ID A0A0M4LT88_9HYPH Unreviewed; 261 AA. AC A0A0M4LT88; DT 09-DEC-2015, integrated into UniProtKB/TrEMBL. DT 09-DEC-2015, sequence version 1. DT 29-SEP-2021, entry version 30. DE RecName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ {ECO:0000256|RuleBase:RU362024}; DE EC=2.1.1.200 {ECO:0000256|RuleBase:RU362024}; DE AltName: Full=tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase {ECO:0000256|RuleBase:RU362024}; DE AltName: Full=tRNA Cm32/Um32 methyltransferase {ECO:0000256|RuleBase:RU362024}; GN Name=trmJ {ECO:0000256|RuleBase:RU362024}; GN ORFNames=PU02_0958 {ECO:0000313|EMBL:ALE03772.1}; OS Bartonella ancashensis. OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; OC Bartonellaceae; Bartonella. OX NCBI_TaxID=1318743 {ECO:0000313|EMBL:ALE03772.1, ECO:0000313|Proteomes:UP000057213}; RN [1] {ECO:0000313|EMBL:ALE03772.1, ECO:0000313|Proteomes:UP000057213} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=20.00 {ECO:0000313|EMBL:ALE03772.1, RC ECO:0000313|Proteomes:UP000057213}; RX PubMed=26543106; RA Hang J., Mullins K.E., Clifford R.J., Onmus-Leone F., Yang Y., Jiang J., RA Leguia M., Kasper M.R., Maguina C., Lesho E.P., Jarman R.G., Richards A.L., RA Blazes D.; RT "Complete Genome Sequence of Bartonella ancashensis Strain 20.00, Isolated RT from the Blood of a Patient with Verruga Peruana."; RL Genome Announc. 3:e01217-e01215(2015). CC -!- FUNCTION: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or CC 2'O-methylated uridine (Um32) at position 32 in tRNA. CC {ECO:0000256|RuleBase:RU362024}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-adenosyl-L-methionine + uridine(32) in tRNA = 2'-O- CC methyluridine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42936, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10290, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.200; CC Evidence={ECO:0000256|RuleBase:RU362024}; CC -!- CATALYTIC ACTIVITY: CC Reaction=cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O- CC methylcytidine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42932, Rhea:RHEA-COMP:10288, Rhea:RHEA-COMP:10289, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=2.1.1.200; CC Evidence={ECO:0000256|RuleBase:RU362024}; CC -!- SUBUNIT: Homodimer. {ECO:0000256|RuleBase:RU362024}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU362024}. CC -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase CC superfamily. RNA methyltransferase TrmH family. CC {ECO:0000256|ARBA:ARBA00007228}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP010401; ALE03772.1; -; Genomic_DNA. DR EnsemblBacteria; ALE03772; ALE03772; PU02_0958. DR KEGG; banc:PU02_0958; -. DR PATRIC; fig|1318743.3.peg.972; -. DR Proteomes; UP000057213; Chromosome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro. DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. DR Gene3D; 3.40.1280.10; -; 1. DR InterPro; IPR029028; Alpha/beta_knot_MTases. DR InterPro; IPR004384; RNA_MeTrfase_TrmJ/LasT. DR InterPro; IPR001537; SpoU_MeTrfase. DR InterPro; IPR029026; tRNA_m1G_MTases_N. DR PANTHER; PTHR42786; PTHR42786; 1. DR Pfam; PF00588; SpoU_methylase; 1. DR PIRSF; PIRSF004808; LasT; 1. DR SUPFAM; SSF75217; SSF75217; 1. DR TIGRFAMs; TIGR00050; rRNA_methyl_1; 1. PE 3: Inferred from homology; KW Cytoplasm {ECO:0000256|RuleBase:RU362024}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, KW ECO:0000256|RuleBase:RU362024}; KW Reference proteome {ECO:0000313|Proteomes:UP000057213}; KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU362024}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ALE03772.1}; KW tRNA processing {ECO:0000256|RuleBase:RU362024}. FT DOMAIN 5..155 FT /note="SpoU_methylase" FT /evidence="ECO:0000259|Pfam:PF00588" SQ SEQUENCE 261 AA; 29450 MW; A69A81AFEF2F766C CRC64; MDGPTIILVE PQLPENIGMV ARAMANFGLF KLRLVNPREK FPNEKARAAA SKADHVINNT QVFCKLCDAI ADLNYVIGTT ARKRYSFKTV KSAVEATRTL RHHENIGHKT GILFGRERWG LENDEISLVD EIVTFPVNPA FASLNIAQAV LIMSYEWMKS GLENTDDTAF RAEEIKPVDK ATLHGFLSQL EEALDIRGYF RPKERKEVMI TNIRSVFTQA HFGESEVRLL RGIISSLDHF SPKFPRGSGA PIGRDRKKCE Y //