ID A0A0L7KB96_PLAFX Unreviewed; 293 AA. AC A0A0L7KB96; DT 11-NOV-2015, integrated into UniProtKB/TrEMBL. DT 11-NOV-2015, sequence version 1. DT 22-FEB-2023, entry version 31. DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; DE EC=2.1.1.33 {ECO:0000256|HAMAP-Rule:MF_03055}; DE AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; DE AltName: Full=tRNA(m7G46)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; GN ORFNames=PFHG_01892 {ECO:0000313|EMBL:KOB60199.1}; OS Plasmodium falciparum (isolate HB3). OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; OC Plasmodiidae; Plasmodium; Plasmodium (Laverania). OX NCBI_TaxID=137071 {ECO:0000313|EMBL:KOB60199.1, ECO:0000313|Proteomes:UP000054289}; RN [1] {ECO:0000313|EMBL:KOB60199.1, ECO:0000313|Proteomes:UP000054289} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HB3 {ECO:0000313|EMBL:KOB60199.1}; RG The Broad Institute Genome Sequencing Platform; RA Volkman S.K., Neafsey D.E., Dash A.P., Chitnis C.E., Hartl D.L., RA Young S.K., Zeng Q., Koehrsen M., Alvarado L., Berlin A., Borenstein D., RA Chapman S.B., Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., RA Griggs A., Gujja S., Heilman E.R., Heiman D.I., Howarth C., Jen D., RA Larson L., Mehta T., Neiman D., Park D., Pearson M., Roberts A., Saif S., RA Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., RA Yandava C., Haas B., Henn M.R., Nusbaum C., Birren B.; RT "Annotation of Plasmodium falciparum HB3."; RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Proteomes:UP000054289} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RG The Broad Institute Genome Sequencing Platform; RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Henn M., Jaffe D., RA Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E., RA Brockman W., MacCallum I.A., Rounsley S., Young S., LaButti K., RA Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R., RA Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J., RA Kodira C., Zeng Q., Oleary S., Yandava C., Alvarado L., Wirth D., RA Volkman S., Hartl D.; RT "The genome sequence of the Plasmodium falciparum HB3."; RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at position 46 CC (m7G46) in tRNA. {ECO:0000256|HAMAP-Rule:MF_03055}. CC -!- CATALYTIC ACTIVITY: CC Reaction=guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)- CC methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:42708, Rhea:RHEA-COMP:10188, Rhea:RHEA-COMP:10189, CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, CC ChEBI:CHEBI:74480; EC=2.1.1.33; Evidence={ECO:0000256|HAMAP- CC Rule:MF_03055}; CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. CC {ECO:0000256|HAMAP-Rule:MF_03055}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|HAMAP-Rule:MF_03055}. CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase CC superfamily. TrmB family. {ECO:0000256|HAMAP-Rule:MF_03055}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CH671956; KOB60199.1; -; Genomic_DNA. DR AlphaFoldDB; A0A0L7KB96; -. DR SMR; A0A0L7KB96; -. DR EnsemblProtists; KOB60199; KOB60199; PFHG_01892. DR VEuPathDB; PlasmoDB:PfHB3_110030700; -. DR UniPathway; UPA00989; -. DR Proteomes; UP000054289; Unassembled WGS sequence. DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule. DR CDD; cd02440; AdoMet_MTases; 1. DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1. DR HAMAP; MF_03055; tRNA_methyltr_TrmB_euk; 1. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR InterPro; IPR025763; Trm8_euk. DR InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb. DR PANTHER; PTHR23417; 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE; 1. DR PANTHER; PTHR23417:SF16; TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE; 1. DR Pfam; PF02390; Methyltransf_4; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. DR TIGRFAMs; TIGR00091; tRNA (guanine-N(7)-)-methyltransferase; 1. DR PROSITE; PS51625; SAM_MT_TRMB; 1. PE 3: Inferred from homology; KW Membrane {ECO:0000256|SAM:Phobius}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|HAMAP-Rule:MF_03055}; KW Reference proteome {ECO:0000313|Proteomes:UP000054289}; KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP- KW Rule:MF_03055}; S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_03055}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_03055}; KW Transmembrane {ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|SAM:Phobius}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_03055}; KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP- KW Rule:MF_03055}. FT TRANSMEM 226..243 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT ACT_SITE 197 FT /evidence="ECO:0000256|HAMAP-Rule:MF_03055" FT BINDING 118 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03055" FT BINDING 141..142 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03055" FT BINDING 174..175 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03055" FT BINDING 194 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03055" FT BINDING 272..274 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|HAMAP-Rule:MF_03055" SQ SEQUENCE 293 AA; 35557 MW; 49386052873814B2 CRC64; MKKHKKTPCK RKYRQRAHCN PLSDSYIKYP RNYTYVDWSL HFPLYFEKNQ IKDEYITNKE NSKDEHIVTD NMKNNVENDN MNNVLYLNTN KYPVNYKMRN IFNESYENNK NVTILDIGCG YGGLLFSLSK IFNDKLILGL EIRDKITNYV GEKILSYRYN YHPYYQNISV IRTNVIKFLP NYIKKNQIEK IFFCFPDPHF KRHNWRRRII TIENLSLYYH LLKNNGYIYF ITDVYTLYLW VIFCFSKCPY FKVLTNKDCE NDICVKLIHE SSEESKKVKK NKQNMYYCVA QKI //