ID A0A0E2P7E7_9STRE Unreviewed; 451 AA. AC A0A0E2P7E7; DT 27-MAY-2015, integrated into UniProtKB/TrEMBL. DT 27-MAY-2015, sequence version 1. DT 22-FEB-2023, entry version 26. DE SubName: Full=RNA methyltransferase {ECO:0000313|EMBL:ETE06551.1}; GN ORFNames=U751_03635 {ECO:0000313|EMBL:ETE06551.1}; OS Streptococcus pseudopneumoniae 22725. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=1415759 {ECO:0000313|EMBL:ETE06551.1, ECO:0000313|Proteomes:UP000018701}; RN [1] {ECO:0000313|EMBL:ETE06551.1, ECO:0000313|Proteomes:UP000018701} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=22725 {ECO:0000313|EMBL:ETE06551.1, RC ECO:0000313|Proteomes:UP000018701}; RA Ikryannikova L.N., Lominadze G.G., Ilina E.N., Kostryukova E.S., RA Karpova I.Y., Semashko T.A., Larin A.K., Ischenko D.S., Mayansky N.A., RA Govorun V.M.; RT "Genome sequencing of Streptococcus pseudopneumoniae strains."; RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases. CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE- CC ProRule:PRU01024}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:ETE06551.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AYRO01000005; ETE06551.1; -; Genomic_DNA. DR RefSeq; WP_000914557.1; NZ_AYRO01000005.1. DR AlphaFoldDB; A0A0E2P7E7; -. DR EnsemblBacteria; ETE06551; ETE06551; U751_03635. DR PATRIC; fig|1415759.3.peg.737; -. DR Proteomes; UP000018701; Unassembled WGS sequence. DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro. DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt. DR CDD; cd02440; AdoMet_MTases; 1. DR Gene3D; 2.40.50.1070; -; 1. DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1. DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1. DR InterPro; IPR030390; MeTrfase_TrmA_AS. DR InterPro; IPR030391; MeTrfase_TrmA_CS. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR InterPro; IPR002792; TRAM_dom. DR InterPro; IPR010280; U5_MeTrfase_fam. DR PANTHER; PTHR11061:SF48; 23S RRNA (URACIL(747)-C(5))-METHYLTRANSFERASE RLMC; 1. DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1. DR Pfam; PF05958; tRNA_U5-meth_tr; 1. DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1. DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1. DR TIGRFAMs; TIGR00479; rumA; 1. DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1. DR PROSITE; PS50926; TRAM; 1. DR PROSITE; PS01230; TRMA_1; 1. DR PROSITE; PS01231; TRMA_2; 1. PE 3: Inferred from homology; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE- KW ProRule:PRU01024}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, KW ECO:0000256|PROSITE-ProRule:PRU01024}; KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE- KW ProRule:PRU01024}. FT DOMAIN 1..59 FT /note="TRAM" FT /evidence="ECO:0000259|PROSITE:PS50926" FT ACT_SITE 408 FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015" FT ACT_SITE 408 FT /note="Nucleophile" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 283 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 312 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 333 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" FT BINDING 381 FT /ligand="S-adenosyl-L-methionine" FT /ligand_id="ChEBI:CHEBI:59789" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024" SQ SEQUENCE 451 AA; 50985 MW; 891E0FD3D91199C1 CRC64; MLKKNDIVEV EIVDLTHEGA GVAKVDGLVF FVENALPSEK ILMRVLKVNK KIGFGKVEEY LVQSPHRNQD LDLAYLRSGI ADLGHLAYPE QLKFKTKQVK DSLYKIAGID DIEVAETLGM ENPVKYRNKA QVPVRRVNGV LETGFFRKNS HDLMPLEDFF IQDSVIDEVV VGLRDLLRRY DLKPYDEKEQ SGLIRNLVVR RGHYSGQIMV ILVTTRPKVF RVDQLIEQVI KQFPEIVSVM QNINDQNTNA IFGKEWRTLY GQDFITDQML GNDYQIAGPA FYQVNTEMAE KLYQTAIDFA ELKEDDVVID AYSGIGTIGI SVAKHVKEVY GVEVIPEAVE NSKKNAQLNN ISNANYVCDT AENAMKNWLK DGIQPSLILV DPPRKGLTES FIKASTQTGA NRIAYISCNV ATMARDIKLY QELGYELKKV QPVDLFPQTH HVECVALLVK T //