ID A0A0G2JGL0_MOUSE Unreviewed; 148 AA. AC A0A0G2JGL0; DT 22-JUL-2015, integrated into UniProtKB/TrEMBL. DT 22-JUL-2015, sequence version 1. DT 29-MAY-2024, entry version 46. DE RecName: Full=Ubiquitin-conjugating enzyme E2 D3 {ECO:0000256|ARBA:ARBA00040338}; DE EC=2.3.2.23 {ECO:0000256|ARBA:ARBA00012486}; DE EC=2.3.2.24 {ECO:0000256|ARBA:ARBA00039076}; DE AltName: Full=(E3-independent) E2 ubiquitin-conjugating enzyme D3 {ECO:0000256|ARBA:ARBA00041314}; DE AltName: Full=E2 ubiquitin-conjugating enzyme D3 {ECO:0000256|ARBA:ARBA00041427}; DE AltName: Full=Ubiquitin carrier protein D3 {ECO:0000256|ARBA:ARBA00041862}; DE AltName: Full=Ubiquitin-protein ligase D3 {ECO:0000256|ARBA:ARBA00043109}; GN Name=Ube2d3 {ECO:0000313|Ensembl:ENSMUSP00000143606.2, GN ECO:0000313|MGI:MGI:1913355}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000143606.2, ECO:0000313|Proteomes:UP000000589}; RN [1] {ECO:0000313|Ensembl:ENSMUSP00000143606.2, ECO:0000313|Proteomes:UP000000589} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000143606.2, RC ECO:0000313|Proteomes:UP000000589}; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [2] {ECO:0000313|Ensembl:ENSMUSP00000143606.2} RP IDENTIFICATION. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000143606.2}; RG Ensembl; RL Submitted (FEB-2024) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin- CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin- CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23; CC Evidence={ECO:0000256|ARBA:ARBA00000485}; CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + CC [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L- CC cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.; CC EC=2.3.2.24; Evidence={ECO:0000256|ARBA:ARBA00035845}; CC -!- PATHWAY: Protein modification. {ECO:0000256|ARBA:ARBA00043952}. CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. CC {ECO:0000256|RuleBase:RU362109}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR AlphaFoldDB; A0A0G2JGL0; -. DR SMR; A0A0G2JGL0; -. DR jPOST; A0A0G2JGL0; -. DR ProteomicsDB; 357873; -. DR Ensembl; ENSMUST00000197134.3; ENSMUSP00000143606.2; ENSMUSG00000078578.10. DR AGR; MGI:1913355; -. DR MGI; MGI:1913355; Ube2d3. DR VEuPathDB; HostDB:ENSMUSG00000078578; -. DR GeneTree; ENSGT00940000153169; -. DR OMA; RNCKEIL; -. DR OrthoDB; 5478564at2759; -. DR ChiTaRS; Ube2d3; mouse. DR Proteomes; UP000000589; Chromosome 3. DR Bgee; ENSMUSG00000078578; Expressed in undifferentiated genital tubercle and 260 other cell types or tissues. DR ExpressionAtlas; A0A0G2JGL0; baseline and differential. DR GO; GO:0005634; C:nucleus; IEA:TreeGrafter. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; IEA:TreeGrafter. DR GO; GO:0070979; P:protein K11-linked ubiquitination; IEA:TreeGrafter. DR GO; GO:0070936; P:protein K48-linked ubiquitination; IEA:TreeGrafter. DR CDD; cd00195; UBCc; 1. DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1. DR InterPro; IPR000608; UBQ-conjugat_E2. DR InterPro; IPR023313; UBQ-conjugating_AS. DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD. DR PANTHER; PTHR24068; UBIQUITIN-CONJUGATING ENZYME E2; 1. DR PANTHER; PTHR24068:SF415; UBIQUITIN-CONJUGATING ENZYME E2 D3; 1. DR Pfam; PF00179; UQ_con; 1. DR SMART; SM00212; UBCc; 1. DR SUPFAM; SSF54495; UBC-like; 1. DR PROSITE; PS00183; UBC_1; 1. DR PROSITE; PS50127; UBC_2; 1. PE 1: Evidence at protein level; KW ATP-binding {ECO:0000256|RuleBase:RU362109}; KW Nucleotide-binding {ECO:0000256|RuleBase:RU362109}; KW Proteomics identification {ECO:0007829|EPD:A0A0G2JGL0, KW ECO:0007829|MaxQB:A0A0G2JGL0}; KW Reference proteome {ECO:0000313|Proteomes:UP000000589}; KW Transferase {ECO:0000256|ARBA:ARBA00022679}; KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786, KW ECO:0000256|RuleBase:RU362109}. FT DOMAIN 1..147 FT /note="UBC core" FT /evidence="ECO:0000259|PROSITE:PS50127" FT ACT_SITE 85 FT /note="Glycyl thioester intermediate" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10133" SQ SEQUENCE 148 AA; 16785 MW; E73DC194DB6D4EFE CRC64; MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD RDKYNRLARE WTEKYAML //