ID A0A0N9LME3_BETVU Unreviewed; 1365 AA. AC A0A0N9LME3; DT 20-JAN-2016, integrated into UniProtKB/TrEMBL. DT 20-JAN-2016, sequence version 1. DT 13-FEB-2019, entry version 18. DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324}; DE EC=2.7.7.6 {ECO:0000256|HAMAP-Rule:MF_01324}; DE AltName: Full=PEP {ECO:0000256|HAMAP-Rule:MF_01324}; DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324}; DE Short=RNA polymerase subunit beta'' {ECO:0000256|HAMAP-Rule:MF_01324}; GN Name=rpoC2 {ECO:0000256|HAMAP-Rule:MF_01324, GN ECO:0000313|EMBL:ALG63279.1}; OS Beta vulgaris subsp. vulgaris. OG Plastid; Chloroplast {ECO:0000313|EMBL:ALG63279.1}. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; Caryophyllales; Chenopodiaceae; Betoideae; Beta. OX NCBI_TaxID=3555 {ECO:0000313|EMBL:ALG63279.1}; RN [1] {ECO:0000313|EMBL:ALG63279.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Leaf {ECO:0000313|EMBL:ALG63279.1}; RX PubMed=26377912; DOI=10.1186/s12859-015-0726-6; RA Stadermann K.B., Weisshaar B., Holtgrawe D.; RT "SMRT sequencing only de novo assembly of the sugar beet (Beta RT vulgaris) chloroplast genome."; RL BMC Bioinformatics 16:295-295(2015). CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription CC of DNA into RNA using the four ribonucleoside triphosphates as CC substrates. {ECO:0000256|HAMAP-Rule:MF_01324}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- CC COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, CC ChEBI:CHEBI:83400; EC=2.7.7.6; Evidence={ECO:0000256|HAMAP- CC Rule:MF_01324}; CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core CC is composed of four subunits: alpha, beta, beta', and beta''. When CC a (nuclear-encoded) sigma factor is associated with the core the CC holoenzyme is formed, which can initiate transcription. CC {ECO:0000256|HAMAP-Rule:MF_01324}. CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000256|HAMAP- CC Rule:MF_01324}. CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. CC RpoC2 subfamily. {ECO:0000256|HAMAP-Rule:MF_01324}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KR230391; ALG63279.1; -; Genomic_DNA. DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule. DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule. DR Gene3D; 1.10.132.30; -; 1. DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1. DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp. DR InterPro; IPR007083; RNA_pol_Rpb1_4. DR InterPro; IPR007081; RNA_pol_Rpb1_5. DR InterPro; IPR038120; Rpb1_funnel_sf. DR Pfam; PF05000; RNA_pol_Rpb1_4; 1. DR Pfam; PF04998; RNA_pol_Rpb1_5; 2. DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1. PE 3: Inferred from homology; KW Chloroplast {ECO:0000313|EMBL:ALG63279.1}; KW DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_01324}; KW Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_01324}; KW Plastid {ECO:0000313|EMBL:ALG63279.1}; KW Transcription {ECO:0000256|HAMAP-Rule:MF_01324}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_01324}. FT DOMAIN 95 157 RNA_pol_Rpb1_4. {ECO:0000259|Pfam: FT PF05000}. FT DOMAIN 172 364 RNA_pol_Rpb1_5. {ECO:0000259|Pfam: FT PF04998}. FT DOMAIN 1176 1263 RNA_pol_Rpb1_5. {ECO:0000259|Pfam: FT PF04998}. SQ SEQUENCE 1365 AA; 155236 MW; 8F2AD5DD50B24094 CRC64; MAERANLVFH NKAIDGTAMK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID DLLTIPSKGW LVQDAEQQSL ILEKHHHYGN VHAVEKLRQS IEIWYSTSEY LRQEMNPNFR MTDPYNPVHI MSFSGARGNV SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTIRGISVSP RNGMIPERIW IQTLIGRVLA DDIYIGSRCI ATRNQDIGVG LVNRFITLRA QPISIRTPFT CRSASWICRL CYGRSPTHGD LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE HVRAPSNGKI QFNEDLVHPT RTRHGHPAFL CYIDLYVTIE SEDILHNVNI PPKSFLLVQN DQYVESEQVI AEIRAGTSTL NFKERVRKHI YSDAEGEMHW STDVYHAPEF TYGNVHLLPK TSHLWILSGR PYRSSVVPFS LSKDQDQMNT HSLSFDQIYI SNPSVTNDQV KEKLSDSFSK KEDRILNYSE LNRIGHYTLI YSAKNSDLLA KKRRNRFIIP FQSSQEREKE LMSPSGISIE IPINGIFRRN SIFAYYDDPR YRRKSSGITK YGTIEMHSIV KKEDLIEYRG VKEFRPKYQM KVDRFFFIPE EVHILPGSSS IMVRNNSIIG VDTQITLNTR SRVGGVVRVE RKKKKIELKI FSGDIHFPGE TDKISRHSGI LIPPSRTNSK DSKNLKNWIY VQRITPTKKK YFVLVRPVVP YEITDGINLT TLFPQDLLQE RDNVQLRVVN YILYGNGKAT RGISDTSIQL VRTCLVLNWN QDQKSSSMEE ARASFVEVRT NGMIRDFLRI DLVKSAISYI GKRNDPSLEK NEGSDHTNMN PFYSIYSKTK PQESFNKNQG TVRTLLGINK ECPFFIILSS SNCFRIGPFI SKGVKYHKES IKKDPLSPIR NSLGPLGTAL QIANFFSFYH LITHNQILVT NYLQLDNLKQ TFQLLKFQYY LMDENGRIYN PDPCSNIILN PFKLNWYFLH YNFCEETSTK IYLGQFVCEN VCITKNGPHL KSGQVLIVQS DYVVIRSAKP YLATPGATVH GHYGEIIYEG DTLVTFIYEK SRSGDITQGL PKVEQVLEVR SINSISMNLK KRIDGWNERI TRILGIPWGF FIGAELTIAQ SRISLVNKIQ KVYRSQGVQI HNRHIEIIVR QITSKVLVSE DGMSNVFLPG ELIGLFRAER TGRALEEAIC YRATLLGITR ASLNTQSFIS EASFQETARV LAKAALRGRI DWLKGLKENV VLGGMIPVGT GFKGFVHHSS QHKGIPLKTK KKNLFEGEMG DILFYHRELF DSFLSKNFCD RSKQQ //