ID A0A0M3TC14_9ACTN Unreviewed; 858 AA. AC A0A0M3TC14; DT 09-DEC-2015, integrated into UniProtKB/TrEMBL. DT 09-DEC-2015, sequence version 1. DT 28-JUN-2023, entry version 40. DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808}; DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808}; GN ORFNames=AL705_02270 {ECO:0000313|EMBL:ALE19594.1}; OS Lawsonella clevelandensis. OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Lawsonellaceae; OC Lawsonella. OX NCBI_TaxID=1528099 {ECO:0000313|EMBL:ALE19594.1, ECO:0000313|Proteomes:UP000068137}; RN [1] {ECO:0000313|EMBL:ALE19594.1, ECO:0000313|Proteomes:UP000068137} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=X1698 {ECO:0000313|EMBL:ALE19594.1, RC ECO:0000313|Proteomes:UP000068137}; RX PubMed=26659691; RA Nicholson A.C., Bell M., Humrighouse B.W., McQuiston J.R.; RT "Complete Genome Sequences for Two Strains of a Novel Fastidious, Partially RT Acid-Fast, Gram-Positive Corynebacterineae Bacterium, Derived from Human RT Clinical Samples."; RL Genome Announc. 3:e01462-15(2015). CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4; CC Evidence={ECO:0000256|ARBA:ARBA00034618}; CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily. CC {ECO:0000256|ARBA:ARBA00009922}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP012390; ALE19594.1; -; Genomic_DNA. DR AlphaFoldDB; A0A0M3TC14; -. DR STRING; 1528099.AL705_02270; -. DR EnsemblBacteria; ALE19594; ALE19594; AL705_02270. DR KEGG; cbq:AL705_02270; -. DR PATRIC; fig|1562462.4.peg.468; -. DR Proteomes; UP000068137; Chromosome. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro. DR CDD; cd17932; DEXQc_UvrD; 1. DR CDD; cd18807; SF1_C_UvrD; 2. DR Gene3D; 1.10.10.160; -; 1. DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2. DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf. DR InterPro; IPR014017; DNA_helicase_UvrD-like_C. DR InterPro; IPR000212; DNA_helicase_UvrD/REP. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR014016; UvrD-like_ATP-bd. DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1. DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1. DR Pfam; PF00580; UvrD-helicase; 1. DR Pfam; PF13361; UvrD_C; 1. DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1. DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1. DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE- KW ProRule:PRU00560}; DNA-binding {ECO:0000256|ARBA:ARBA00023125}; KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE- KW ProRule:PRU00560}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE- KW ProRule:PRU00560}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE- KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000068137}. FT DOMAIN 31..318 FT /note="UvrD-like helicase ATP-binding" FT /evidence="ECO:0000259|PROSITE:PS51198" FT DOMAIN 319..637 FT /note="UvrD-like helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51217" FT REGION 1..21 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 729..803 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT BINDING 52..59 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560" SQ SEQUENCE 858 AA; 93843 MW; BB0ECB58F60660EB CRC64; MPVSGKASTS RTARAGWHRR RDPHAAEQLL EGLNPQQQQA VQHTGTPLLI VAGAGSGKTT VLTRRIAYVL ETQPVRASQI LAITFTNKAA AEMRERVQHI VGTEATYMWV STFHSMCVRI LRAQAAQLEV VNSNFTIYDA DDAKRLLTMI VKDLNLDPKK YAPKALANRI SNWKNELIDA ETAAADAHEG HFDKVASEVY GIYQARLRAA NAFDFDDLIG ETVALLESHP AIAQYYQRRF RHVLVDEYQD TNHAQYMLVR ALVGDGSNGV EPAELCVVGD ADQSIYAFRG ATIRNIVEFE RDFTNATTIL LEQNYRSTQN ILSAANAVID NNAGRRKKNL WTAAGDGPLI DLYVADNEHD EARFIGSEID KLHDEGMSYS DMAVFYRTNN GSRPLEDAFI RTGLPYKVVG GTRFYERMEV RDIVAYLKVV ENPADAVSLR RIINTPRRGI GDRAMSCLAM FAEQQGVSLW EACCRAQNGT VPFLNSRAEN AIAAFVSLIT GIQNHSAGAE HTAGADSLPD LGELVELVID RSGYRASLEG SNDPQDQARL DNLNELVSVA HEFSADAASL RALEESFAQD DTNGISAETV AEGTEGLAAP GSLAAFLERV SLTSDTDQIP DEDDGMITLM TLHSAKGLEF PVVFVTGWED GMFPHMRALA DENELSEERR LAYVGITRAK RKLYVTRAVV RSSWGQPQSN PQSRFLDEIP AHLLNVLREA PEFDDDDWSY GSSGHSWGTG GGSPWGAGSR SGGTGSREGK SSAHHVGRSG ISRSGRYGDV HKRWGNPVET RRMAPQIANP RHSSAISVEK GDRVVHDKYG LGKVLEVTGV GASATALVDF GSSGVVRLML IAAPIEKL //