ID A0A0M2AAW4_ENTFL Unreviewed; 642 AA. AC A0A0M2AAW4; DT 11-NOV-2015, integrated into UniProtKB/TrEMBL. DT 11-NOV-2015, sequence version 1. DT 08-NOV-2023, entry version 31. DE SubName: Full=PTS system, sucrose-specific IIBC component {ECO:0000313|EMBL:EOI97083.1}; GN ORFNames=UMC_01556 {ECO:0000313|EMBL:EOI97083.1}; OS Enterococcus faecalis EnGen0302. OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae; OC Enterococcus. OX NCBI_TaxID=1158660 {ECO:0000313|EMBL:EOI97083.1, ECO:0000313|Proteomes:UP000013839}; RN [1] {ECO:0000313|EMBL:EOI97083.1, ECO:0000313|Proteomes:UP000013839} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=CH116 {ECO:0000313|EMBL:EOI97083.1, RC ECO:0000313|Proteomes:UP000013839}; RG The Broad Institute Genome Sequencing Platform; RG The Broad Institute Genome Sequencing Center for Infectious Disease; RA Earl A.M., Gilmore M.S., Lebreton F., Walker B., Young S.K., Zeng Q., RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L., RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A., RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C., RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.; RT "The Genome Sequence of Enterococcus faecalis CH116."; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:EOI97083.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AJEG01000010; EOI97083.1; -; Genomic_DNA. DR RefSeq; WP_002360285.1; NZ_KB947273.1. DR AlphaFoldDB; A0A0M2AAW4; -. DR EnsemblBacteria; EOI97083; EOI97083; UMC_01556. DR GeneID; 60893903; -. DR PATRIC; fig|1158660.3.peg.1525; -. DR HOGENOM; CLU_012312_2_1_9; -. DR Proteomes; UP000013839; Unassembled WGS sequence. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW. DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW. DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro. DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW. DR CDD; cd00212; PTS_IIB_glc; 1. DR Gene3D; 2.70.70.10; Glucose Permease (Domain IIA); 1. DR Gene3D; 3.30.1360.60; Glucose permease domain IIB; 1. DR InterPro; IPR011055; Dup_hybrid_motif. DR InterPro; IPR036878; Glu_permease_IIB. DR InterPro; IPR018113; PTrfase_EIIB_Cys. DR InterPro; IPR001127; PTS_EIIA_1_perm. DR InterPro; IPR003352; PTS_EIIC. DR InterPro; IPR013013; PTS_EIIC_1. DR InterPro; IPR001996; PTS_IIB_1. DR InterPro; IPR010973; PTS_IIBC_sucr. DR NCBIfam; TIGR00826; EIIB_glc; 1. DR NCBIfam; TIGR00830; PTBA; 1. DR NCBIfam; TIGR01996; PTS-II-BC-sucr; 1. DR PANTHER; PTHR30175:SF7; NEGATIVE REGULATOR OF SACY ACTIVITY; 1. DR PANTHER; PTHR30175; PHOSPHOTRANSFERASE SYSTEM TRANSPORT PROTEIN; 1. DR Pfam; PF00358; PTS_EIIA_1; 1. DR Pfam; PF00367; PTS_EIIB; 1. DR Pfam; PF02378; PTS_EIIC; 1. DR SUPFAM; SSF51261; Duplicated hybrid motif; 1. DR SUPFAM; SSF55604; Glucose permease domain IIB; 1. DR PROSITE; PS51093; PTS_EIIA_TYPE_1; 1. DR PROSITE; PS00371; PTS_EIIA_TYPE_1_HIS; 1. DR PROSITE; PS51098; PTS_EIIB_TYPE_1; 1. DR PROSITE; PS01035; PTS_EIIB_TYPE_1_CYS; 1. DR PROSITE; PS51103; PTS_EIIC_TYPE_1; 1. PE 4: Predicted; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Kinase {ECO:0000256|ARBA:ARBA00022777}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683}; KW Sugar transport {ECO:0000256|ARBA:ARBA00022597}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}. FT TRANSMEM 112..133 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 153..173 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 185..203 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 215..241 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 262..281 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 293..317 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 329..348 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 368..390 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 397..416 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 436..462 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 5..88 FT /note="PTS EIIB type-1" FT /evidence="ECO:0000259|PROSITE:PS51098" FT DOMAIN 120..478 FT /note="PTS EIIC type-1" FT /evidence="ECO:0000259|PROSITE:PS51103" FT DOMAIN 508..612 FT /note="PTS EIIA type-1" FT /evidence="ECO:0000259|PROSITE:PS51093" FT ACT_SITE 27 FT /note="Phosphocysteine intermediate; for EIIB activity" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00421" SQ SEQUENCE 642 AA; 69478 MW; D4D08B8E47A909AD CRC64; MAENYQQAAK DIIQLIGMDN IISVTHCQTR LRFILKDHEQ VDGKQLEKID LVKGVFYNGG QYQVILGTGI VTKVYDEIEK LGINVVSKAE QTAILKNNET GMRKTMRILS EIFIPIVPVI AATGLFLGLK GVIFNDTFLQ LFGASVANIP ESFQQIVSVI TDTVFAFLPA LIVWSTFKAF NATPVIGIVI GLMMVSPILP NAYAVATPDS GVKAIMAFGF IPVVGAQGSV LSAIAAGIIG AKIELFFRKK MPNILDQIFT PFMTMLITFL IMILGIGPIL HTVELGMVDV VQWLIGLPLG LGGFVIGASY PLMVLIGIHH TLTMVETSLL ANTGFNALIT ICAMYGFANV GSCLAFAKKA QDSKVKSTAI GSMLSQLFGV SEPVLFGLLI RWNLKPLLCV LFTSGLGGAI LAIFHIQSNS YGLAVIPSFL MYIYSAHQLV IYLLVALLSV GVCYALTSLF AIPQEVLISD KVIEEEEREV FEMQHNTLDE QLFSPVTGYA MNLTAVNDPV FSSEMMGKGL AIMPTANKVY APADGLLNLV AETGHAYGIQ TDAGAEVLIH IGIDTVTLGQ EVFQTQVTQG HRVKKGDLLG TFDRKAIKEA GLDSTVMVII TNTSSYLSVE PMMSDHNEIT PEQIILNLNT PN //