:RU000579}. CC -!- PATHWAY: Amino-acid biosynthesis; L-threonine biosynthesis; L- CC threonine from L-aspartate: step 3/5. CC {ECO:0000256|RuleBase:RU000579}. CC -!- SIMILARITY: Belongs to the homoserine dehydrogenase family. CC {ECO:0000256|RuleBase:RU004171}. CC -!- CAUTION: The sequence shown here is derived from an CC EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is CC preliminary data. {ECO:0000313|EMBL:KPJ65331.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LIZX01000124; KPJ65331.1; -; Genomic_DNA. DR UniPathway; UPA00050; UER00063. DR UniPathway; UPA00051; UER00465. DR Proteomes; UP000051861; Unassembled WGS sequence. DR GO; GO:0016597; F:amino acid binding; IEA:InterPro. DR GO; GO:0004412; F:homoserine dehydrogenase activity; IEA:UniProtKB-EC. DR GO; GO:0050661; F:NADP binding; IEA:InterPro. DR GO; GO:0009097; P:isoleucine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009088; P:threonine biosynthetic process; IEA:UniProtKB-UniPathway. DR Gene3D; 3.40.50.720; -; 1. DR InterPro; IPR002912; ACT_dom. DR InterPro; IPR005106; Asp/hSer_DH_NAD-bd. DR InterPro; IPR016204; HDH. DR InterPro; IPR001342; HDH_cat. DR InterPro; IPR019811; HDH_CS. DR InterPro; IPR016040; NAD(P)-bd_dom. DR Pfam; PF01842; ACT; 1. DR Pfam; PF00742; Homoserine_dh; 1. DR Pfam; PF03447; NAD_binding_3; 1. DR PIRSF; PIRSF000098; Homoser_dehydrog; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR PROSITE; PS51671; ACT; 1. DR PROSITE; PS01042; HOMOSER_DHGENASE; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis {ECO:0000256|RuleBase:RU000579}; KW Branched-chain amino acid biosynthesis KW {ECO:0000256|RuleBase:RU000579}; KW Complete proteome {ECO:0000313|Proteomes:UP000051861}; KW Isoleucine biosynthesis {ECO:0000256|RuleBase:RU000579}; KW Methionine biosynthesis {ECO:0000256|RuleBase:RU000579}; KW NADP {ECO:0000256|PIRSR:PIRSR000098-2, ECO:0000256|RuleBase:RU000579}; KW Oxidoreductase {ECO:0000256|RuleBase:RU000579}; KW Reference proteome {ECO:0000313|Proteomes:UP000051861}; KW Threonine biosynthesis {ECO:0000256|RuleBase:RU000579}. FT DOMAIN 329 403 ACT. {ECO:0000259|PROSITE:PS51671}. FT NP_BIND 9 16 NADP. {ECO:0000256|PIRSR:PIRSR000098-2}. FT ACT_SITE 190 190 Proton donor. {ECO:0000256|PIRSR: FT PIRSR000098-1}. FT BINDING 90 90 NADP. {ECO:0000256|PIRSR:PIRSR000098-2}. FT BINDING 175 175 Substrate. {ECO:0000256|PIRSR: FT PIRSR000098-2}. SQ SEQUENCE 405 AA; 43643 MW; 703586D42715E53A CRC64; MGKIKIGILG LGVVGSAVAR NVKYVPEIEI VRGCDIRRVR VPCKLTRDPY KIINDPEIAI VVEAIGGINP AKKYVLAAIR SGKHVVTSNK ELVALHLDEL MTMAKKKGVS LLFEGSVGGG IPILSTLRDS LSANKIAEIY GIVNGTTNYI LSKMSEEGMA FSEALKKARE KGFAEADPRS DVEGYDAFYK AAILASVAFR AKVRAADVYR EGIEKITAED ILYAGEIGYV IKLLAIAKMI EGKLDVRVHP ALVSKQHPLA AVSENYNAIY VKGDPVGELM FYGQGAGGGP TASAIVSDIV QVSKGGGGKP ARLRRIKTRK IEDIESRYYI RLQAPDRFGV LAGISKAFAR KKVSIAAVVQ KETVGNVATI VILLHSVAEK NLKAALKIIE KLPVVRKVSN VIRIV //