ID A0A0N7AWQ4_GAMHO Unreviewed; 461 AA. AC A0A0N7AWQ4; DT 20-JAN-2016, integrated into UniProtKB/TrEMBL. DT 20-JAN-2016, sequence version 1. DT 13-FEB-2019, entry version 12. DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4 {ECO:0000256|RuleBase:RU003297}; DE EC=7.1.1.2 {ECO:0000256|RuleBase:RU003297}; GN Name=ND4 {ECO:0000313|EMBL:AJW75217.1}; OS Gambusia holbrooki (Eastern mosquitofish). OG Mitochondrion {ECO:0000313|EMBL:AJW75217.1}. OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata; OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Poeciliidae; OC Poeciliinae; Gambusia. OX NCBI_TaxID=37273 {ECO:0000313|EMBL:AJW75217.1}; RN [1] {ECO:0000313|EMBL:AJW75217.1} RP NUCLEOTIDE SEQUENCE. RA Renshaw M.A., Olds B.P., Li Y., Pfrender M.E., Lodge D.M.; RT "Introduction of universal fish and amphibian primers for long-range RT PCR amplification and whole mitochondrial genome assembly using next- RT generation sequencing techniques."; RL Submitted (OCT-2014) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory CC chain NADH dehydrogenase (Complex I) that is believed to belong to CC the minimal assembly required for catalysis. Complex I functions CC in the transfer of electrons from NADH to the respiratory chain. CC The immediate electron acceptor for the enzyme is believed to be CC ubiquinone. {ECO:0000256|RuleBase:RU003297}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, CC Rhea:RHEA-COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, CC ChEBI:CHEBI:17976, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; CC EC=7.1.1.2; Evidence={ECO:0000256|RuleBase:RU003297}; CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane CC {ECO:0000256|RuleBase:RU003297}; Multi-pass membrane protein CC {ECO:0000256|RuleBase:RU003297}. CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. CC {ECO:0000256|RuleBase:RU003297, ECO:0000256|SAAS:SAAS00707377}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KP013085; AJW75217.1; -; Genomic_DNA. DR EMBL; KP013115; AJW75607.1; -; Genomic_DNA. DR RefSeq; YP_009177952.1; NC_028274.1. DR GeneID; 26128449; -. DR CTD; 4538; -. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell. DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW. DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-UniRule. DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro. DR InterPro; IPR000260; NADH4_N. DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4. DR InterPro; IPR003918; NADH_UbQ_OxRdtase. DR InterPro; IPR001750; ND/Mrp_mem. DR PANTHER; PTHR43507; PTHR43507; 1. DR Pfam; PF01059; Oxidored_q5_N; 1. DR Pfam; PF00361; Proton_antipo_M; 1. DR PRINTS; PR01437; NUOXDRDTASE4. DR TIGRFAMs; TIGR01972; NDH_I_M; 1. PE 3: Inferred from homology; KW Electron transport {ECO:0000256|RuleBase:RU003297}; KW Membrane {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00707373}; KW Mitochondrion {ECO:0000256|RuleBase:RU003297, KW ECO:0000313|EMBL:AJW75217.1}; KW NAD {ECO:0000256|RuleBase:RU003297, ECO:0000256|SAAS:SAAS00707374}; KW Respiratory chain {ECO:0000256|RuleBase:RU003297}; KW Translocase {ECO:0000256|SAAS:SAAS01135314}; KW Transmembrane {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00707385}; KW Transmembrane helix {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00707361}; KW Transport {ECO:0000256|RuleBase:RU003297}; KW Ubiquinone {ECO:0000256|RuleBase:RU003297, KW ECO:0000256|SAAS:SAAS00699082}. FT TRANSMEM 21 42 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 62 81 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 93 111 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 117 137 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 149 168 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 195 217 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 229 252 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 258 278 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 285 304 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 310 331 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 352 370 Helical. {ECO:0000256|RuleBase:RU003297}. FT TRANSMEM 390 413 Helical. {ECO:0000256|RuleBase:RU003297}. FT DOMAIN 1 110 Oxidored_q5_N. {ECO:0000259|Pfam: FT PF01059}. FT DOMAIN 113 403 Proton_antipo_M. {ECO:0000259|Pfam: FT PF00361}. SQ SEQUENCE 461 AA; 51441 MW; AB3401EF82371944 CRC64; MLKILLPSIL LLPSTWLMPK KHLWSTTLSY SLMIAGTSLA WLALPSENGW SFLNCFMASD PISTPLLVLT CWLLPLMILA SQNHLMLEPT NRQRTFISLL ISLQIFLILA FSATELILFY IMFEATLIPT LLIITRWGNQ TERLNAGTYF LFYTLAGSLP LLIALLMMQN STGTLSFLTL PYLSSIPTHD WAHKFWWTAC LLAFLVKMPL YGAHLWLPKA HVEAPIAGSM VLAAVLLKLG GYGMIRILVI FAPSTKELSY PFIILALWGV VMTGSICLRQ TDLKALIAYS SVSHMGLVAA AILIQTPWAF TGALVLMIAH GLTSSALFCL ANTNYERSHS RTMLLVRGLQ TLLPLTALWW FTATLANLAL PPLPNLMGEL MIITSLFNWS MWTLVLTGLG MTITAGYSLY MFLTSQRGPL PSHLISWDPS HSREHLLLTM HLVPLLLLIL KPELLWGWNA C //