ID CSM5_STRTR Reviewed; 357 AA. AC A0A0A7HF79; DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot. DT 04-MAR-2015, sequence version 1. DT 22-FEB-2023, entry version 22. DE RecName: Full=CRISPR system Cms protein Csm5; DE AltName: Full=CRISPR type III A-associated protein Csm5; GN Name=csm5 {ECO:0000303|PubMed:25458845}; OS Streptococcus thermophilus. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=1308; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PHAGE RESISTANCE, TARGETS RP SSRNA, SUBUNIT, AND ANTIVIRAL DEFENSE. RC STRAIN=DGCC8004; RX PubMed=25458845; DOI=10.1016/j.molcel.2014.09.027; RA Tamulaitis G., Kazlauskiene M., Manakova E., Venclovas C., Nwokeoji A.O., RA Dickman M.J., Horvath P., Siksnys V.; RT "Programmable RNA shredding by the type III-A CRISPR-Cas system of RT Streptococcus thermophilus."; RL Mol. Cell 56:506-517(2014). RN [2] RP SUBUNIT. RC STRAIN=DGCC8004; RX PubMed=27105119; DOI=10.1016/j.molcel.2016.03.024; RA Kazlauskiene M., Tamulaitis G., Kostiuk G., Venclovas C., Siksnys V.; RT "Spatiotemporal control of type III-A CRISPR-Cas immunity: coupling DNA RT degradation with the target RNA recognition."; RL Mol. Cell 62:295-306(2016). RN [3] RP SUBUNIT. RC STRAIN=DGCC8004; RX PubMed=28663439; DOI=10.1126/science.aao0100; RA Kazlauskiene M., Kostiuk G., Venclovas C., Tamulaitis G., Siksnys V.; RT "A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas RT systems."; RL Science 357:605-609(2017). CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic CC repeat) is an adaptive immune system that provides protection against CC mobile genetic elements (viruses, transposable elements and conjugative CC plasmids). CRISPR clusters contain spacers, sequences complementary to CC antecedent mobile elements, and target invading nucleic acids. CRISPR CC clusters are transcribed and processed into CRISPR RNA (crRNA). The CC type III-A Csm effector complex binds crRNA and acts as a crRNA-guided CC RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA CC cognate to the crRNA is required for all activities. In a heterologous CC host this Csm effector complex restricts ssRNA phage MS2, suggesting it CC may target RNA viruses in vivo. {ECO:0000269|PubMed:25458845}. CC -!- FUNCTION: Csm functions as a non-specific ssDNase. Base-pairing between CC crRNA and target RNA to form a ternary Csm complex activates a ssDNase CC activity; target RNA cleavage suppresses the ssDNase, a temporal CC control that prevents uncontrolled DNA degradation. Viral RNA CC transcripts probably tether the Csm complex to the viral genome, CC recruiting Cas10 ssDNA activity which is able to degrade DNA in the CC transcription bubble, spatially controlling the DNase activity. CC {ECO:0000269|PubMed:27105119}. CC -!- FUNCTION: This subunit might be involved in maturation of a crRNA CC intermediate to its mature form. {ECO:0000305|PubMed:25458845}. CC -!- SUBUNIT: Part of the Csm effector complex that includes at least CC Cas10(1), Csm2(3), Csm3(5), Csm4(1), Csm5(1) and mature crRNA CC (PubMed:25458845, PubMed:27105119, PubMed:28663439). The Csm complex is CC elongated and slightly twisted with a maximal length of 215 Angstroms CC and a diameter of 75-80 Angstroms (PubMed:25458845). It has been CC modeled to have a central protein filamant of Csm3 subunits along which CC the dsRNA helix of paired crRNA and target RNA binds. The filament is CC capped at one end by Cas10 and Csm4 and at the other end by Csm5; ssDNA CC is thought to bind to the N-terminal HD domain of Cas10 (Probable). Csm CC with a precursor crRNA does not include Csm5, while Cas6, the enzyme CC probably involved in pre-crRNA processing, is found associated with a CC subset of the Csm complex (PubMed:25458845). CC {ECO:0000269|PubMed:25458845, ECO:0000269|PubMed:27105119, CC ECO:0000269|PubMed:28663439, ECO:0000305|PubMed:25458845, CC ECO:0000305|PubMed:27105119}. CC -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus. CC {ECO:0000269|PubMed:25458845}. CC -!- SIMILARITY: Belongs to the CRISPR-associated Csm5 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KM222358; AIZ03608.1; -; Genomic_DNA. DR RefSeq; WP_014621550.1; NZ_CP049053.1. DR AlphaFoldDB; A0A0A7HF79; -. DR SMR; A0A0A7HF79; -. DR STRING; 322159.STER_0977; -. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW. DR InterPro; IPR010173; CRISPR-assoc_Csm5. DR InterPro; IPR005537; RAMP_III_fam. DR PANTHER; PTHR38007; CRISPR SYSTEM CMS PROTEIN CSM5; 1. DR PANTHER; PTHR38007:SF1; CRISPR SYSTEM CMS PROTEIN CSM5; 1. DR Pfam; PF03787; RAMPs; 1. DR TIGRFAMs; TIGR01899; cas_TM1807_csm5; 1. PE 1: Evidence at protein level; KW Antiviral defense; RNA-binding. FT CHAIN 1..357 FT /note="CRISPR system Cms protein Csm5" FT /id="PRO_0000446124" SQ SEQUENCE 357 AA; 41161 MW; 51C66D957D73F157 CRC64; MKNDYRTFKL SLLTLAPIHI GNGEKYTSRE FIYENKKFYF PDMGKFYNKM VEKRLAEKFE AFLIQTRPNA RNNRLISFLN DNRIAERSFG GYSISETGLE SDRNPNSAGA INEVNKFIRD AFGNPYIPGS SLKGAIRTIL MNTTPKWNNE NAVNDFGRFP KENKNLIPWG PKKGKEYDDL FNAIRVSDSK PFDNKRLILV QKWDYSAKTN KAKPLPLYRE SISPLTKIEF EITTTTDEAG RLIEELGKRA QAFYKDYKAF FLSEFPDDKI QANLQYPIYL GAGSGAWTKT LFKQADGILQ RRYSRMKTKM VKKGVLKLTK APLKIVKIPS GNHSLIKNHE SFYEMGKANF MIKEIDK //