ID A0A0L7KB96_PLAFX Unreviewed; 293 AA. AC A0A0L7KB96; DT 11-NOV-2015, integrated into UniProtKB/TrEMBL. DT 11-NOV-2015, sequence version 1. DT 20-JUN-2018, entry version 16. DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; DE EC=2.1.1.33 {ECO:0000256|HAMAP-Rule:MF_03055}; DE AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; DE AltName: Full=tRNA(m7G46)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; GN ORFNames=PFHG_01892 {ECO:0000313|EMBL:KOB60199.1}; OS Plasmodium falciparum (isolate HB3). OC Eukaryota; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; OC Plasmodiidae; Plasmodium; Plasmodium (Laverania). OX NCBI_TaxID=137071 {ECO:0000313|EMBL:KOB60199.1, ECO:0000313|Proteomes:UP000054289}; RN [1] {ECO:0000313|EMBL:KOB60199.1, ECO:0000313|Proteomes:UP000054289} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=HB3 {ECO:0000313|EMBL:KOB60199.1}; RG The Broad Institute Genome Sequencing Platform; RA Volkman S.K., Neafsey D.E., Dash A.P., Chitnis C.E., Hartl D.L., RA Young S.K., Zeng Q., Koehrsen M., Alvarado L., Berlin A., RA Borenstein D., Chapman S.B., Chen Z., Engels R., Freedman E., RA Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E.R., RA Heiman D.I., Howarth C., Jen D., Larson L., Mehta T., Neiman D., RA Park D., Pearson M., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., RA Stolte C., Sykes S., Walk T., White J., Yandava C., Haas B., RA Henn M.R., Nusbaum C., Birren B.; RT "Annotation of Plasmodium falciparum HB3."; RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|Proteomes:UP000054289} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RG The Broad Institute Genome Sequencing Platform; RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Henn M., RA Jaffe D., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., RA Mauceli E., Brockman W., MacCallum I.A., Rounsley S., Young S., RA LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., RA Engels R., Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., RA White J., Kodira C., Zeng Q., Oleary S., Yandava C., Alvarado L., RA Wirth D., Volkman S., Hartl D.; RT "The genome sequence of the Plasmodium falciparum HB3."; RL Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at CC position 46 (m7G46) in tRNA. {ECO:0000256|HAMAP-Rule:MF_03055}. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(46) in tRNA CC = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA. CC {ECO:0000256|HAMAP-Rule:MF_03055, ECO:0000256|SAAS:SAAS00892453}. CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. CC {ECO:0000256|HAMAP-Rule:MF_03055, ECO:0000256|SAAS:SAAS00892463}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|HAMAP-Rule:MF_03055, CC ECO:0000256|SAAS:SAAS00892464}. CC -!- SIMILARITY: Belongs to the class I-like SAM-binding CC methyltransferase superfamily. TrmB family. {ECO:0000256|HAMAP- CC Rule:MF_03055, ECO:0000256|SAAS:SAAS00892467}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CH671956; KOB60199.1; -; Genomic_DNA. DR ProteinModelPortal; A0A0L7KB96; -. DR EnsemblProtists; KOB60199; KOB60199; PFHG_01892. DR UniPathway; UPA00989; -. DR Proteomes; UP000054289; Unassembled WGS sequence. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-UniRule. DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule. DR HAMAP; MF_03055; tRNA_methyltr_TrmB_euk; 1. DR InterPro; IPR029063; SAM-dependent_MTases. DR InterPro; IPR025763; Trm8_euk. DR InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb. DR PANTHER; PTHR23417; PTHR23417; 1. DR PANTHER; PTHR23417:SF16; PTHR23417:SF16; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR TIGRFAMs; TIGR00091; TIGR00091; 1. DR PROSITE; PS51625; SAM_MT_TRMB; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000054289}; KW Membrane {ECO:0000256|SAM:Phobius}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892465}; KW Nucleus {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892446}; KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892449}; KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892461}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892452}; KW Transmembrane {ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|SAM:Phobius}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892456}; KW tRNA-binding {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00892455}. FT TRANSMEM 226 243 Helical. {ECO:0000256|SAM:Phobius}. FT REGION 141 142 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_03055}. FT REGION 174 175 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_03055}. FT REGION 272 274 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_03055}. FT ACT_SITE 197 197 {ECO:0000256|HAMAP-Rule:MF_03055}. FT BINDING 118 118 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|HAMAP-Rule: FT MF_03055}. FT BINDING 194 194 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|HAMAP-Rule: FT MF_03055}. SQ SEQUENCE 293 AA; 35557 MW; 49386052873814B2 CRC64; MKKHKKTPCK RKYRQRAHCN PLSDSYIKYP RNYTYVDWSL HFPLYFEKNQ IKDEYITNKE NSKDEHIVTD NMKNNVENDN MNNVLYLNTN KYPVNYKMRN IFNESYENNK NVTILDIGCG YGGLLFSLSK IFNDKLILGL EIRDKITNYV GEKILSYRYN YHPYYQNISV IRTNVIKFLP NYIKKNQIEK IFFCFPDPHF KRHNWRRRII TIENLSLYYH LLKNNGYIYF ITDVYTLYLW VIFCFSKCPY FKVLTNKDCE NDICVKLIHE SSEESKKVKK NKQNMYYCVA QKI //