ID A0A0P0XLG1_ORYSJ Unreviewed; 975 AA. AC A0A0P0XLG1; DT 20-JAN-2016, integrated into UniProtKB/TrEMBL. DT 20-JAN-2016, sequence version 1. DT 12-AUG-2020, entry version 28. DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305}; DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305}; GN OrderedLocusNames=Os09g0315700 {ECO:0000313|EMBL:BAT07416.1}; GN ORFNames=OSNPB_090315700 {ECO:0000313|EMBL:BAT07416.1}; OS Oryza sativa subsp. japonica (Rice). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa. OX NCBI_TaxID=39947 {ECO:0000313|EMBL:BAT07416.1, ECO:0000313|Proteomes:UP000059680}; RN [1] {ECO:0000313|Proteomes:UP000059680} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nipponbare {ECO:0000313|Proteomes:UP000059680}; RX PubMed=16100779; DOI=10.1038/nature03895; RG International rice genome sequencing project (IRGSP); RT "The map-based sequence of the rice genome."; RL Nature 436:793-800(2005). RN [2] {ECO:0000313|EMBL:BAT07416.1, ECO:0000313|Proteomes:UP000059680} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Nipponbare {ECO:0000313|Proteomes:UP000059680}; RX PubMed=24280374; DOI=10.1186/1939-8433-6-4; RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R., RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L., RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H., RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.; RT "Improvement of the Oryza sativa Nipponbare reference genome using next RT generation sequence and optical map data."; RL Rice 6:4-4(2013). CC -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it CC forms oxaloacetate, a four-carbon dicarboxylic acid source for the CC tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003774}. CC -!- CATALYTIC ACTIVITY: CC Reaction=oxaloacetate + phosphate = hydrogencarbonate + CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452, CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31; CC Evidence={ECO:0000256|ARBA:ARBA00001071}; CC -!- COFACTOR: CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; CC Evidence={ECO:0000256|ARBA:ARBA00001946}; CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}. CC -!- SIMILARITY: Belongs to the PEPCase type 1 family. CC {ECO:0000256|ARBA:ARBA00008346}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AP014965; BAT07416.1; -; Genomic_DNA. DR RefSeq; XP_015611998.1; XM_015756512.1. DR SMR; A0A0P0XLG1; -. DR STRING; 4530.OS09T0315700-01; -. DR PaxDb; A0A0P0XLG1; -. DR EnsemblPlants; Os09t0315700-01; Os09t0315700-01; Os09g0315700. DR GeneID; 4346699; -. DR Gramene; Os09t0315700-01; Os09t0315700-01; Os09g0315700. DR KEGG; osa:4346699; -. DR eggNOG; ENOG502QPVS; Eukaryota. DR KO; K01595; -. DR OMA; DMYKQWP; -. DR OrthoDB; 775417at2759; -. DR Proteomes; UP000059680; Chromosome 9. DR GO; GO:0048046; C:apoplast; IBA:GO_Central. DR GO; GO:0009507; C:chloroplast; IBA:GO_Central. DR GO; GO:0005829; C:cytosol; IBA:GO_Central. DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central. DR GO; GO:0015977; P:carbon fixation; IEA:InterPro. DR GO; GO:0048366; P:leaf development; IBA:GO_Central. DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro. DR HAMAP; MF_00595; PEPcase_type1; 1. DR InterPro; IPR021135; PEP_COase. DR InterPro; IPR022805; PEP_COase_bac/pln-type. DR InterPro; IPR018129; PEP_COase_Lys_AS. DR InterPro; IPR033129; PEPCASE_His_AS. DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom. DR PANTHER; PTHR30523; PTHR30523; 1. DR Pfam; PF00311; PEPcase; 1. DR PRINTS; PR00150; PEPCARBXLASE. DR SUPFAM; SSF51621; SSF51621; 1. DR PROSITE; PS00781; PEPCASE_1; 1. DR PROSITE; PS00393; PEPCASE_2; 1. PE 3: Inferred from homology; KW Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533}; KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300}; KW Lyase {ECO:0000256|ARBA:ARBA00023239}; KW Magnesium {ECO:0000256|ARBA:ARBA00022842}; KW Reference proteome {ECO:0000313|Proteomes:UP000059680}. FT ACT_SITE 182 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10111" FT ACT_SITE 610 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10112" SQ SEQUENCE 975 AA; 110474 MW; 0CD48D5B14AA8471 CRC64; MSAMSAAGGG GGVGKVERLS SIDAQLRQLV PAKLSEDDKL IEYDALLLDR FLDVLHGLHG DDLKDLVQEC YEVAAEYETK HDVQKLDELG NMITSLDPGD SIVIAKAFSH MLNLANLAEE VQIAYRRRIK LKKGDFADEN SAMTESDIEE TLKRLVFDLK KSPAEVFDAL KSQTVDLVLT AHPTQSVRRS LLQKHSRIRN CLVQLYSKDI TPDDKQELDE ALQREIQAAF RTDEIRRTQP TPQDEMRAGM SYFHETIWKG VPKFLRRVDT ALKNIGIDER VPYNAPLIQF SSWMGGDRDG NPRVTPEVTR DVCLLARMMA ANLYCSQIED LMFELSMWRC NEELRSRADD LHRSSKKDAK HYIEFWKKVP LNEPYRVILS DVRDKLYNTR ERSRELLSSG YCDIPEEATL TNVEQLLEPL ELCYRSLCAC GDRAIADGSL LDFLRQVSTF GLSLVRLDIR QESDRHTDVL DAITTYLGIG SYREWSEERR QEWLLSELNG KRPLFGPDLP RTDEVADVLD TFHVIAELPA DSFGAYVISM ATAPSDVLAV ELLQRECHVK TPLRVVPLFE KLADLESAPA ALTRLFSISW YRQRINGKQE VMIGYSDSGK DAGRLSAAWQ MYKAQEQLVK VAKDFGVKLT MFHGRGGTVG RGGGPTHLAI LSQPPDTING SLRVTVQGEV IEQSFGEEHL CFRTLQRFTA ATLEHGMHPP SAPKPEWRAL LDEMAVVATK EYRSVVFQEP RFVEYFRLAT PETEYGRMNI GSRPSKRKPS GGIESLRAIP WIFAWTQTRF HLPVWLGFGA AFKHALQKDI RNLHMLQEMY NEWPFFRVTL DLIEMVFAKG NPGIAALYDK LLVSEDLQPL GEKLRANYVE TQKLLLQVAG HRDLLEGDPY LKQRLRLRDA YITTLNVCQA YTLKRIRDPD YHVTLRPHLS KEVMDGSKPA AELVKLNPGS EYAPGLEDTL ILTMKGIAAG LQNTG //