ID A0A0D8BWP6_GEOKU Unreviewed; 302 AA. AC A0A0D8BWP6; DT 27-MAY-2015, integrated into UniProtKB/TrEMBL. DT 27-MAY-2015, sequence version 1. DT 20-JAN-2016, entry version 6. DE RecName: Full=Homoserine O-succinyltransferase {ECO:0000256|HAMAP-Rule:MF_00295, ECO:0000256|SAAS:SAAS00382189}; DE EC=2.3.1.46 {ECO:0000256|HAMAP-Rule:MF_00295, ECO:0000256|SAAS:SAAS00382204}; DE AltName: Full=Homoserine O-transsuccinylase {ECO:0000256|HAMAP-Rule:MF_00295}; GN Name=metA {ECO:0000256|HAMAP-Rule:MF_00295, GN ECO:0000313|EMBL:KJE28404.1}; GN ORFNames=LG52_1351 {ECO:0000313|EMBL:KJE28404.1}; OS Geobacillus kaustophilus. OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus. OX NCBI_TaxID=1462 {ECO:0000313|EMBL:KJE28404.1, ECO:0000313|Proteomes:UP000032522}; RN [1] {ECO:0000313|EMBL:KJE28404.1, ECO:0000313|Proteomes:UP000032522} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Et7/4 {ECO:0000313|EMBL:KJE28404.1, RC ECO:0000313|Proteomes:UP000032522}; RA Filippidou S., Jeanneret N., Russel-Delif L., Junier T., Wunderlin T., RA Molina V., Johnson S.L., Davenport K.W., Chain P.S., Dorador C., RA Junier P.; RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:CTQ31226.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=DSM 7263 {ECO:0000313|EMBL:CTQ31226.1}; RA De Berardinis V.; RT "Methionin biosynthesis."; RL Submitted (JUN-2015) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: Succinyl-CoA + L-homoserine = CoA + O- CC succinyl-L-homoserine. {ECO:0000256|HAMAP-Rule:MF_00295, CC ECO:0000256|SAAS:SAAS00382172}. CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de CC novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1. CC {ECO:0000256|HAMAP-Rule:MF_00295, ECO:0000256|SAAS:SAAS00382153}. CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00295, CC ECO:0000256|SAAS:SAAS00382156}. CC -!- SIMILARITY: Belongs to the HTS family. {ECO:0000256|HAMAP- CC Rule:MF_00295}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; LN871223; CTQ31226.1; -; Genomic_DNA. DR EMBL; JYBP01000003; KJE28404.1; -; Genomic_DNA. DR RefSeq; WP_014195890.1; NZ_JYBP01000003.1. DR EnsemblBacteria; KJE28404; KJE28404; LG52_1351. DR UniPathway; UPA00051; UER00075. DR Proteomes; UP000032522; Unassembled WGS sequence. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0008899; F:homoserine O-succinyltransferase activity; IEA:UniProtKB-HAMAP. DR GO; GO:0019281; P:L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine; IEA:UniProtKB-HAMAP. DR Gene3D; 3.40.50.880; -; 1. DR HAMAP; MF_00295; Homoser_O_succinyltr; 1. DR InterPro; IPR029062; Class_I_gatase-like. DR InterPro; IPR005697; Homserine_suc_trans. DR PANTHER; PTHR20919; PTHR20919; 1. DR Pfam; PF04204; HTS; 1. DR PIRSF; PIRSF000450; H_ser_succinyltr; 1. DR ProDom; PD037892; Homserine_suc_trans; 1. DR SUPFAM; SSF52317; SSF52317; 1. DR TIGRFAMs; TIGR01001; metA; 1. PE 3: Inferred from homology; KW Acyltransferase {ECO:0000256|HAMAP-Rule:MF_00295, KW ECO:0000256|SAAS:SAAS00084645, ECO:0000313|EMBL:KJE28404.1}; KW Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00295, KW ECO:0000256|SAAS:SAAS00084546}; KW Complete proteome {ECO:0000313|Proteomes:UP000032522}; KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00295, KW ECO:0000256|SAAS:SAAS00084567}; KW Methionine biosynthesis {ECO:0000256|HAMAP-Rule:MF_00295, KW ECO:0000256|SAAS:SAAS00084638}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_00295, KW ECO:0000256|SAAS:SAAS00084624, ECO:0000313|EMBL:KJE28404.1}. FT ACT_SITE 142 142 {ECO:0000256|HAMAP-Rule:MF_00295, FT ECO:0000256|PIRSR:PIRSR000450-1}. SQ SEQUENCE 302 AA; 35281 MW; E67E673AC17F5968 CRC64; MPINIPKDLP AKEILEQENI FVMDEERAYS QDIRPLNIII LNLMPEKEKA ETQLLRLLGN SPLQVNVTFL RPATHEPKTT SKHHLEQFYT IFPHIRHRKF DGMIITGAPV EQLPFEEVTY WDELTDIMEW TKTNVTSTLH ICWGAQAGLY YHYGIPKYPL PEKCFGVFNH TVEAKNVKLL RGFDDVFRMP HSRHTDVKRE DIEKVPDLTI LSMSDKAGVC LVASNDGRRI FLTGHPEYDA TTLKEEYERD LAKGLPIHIP ESYFPNDDPS QPPLNTWRSH ANLLFVNWLN YYVYQETPYE WE //