ID A0A0D2XB04_FUSO4 Unreviewed; 779 AA. AC A0A0D2XB04; DT 29-APR-2015, integrated into UniProtKB/TrEMBL. DT 29-APR-2015, sequence version 1. DT 07-APR-2021, entry version 35. DE RecName: Full=Urea amidohydrolase {ECO:0000256|ARBA:ARBA00020201}; DE EC=3.5.1.5 {ECO:0000256|ARBA:ARBA00012934}; DE AltName: Full=Urease {ECO:0000256|ARBA:ARBA00013883}; GN ORFNames=FOXG_01071 {ECO:0000313|EMBL:KNA95550.1}; OS Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 OS / NRRL 34936) (Fusarium vascular wilt of tomato). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; OC Fusarium oxysporum species complex. OX NCBI_TaxID=426428 {ECO:0000313|EnsemblFungi:FOXG_01071P0}; RN [1] {ECO:0000313|EMBL:KNA95550.1} RP NUCLEOTIDE SEQUENCE. RC STRAIN=4287 {ECO:0000313|EMBL:KNA95550.1}; RG The Broad Institute Genome Sequencing Platform; RA Birren B., Lander E., Galagan J., Nusbaum C., Devon K., Ma L.-J., Jaffe D., RA Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M., Mauceli E., RA Brockman W., MacCallum I.A., Young S., LaButti K., DeCaprio D., RA Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., RA Larson L., White J., O'Leary S., Kodira C., Zeng Q., Yandava C., RA Alvarado L., Kistler C., Shim W.-B., Kang S., Woloshuk C.; RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|EMBL:KNA95550.1, ECO:0000313|EnsemblFungi:FOXG_01071P0} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=4287 {ECO:0000313|EMBL:KNA95550.1}, and 4287 / CBS 123668 / RC FGSC 9935 / NRRL 34936 {ECO:0000313|EnsemblFungi:FOXG_01071P0}; RX PubMed=20237561; DOI=10.1038/nature08850; RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J., RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B., RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R., RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W., RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J., RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E., RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M., RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D., RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A., RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C., RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S., RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.; RT "Comparative genomics reveals mobile pathogenicity chromosomes in RT Fusarium."; RL Nature 464:367-373(2010). RN [3] {ECO:0000313|EnsemblFungi:FOXG_01071P0} RP IDENTIFICATION. RC STRAIN=4287 / CBS 123668 / FGSC 9935 / NRRL 34936 RC {ECO:0000313|EnsemblFungi:FOXG_01071P0}; RG EnsemblFungi; RL Submitted (MAR-2015) to UniProtKB. CC -!- CATALYTIC ACTIVITY: CC Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199, CC ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5; CC Evidence={ECO:0000256|ARBA:ARBA00000242}; CC -!- COFACTOR: CC Name=Ni cation; Xref=ChEBI:CHEBI:25516; CC Evidence={ECO:0000256|PIRSR:PIRSR611612-51}; CC Note=Binds 2 nickel ions per subunit. {ECO:0000256|PIRSR:PIRSR611612- CC 51}; CC -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from CC urea (urease route): step 1/1. {ECO:0000256|ARBA:ARBA00004897}. CC -!- PTM: Carbamylation allows a single lysine to coordinate two nickel CC ions. {ECO:0000256|PIRSR:PIRSR611612-50}. CC -!- SIMILARITY: In the C-terminal section; belongs to the metallo-dependent CC hydrolases superfamily. Urease alpha subunit family. CC {ECO:0000256|ARBA:ARBA00007966}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DS231696; KNA95550.1; -; Genomic_DNA. DR EMBL; DS231696; KNA95551.1; -; Genomic_DNA. DR EMBL; DS231696; KNA95552.1; -; Genomic_DNA. DR RefSeq; XP_018233596.1; XM_018377804.1. DR RefSeq; XP_018233597.1; XM_018377805.1. DR RefSeq; XP_018233598.1; XM_018377806.1. DR MEROPS; M38.982; -. DR EnsemblFungi; FOXG_01071T0; FOXG_01071P0; FOXG_01071. DR GeneID; 28943345; -. DR OMA; GFDSHIH; -. DR UniPathway; UPA00258; UER00370. DR Proteomes; UP000009097; Chromosome 1. DR GO; GO:0035550; C:urease complex; IEA:InterPro. DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro. DR GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC. DR GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway. DR CDD; cd00375; Urease_alpha; 1. DR CDD; cd00407; Urease_beta; 1. DR Gene3D; 2.10.150.10; -; 1. DR Gene3D; 2.30.40.10; -; 1. DR Gene3D; 3.30.280.10; -; 1. DR HAMAP; MF_01953; Urease_alpha; 1. DR HAMAP; MF_01954; Urease_beta; 1. DR InterPro; IPR006680; Amidohydro-rel. DR InterPro; IPR011059; Metal-dep_hydrolase_composite. DR InterPro; IPR032466; Metal_Hydrolase. DR InterPro; IPR011612; Urease_alpha_N_dom. DR InterPro; IPR017950; Urease_AS. DR InterPro; IPR005848; Urease_asu. DR InterPro; IPR017951; Urease_asu_c. DR InterPro; IPR002019; Urease_beta. DR InterPro; IPR036461; Urease_betasu_sf. DR InterPro; IPR002026; Urease_gamma/gamma-beta_su. DR InterPro; IPR036463; Urease_gamma_sf. DR InterPro; IPR029754; Urease_Ni-bd. DR Pfam; PF01979; Amidohydro_1; 1. DR Pfam; PF00449; Urease_alpha; 1. DR Pfam; PF00699; Urease_beta; 1. DR Pfam; PF00547; Urease_gamma; 1. DR PRINTS; PR01752; UREASE. DR SUPFAM; SSF51278; SSF51278; 1. DR SUPFAM; SSF51338; SSF51338; 1. DR SUPFAM; SSF51556; SSF51556; 1. DR SUPFAM; SSF54111; SSF54111; 1. DR TIGRFAMs; TIGR01792; urease_alph; 1. DR TIGRFAMs; TIGR00192; urease_beta; 1. DR PROSITE; PS01120; UREASE_1; 1. DR PROSITE; PS00145; UREASE_2; 1. DR PROSITE; PS51368; UREASE_3; 1. PE 3: Inferred from homology; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE- KW ProRule:PRU00700}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, KW ECO:0000256|PIRSR:PIRSR611612-51}; KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|PIRSR:PIRSR611612-51}; KW Reference proteome {ECO:0000313|Proteomes:UP000009097}. FT DOMAIN 343..779 FT /note="Urease" FT /evidence="ECO:0000259|PROSITE:PS51368" FT ACT_SITE 534 FT /note="Proton donor" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-52, FT ECO:0000256|PROSITE-ProRule:PRU00700" FT METAL 348 FT /note="Nickel 1; via tele nitrogen" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT METAL 350 FT /note="Nickel 1; via tele nitrogen" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT METAL 431 FT /note="Nickel 1; via carbamate group" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT METAL 431 FT /note="Nickel 2; via carbamate group" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT METAL 460 FT /note="Nickel 2; via pros nitrogen" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT METAL 486 FT /note="Nickel 2; via tele nitrogen" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT METAL 574 FT /note="Nickel 1" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-51" FT BINDING 433 FT /note="Substrate" FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00700" FT MOD_RES 431 FT /note="N6-carboxylysine" FT /evidence="ECO:0000256|PIRSR:PIRSR611612-50" SQ SEQUENCE 779 AA; 83745 MW; C0930F6874728716 CRC64; MALGATMLGR RHVLPSVCTT LHEIQVEGTF PSGTYLVTVH NPISSDDGDL RRALYGSFLP VPDNSIFPMA ATEDYQLDKQ PGAVVPVKTK KITLNEGRKR IRLQVTSTGD RPIQVGSHYH FIETNPQLEF DRIRAYGYRL DIPAGTSVRF EPGDTKTVTL VEIGGNRVIR GGNNLASGVV DLSRADEILA RLQEAGYAHK PDPAGDMAHI DVFQMDHASY ATMFGPTTGD LVRLGSTDLW IKVERDETVY GDECKFGGGK TLREGMGQAT GRHDADTLDL VVTNALIVDW TGIYKADIGV KEGMIVGIGK AGNPDVMDGV TEGMVVGSCT DVVAGEGKIV TAGAIDTHIH FICPQQVPEA LASGVTTMLG GGTGPSAGTN ATTCTPGAHY MRQMLQACDQ LPINIGITGK GNDSSPEGLR DQVNAGACGL KLHEDWGCTP AAIDACLSVC DEFDIQCLIH TDTLNESGFV ESTIAAFKNR TIHTYHTEGA GGGHAPDIIS VVEHQNVLPS STNPTRPFTR NTLDEHLDML MVCHHLSKNI PEDVAFAESR IRAETIAAED VLHDKGAISM MSSDSQAMGR CGEVVLRTWN TAHKNKVQRG WLPEDEGTGA DNARVKRYVS KYTINPAIAQ GFGHVIGSIE VGKFADLVLW DPAWFGTKPS HVLKGGHIAY AQMGDPNASI PTVQPIIARP MFSPHCASTS ILFVSSASIE TGAIASYGLR SRVEAVKGCR NIGKSDMRHN DLKPKMRVDP ESYTVEADGE VCVAAPAETL PLTQQFYVY //