ID CSM5_STRTR Reviewed; 357 AA. AC A0A0A7HF79; DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot. DT 04-MAR-2015, sequence version 1. DT 08-MAY-2019, entry version 14. DE RecName: Full=CRISPR system Cms protein Csm5; DE AltName: Full=CRISPR type III A-associated protein Csm5; GN Name=csm5 {ECO:0000303|PubMed:25458845}; OS Streptococcus thermophilus. OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; OC Streptococcus. OX NCBI_TaxID=1308; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN PHAGE RESISTANCE, RP TARGETS SSRNA, SUBUNIT, AND ANTIVIRAL DEFENSE. RC STRAIN=DGCC8004; RX PubMed=25458845; DOI=10.1016/j.molcel.2014.09.027; RA Tamulaitis G., Kazlauskiene M., Manakova E., Venclovas C., RA Nwokeoji A.O., Dickman M.J., Horvath P., Siksnys V.; RT "Programmable RNA shredding by the type III-A CRISPR-Cas system of RT Streptococcus thermophilus."; RL Mol. Cell 56:506-517(2014). RN [2] RP SUBUNIT. RC STRAIN=DGCC8004; RX PubMed=27105119; DOI=10.1016/j.molcel.2016.03.024; RA Kazlauskiene M., Tamulaitis G., Kostiuk G., Venclovas C., Siksnys V.; RT "Spatiotemporal control of type III-A CRISPR-Cas immunity: coupling RT DNA degradation with the target RNA recognition."; RL Mol. Cell 62:295-306(2016). RN [3] RP SUBUNIT. RC STRAIN=DGCC8004; RX PubMed=28663439; DOI=10.1126/science.aao0100; RA Kazlauskiene M., Kostiuk G., Venclovas C., Tamulaitis G., Siksnys V.; RT "A cyclic oligonucleotide signaling pathway in type III CRISPR-Cas RT systems."; RL Science 357:605-609(2017). CC -!- FUNCTION: CRISPR (clustered regularly interspaced short CC palindromic repeat) is an adaptive immune system that provides CC protection against mobile genetic elements (viruses, transposable CC elements and conjugative plasmids). CRISPR clusters contain CC spacers, sequences complementary to antecedent mobile elements, CC and target invading nucleic acids. CRISPR clusters are transcribed CC and processed into CRISPR RNA (crRNA). The type III-A Csm effector CC complex binds crRNA and acts as a crRNA-guided RNase, DNase and CC cyclic oligoadenylate synthase; binding of target RNA cognate to CC the crRNA is required for all activities. In a heterologous host CC this Csm effector complex restricts ssRNA phage MS2, suggesting it CC may target RNA viruses in vivo. {ECO:0000269|PubMed:25458845}. CC -!- FUNCTION: Csm functions as a non-specific ssDNase. Base-pairing CC between crRNA and target RNA to form a ternary Csm complex CC activates a ssDNase activity; target RNA cleavage suppresses the CC ssDNase, a temporal control that prevents uncontrolled DNA CC degradation. Viral RNA transcripts probably tether the Csm complex CC to the viral genome, recruiting Cas10 ssDNA activity which is able CC to degrade DNA in the transcription bubble, spatially controlling CC the DNase activity. {ECO:0000269|PubMed:27105119}. CC -!- FUNCTION: This subunit might be involved in maturation of a crRNA CC intermediate to its mature form. {ECO:0000305|PubMed:25458845}. CC -!- SUBUNIT: Part of the Csm effector complex that includes at least CC Cas10(1), Csm2(3), Csm3(5), Csm4(1), Csm5(1) and mature crRNA CC (PubMed:25458845, PubMed:27105119, PubMed:28663439). The Csm CC complex is elongated and slightly twisted with a maximal length of CC 215 Angstroms and a diameter of 75-80 Angstroms (PubMed:25458845). CC It has been modeled to have a central protein filamant of Csm3 CC subunits along which the dsRNA helix of paired crRNA and target CC RNA binds. The filament is capped at one end by Cas10 and Csm4 and CC at the other end by Csm5; ssDNA is thought to bind to the N- CC terminal HD domain of Cas10 (Probable). Csm with a precursor crRNA CC does not include Csm5, while Cas6, the enzyme probably involved in CC pre-crRNA processing, is found associated with a subset of the Csm CC complex (PubMed:25458845). {ECO:0000269|PubMed:25458845, CC ECO:0000269|PubMed:27105119, ECO:0000269|PubMed:28663439, CC ECO:0000305|PubMed:25458845, ECO:0000305|PubMed:27105119}. CC -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus. CC {ECO:0000269|PubMed:25458845}. CC -!- SIMILARITY: Belongs to the CRISPR-associated Csm5 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KM222358; AIZ03608.1; -; Genomic_DNA. DR SMR; A0A0A7HF79; -. DR GeneID; 31937762; -. DR eggNOG; ENOG4105G15; Bacteria. DR eggNOG; COG1332; LUCA. DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW. DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW. DR InterPro; IPR010173; CRISPR-assoc_Csm5. DR InterPro; IPR005537; RAMP_III_fam. DR PANTHER; PTHR38007; PTHR38007; 1. DR Pfam; PF03787; RAMPs; 1. DR TIGRFAMs; TIGR01899; cas_TM1807_csm5; 1. PE 1: Evidence at protein level; KW Antiviral defense; RNA-binding. FT CHAIN 1 357 CRISPR system Cms protein Csm5. FT /FTId=PRO_0000446124. SQ SEQUENCE 357 AA; 41161 MW; 51C66D957D73F157 CRC64; MKNDYRTFKL SLLTLAPIHI GNGEKYTSRE FIYENKKFYF PDMGKFYNKM VEKRLAEKFE AFLIQTRPNA RNNRLISFLN DNRIAERSFG GYSISETGLE SDRNPNSAGA INEVNKFIRD AFGNPYIPGS SLKGAIRTIL MNTTPKWNNE NAVNDFGRFP KENKNLIPWG PKKGKEYDDL FNAIRVSDSK PFDNKRLILV QKWDYSAKTN KAKPLPLYRE SISPLTKIEF EITTTTDEAG RLIEELGKRA QAFYKDYKAF FLSEFPDDKI QANLQYPIYL GAGSGAWTKT LFKQADGILQ RRYSRMKTKM VKKGVLKLTK APLKIVKIPS GNHSLIKNHE SFYEMGKANF MIKEIDK //