ID A0A0G4IHI0_PLABS Unreviewed; 260 AA. AC A0A0G4IHI0; DT 16-SEP-2015, integrated into UniProtKB/TrEMBL. DT 16-SEP-2015, sequence version 1. DT 17-FEB-2016, entry version 5. DE RecName: Full=tRNA (guanine-N(7)-)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; DE EC=2.1.1.33 {ECO:0000256|HAMAP-Rule:MF_03055}; DE AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; DE AltName: Full=tRNA(m7G46)-methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055}; GN ORFNames=PBRA_000466 {ECO:0000313|EMBL:CEO94681.1}; OS Plasmodiophora brassicae (Clubroot disease). OC Eukaryota; Rhizaria; Cercozoa; Plasmodiophorida; Plasmodiophoridae; OC Plasmodiophora. OX NCBI_TaxID=37360 {ECO:0000313|EMBL:CEO94681.1, ECO:0000313|Proteomes:UP000039324}; RN [1] {ECO:0000313|EMBL:CEO94681.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=E3 {ECO:0000313|EMBL:CEO94681.1}; RA Chooi Y.-H.; RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at CC position 46 (m7G46) in tRNA. {ECO:0000256|HAMAP-Rule:MF_03055}. CC -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + guanine(46) in tRNA CC = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA. CC {ECO:0000256|HAMAP-Rule:MF_03055, ECO:0000256|SAAS:SAAS00496400}. CC -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis. CC {ECO:0000256|HAMAP-Rule:MF_03055, ECO:0000256|SAAS:SAAS00496399}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|HAMAP-Rule:MF_03055, CC ECO:0000256|SAAS:SAAS00496410}. CC -!- SIMILARITY: Belongs to the class I-like SAM-binding CC methyltransferase superfamily. TrmB family. {ECO:0000256|HAMAP- CC Rule:MF_03055}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CDSF01000001; CEO94681.1; -; Genomic_DNA. DR EnsemblProtists; CEO94681; CEO94681; PBRA_000466. DR UniPathway; UPA00989; -. DR Proteomes; UP000039324; Unassembled WGS sequence. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.50.150; -; 1. DR HAMAP; MF_03055; tRNA_methyltr_TrmB_euk; 1. DR InterPro; IPR029063; SAM-dependent_MTases. DR InterPro; IPR025763; Trm8_euk. DR InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase_Trmb. DR Pfam; PF02390; Methyltransf_4; 1. DR SUPFAM; SSF53335; SSF53335; 1. DR TIGRFAMs; TIGR00091; TIGR00091; 1. DR PROSITE; PS51625; SAM_MT_TRMB; 1. PE 3: Inferred from homology; KW Complete proteome {ECO:0000313|Proteomes:UP000039324}; KW Methyltransferase {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496395}; KW Nucleus {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496406}; KW Reference proteome {ECO:0000313|Proteomes:UP000039324}; KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496396}; KW S-adenosyl-L-methionine {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496409}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496403}; KW tRNA processing {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496397}; KW tRNA-binding {ECO:0000256|HAMAP-Rule:MF_03055, KW ECO:0000256|SAAS:SAAS00496398}. FT REGION 97 98 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_03055}. FT REGION 140 141 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_03055}. FT REGION 237 239 S-adenosyl-L-methionine binding. FT {ECO:0000256|HAMAP-Rule:MF_03055}. FT ACT_SITE 163 163 {ECO:0000256|HAMAP-Rule:MF_03055}. FT BINDING 74 74 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|HAMAP-Rule: FT MF_03055}. FT BINDING 160 160 S-adenosyl-L-methionine; via carbonyl FT oxygen. {ECO:0000256|HAMAP-Rule: FT MF_03055}. SQ SEQUENCE 260 AA; 29679 MW; 41D4672C674D4518 CRC64; MPRQFQMSLP FQRVQGKRFV RIRAHVNPLS FSDQYVCPDG PSSVVWKEYF PKFPGVSDDA PAGLDDTVRF VDAGCGFGGL LEALAPTFPN KMILGMEIRE QVVKHVNTRL NCLQAGSKGA SEWKSEDIPA YSNIGVCHTN VMKYMVKFFQ KGQLEKLFFL FPDPHFKRSN HRRRIISPNL LSEYAYVLQV GGLIYNVTDV EDLFNWTVTH FEAHPLFERL SEVEVAADPA VPAMFQTDEA RRVKKNNGTT FVSVWRRIQD //