ID A0A0H5S397_BRUMA Unreviewed; 347 AA. AC A0A0H5S397; DT 14-OCT-2015, integrated into UniProtKB/TrEMBL. DT 14-OCT-2015, sequence version 1. DT 03-AUG-2022, entry version 35. DE RecName: Full=Calcium uniporter protein {ECO:0000256|RuleBase:RU367035}; GN Name=Bma-mcu-1 {ECO:0000313|EMBL:CRZ22680.1}; GN ORFNames=BM_Bm4745 {ECO:0000313|EMBL:CRZ22680.1}; OS Brugia malayi (Filarial nematode worm). OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; OC Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia. OX NCBI_TaxID=6279 {ECO:0000313|EMBL:CRZ22680.1}; RN [1] {ECO:0000313|EMBL:CRZ22680.1, ECO:0000313|Proteomes:UP000006672} RP NUCLEOTIDE SEQUENCE. RC STRAIN=FR3 {ECO:0000313|EMBL:CRZ22680.1, RC ECO:0000313|Proteomes:UP000006672}; RX PubMed=17885136; DOI=10.1126/science.1145406; RA Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., RA Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., RA Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L., RA Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M., RA Pop M., White O., Barton G.J., Carlow C.K., Crawford M.J., Daub J., RA Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F., RA Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.W., RA Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M., RA McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J., RA Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E., RA Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G., RA Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B., RA Blaxter M.L., Scott A.L.; RT "Draft genome of the filarial nematode parasite Brugia malayi."; RL Science 317:1756-1760(2007). RN [2] {ECO:0000313|EMBL:CRZ22680.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=FR3 {ECO:0000313|EMBL:CRZ22680.1}; RA Gao Y.W., Fan S.T., Sun H.T., Wang Z., Gao X.L., Li Y.G., Wang T.C., RA Zhang K., Xu W.W., Yu Z.J., Xia X.Z.; RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases. RN [3] {ECO:0000313|WBParaSite:Bm4745a.1} RP IDENTIFICATION. RG WormBaseParasite; RL Submitted (DEC-2019) to UniProtKB. CC -!- FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates CC calcium uptake into mitochondria. Constitutes a pore-forming and CC calcium-conducting subunit. Mitochondrial calcium homeostasis plays key CC roles in cellular physiology and regulates cell bioenergetics, CC cytoplasmic calcium signals and activation of cell death pathways. CC {ECO:0000256|RuleBase:RU367035}. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Mitochondrion CC inner membrane {ECO:0000256|ARBA:ARBA00004448, CC ECO:0000256|RuleBase:RU367035}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004448, ECO:0000256|RuleBase:RU367035}. CC -!- DOMAIN: Forms a well-packed pentamer with an overall cylindrical shape. CC The inner core of the pentamer is formed with the second transmembrane CC region and the second coiled-coil region: while the transmembrane CC regions pack into a five-helix bundle having a largely polar pore CC across the membrane, the coiled-coil outside the membrane forms a CC pentamer with a hydrophobic core. The inner core is wrapped by the CC first transmembrane region through contacts between the first and the CC second transmembrane regions. The second transmembrane is followed by CC the inner juxtamembrane region (IJMH) that orients at a wide angle CC relative to the second transmembrane. The two core domains are held CC together on the periphery by the outer juxtamembrane helix (OJMH). CC {ECO:0000256|RuleBase:RU367035}. CC -!- SIMILARITY: Belongs to the MCU (TC 1.A.77) family. CC {ECO:0000256|ARBA:ARBA00005653, ECO:0000256|RuleBase:RU367035}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LN856618; CRZ22680.1; -; Genomic_DNA. DR STRING; 6279.A0A0H5S397; -. DR EnsemblMetazoa; Bm4745a.1; Bm4745a.1; WBGene00225006. DR WBParaSite; Bm4745a.1; Bm4745a.1; WBGene00225006. DR OMA; DDIYVEY; -. DR Proteomes; UP000006672; Unassembled WGS sequence. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005262; F:calcium channel activity; IEA:UniProtKB-UniRule. DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblMetazoa. DR GO; GO:0015292; F:uniporter activity; IEA:UniProtKB-UniRule. DR GO; GO:0036444; P:calcium import into the mitochondrion; IEA:EnsemblMetazoa. DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IEA:UniProtKB-UniRule. DR GO; GO:0051260; P:protein homooligomerization; IEA:EnsemblMetazoa. DR GO; GO:1903426; P:regulation of reactive oxygen species biosynthetic process; IEA:EnsemblMetazoa. DR GO; GO:0061041; P:regulation of wound healing; IEA:EnsemblMetazoa. DR InterPro; IPR006769; MCU_C. DR InterPro; IPR039055; MCU_fam. DR PANTHER; PTHR13462; PTHR13462; 1. DR Pfam; PF04678; MCU; 1. PE 3: Inferred from homology; KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|RuleBase:RU367035}; KW Calcium channel {ECO:0000256|RuleBase:RU367035}; KW Calcium transport {ECO:0000256|RuleBase:RU367035}; KW Ion channel {ECO:0000256|RuleBase:RU367035}; KW Ion transport {ECO:0000256|ARBA:ARBA00023065, KW ECO:0000256|RuleBase:RU367035}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU367035}; KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128, KW ECO:0000256|RuleBase:RU367035}; KW Mitochondrion inner membrane {ECO:0000256|ARBA:ARBA00022792, KW ECO:0000256|RuleBase:RU367035}; KW Reference proteome {ECO:0000313|Proteomes:UP000006672}; KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, KW ECO:0000256|RuleBase:RU367035}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|RuleBase:RU367035}; KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU367035}. FT TRANSMEM 215..234 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU367035" FT TRANSMEM 246..264 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU367035" FT DOMAIN 91..300 FT /note="MCU" FT /evidence="ECO:0000259|Pfam:PF04678" SQ SEQUENCE 347 AA; 40613 MW; 1BE8D3CF29BCA063 CRC64; MRCRWYSITG LLQQRQQHLQ IQLSTCYINL NNMHHLRRCS NDTTRGNLHI WCQSGLPLLE VPLPSRREIC QFTLKPISDT VSRFCKNIEC EDKGIEIVYV YSTSGNRIAG STLLKHLLLR GNFRLRINDF IYTVEVPSSK DVIANIMADG DVMQKFDGLR STIAPLYSIM NVEVYKAFQE RLLIEEYENV KAELKPLIQA KFKFDEFCRK RAQRILWLTL AAMGFQAGFL ARLTWWDYSW DVMEPITYFA THATLIASFA YYLYTNQYYN HNDHKKRAIS LYFHKKAAKS NFDISRFNEL QNVASSIKHD LKKLRDPLYQ HLPTARLASL LQESGKVQPR SFTKERS //