ID   A0A0F2KM72_9PROT        Unreviewed;       288 AA.
AC   A0A0F2KM72;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   07-APR-2021, entry version 25.
DE   SubName: Full=3-hydroxybutyryl-CoA dehydrogenase {ECO:0000313|EMBL:KJR62347.1};
GN   ORFNames=VY88_25695 {ECO:0000313|EMBL:KJR62347.1};
OS   Azospirillum thiophilum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales;
OC   Rhodospirillaceae; Azospirillum.
OX   NCBI_TaxID=528244 {ECO:0000313|EMBL:KJR62347.1, ECO:0000313|Proteomes:UP000033623};
RN   [1] {ECO:0000313|EMBL:KJR62347.1, ECO:0000313|Proteomes:UP000033623}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 21654 {ECO:0000313|EMBL:KJR62347.1,
RC   ECO:0000313|Proteomes:UP000033623};
RA   Kwak Y., Shin J.-H.;
RT   "Genome sequence of Azospirillum thiophilum strain DSM 21654T.";
RL   Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KJR62347.1}.
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DR   EMBL; LAEL01000005; KJR62347.1; -; Genomic_DNA.
DR   RefSeq; WP_045584909.1; NZ_LAEL01000005.1.
DR   EnsemblBacteria; KJR62347; KJR62347; VY88_25695.
DR   KEGG; ati:AL072_28615; -.
DR   PATRIC; fig|528244.3.peg.5350; -.
DR   OrthoDB; 862072at2; -.
DR   Proteomes; UP000033623; Unassembled WGS sequence.
DR   GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0006631; P:fatty acid metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.1040.10; -; 1.
DR   InterPro; IPR022694; 3-OHacyl-CoA_DH.
DR   InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR   InterPro; IPR006108; 3HC_DH_C.
DR   InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR   InterPro; IPR013328; 6PGD_dom2.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00725; 3HCDH; 1.
DR   Pfam; PF02737; 3HCDH_N; 1.
DR   PIRSF; PIRSF000105; HCDH; 1.
DR   SUPFAM; SSF48179; SSF48179; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   4: Predicted;
KW   NAD {ECO:0000256|PIRSR:PIRSR000105-2}.
FT   DOMAIN          9..187
FT                   /note="3HCDH_N"
FT                   /evidence="ECO:0000259|Pfam:PF02737"
FT   DOMAIN          190..286
FT                   /note="3HCDH"
FT                   /evidence="ECO:0000259|Pfam:PF00725"
FT   NP_BIND         14..19
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   BINDING         37
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   BINDING         53
FT                   /note="Coenzyme A"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-3"
FT   BINDING         60
FT                   /note="Coenzyme A"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-3"
FT   BINDING         96
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   BINDING         101
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   BINDING         123
FT                   /note="Coenzyme A"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-3"
FT   BINDING         123
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   BINDING         147
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   BINDING         278
FT                   /note="NAD"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-2"
FT   SITE            144
FT                   /note="Important for catalytic activity"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000105-1"
SQ   SEQUENCE   288 AA;  30474 MW;  89B6546C98951A03 CRC64;
     MAESKGIETV GVIGAGQMGN GIAQVCAVAG LTVVMTDISD AAVQRGLSTV GGSLERLVKK
     EKMSAADREA ALARITATTD KAALAACDLV IEAATENEDL KVKILKDLCA ILKPEALVAT
     NTSSISITKL ATATDRPDRF IGLHFFNPVP LMALLEIIRG IQTSDDTHAK AMAFAKRVGK
     SPITAKNSPG FAVNRILCPM INEAIFALQE GIATAEDIDA GMKAGCNHPI GPLALADLIG
     LDTMLSVMEV FYEGFNDPKY RPAPLLKEMV DAGYLGRKTG RGFYDYSK
//