ID A0A0R2B502_9LACO Unreviewed; 80 AA. AC A0A0R2B502; DT 20-JAN-2016, integrated into UniProtKB/TrEMBL. DT 20-JAN-2016, sequence version 1. DT 03-MAY-2023, entry version 31. DE RecName: Full=NH(3)-dependent NAD(+) synthetase {ECO:0000256|RuleBase:RU003812}; DE EC=6.3.1.5 {ECO:0000256|RuleBase:RU003812}; GN ORFNames=FC35_GL000959 {ECO:0000313|EMBL:KRM73778.1}; OS Limosilactobacillus coleohominis DSM 14060. OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; OC Limosilactobacillus. OX NCBI_TaxID=887896 {ECO:0000313|EMBL:KRM73778.1, ECO:0000313|Proteomes:UP000051013}; RN [1] {ECO:0000313|EMBL:KRM73778.1, ECO:0000313|Proteomes:UP000051013} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 14060 {ECO:0000313|EMBL:KRM73778.1, RC ECO:0000313|Proteomes:UP000051013}; RX PubMed=26415554; DOI=10.1038/ncomms9322; RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X., RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E., RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J., RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E., RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y., RA Zhang H., O'Toole P.W.; RT "Expanding the biotechnology potential of lactobacilli through comparative RT genomics of 213 strains and associated genera."; RL Nat. Commun. 6:8322-8322(2015). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + CC NAD(+); Xref=Rhea:RHEA:21188, ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, CC ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.1.5; CC Evidence={ECO:0000256|RuleBase:RU003812}; CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from CC deamido-NAD(+) (ammonia route): step 1/1. CC {ECO:0000256|RuleBase:RU004252}. CC -!- SIMILARITY: Belongs to the NAD synthetase family. CC {ECO:0000256|RuleBase:RU003811}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KRM73778.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AZEW01000353; KRM73778.1; -; Genomic_DNA. DR AlphaFoldDB; A0A0R2B502; -. DR PATRIC; fig|887896.3.peg.1247; -. DR UniPathway; UPA00253; UER00333. DR Proteomes; UP000051013; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:InterPro. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004359; F:glutaminase activity; IEA:InterPro. DR GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IEA:InterPro. DR GO; GO:0008795; F:NAD+ synthase activity; IEA:UniProtKB-EC. DR GO; GO:0009435; P:NAD biosynthetic process; IEA:UniProtKB-UniPathway. DR Gene3D; 3.40.50.620; HUPs; 1. DR InterPro; IPR022310; NAD/GMP_synthase. DR InterPro; IPR003694; NAD_synthase. DR InterPro; IPR014729; Rossmann-like_a/b/a_fold. DR Pfam; PF02540; NAD_synthase; 1. DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1. DR TIGRFAMs; TIGR00552; nadE; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU003811}; KW Ligase {ECO:0000256|RuleBase:RU003811}; KW NAD {ECO:0000256|RuleBase:RU003811}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, KW ECO:0000256|RuleBase:RU003811}; KW Reference proteome {ECO:0000313|Proteomes:UP000051013}. FT DOMAIN 2..70 FT /note="NAD/GMP synthase" FT /evidence="ECO:0000259|Pfam:PF02540" FT REGION 1..30 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 80 AA; 9233 MW; B8D8464B2565D51E CRC64; MEYLGAPAHL YEKTPTADLE EDRPALPDEQ ALGVSYHEID DYLEGKEVPA TAAQTIEGWF TKTRHKRHLP VSPQDEWWKK //