ID A0A0A7AAX8_9RHAB Unreviewed; 2075 AA. AC A0A0A7AAX8; DT 04-MAR-2015, integrated into UniProtKB/TrEMBL. DT 04-MAR-2015, sequence version 1. DT 03-AUG-2022, entry version 40. DE RecName: Full=RNA-directed RNA polymerase L {ECO:0000256|ARBA:ARBA00018602}; DE EC=2.1.1.375 {ECO:0000256|ARBA:ARBA00026099}; DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494}; DE EC=2.7.7.88 {ECO:0000256|ARBA:ARBA00012582}; DE AltName: Full=Large structural protein {ECO:0000256|ARBA:ARBA00030285}; DE AltName: Full=Replicase {ECO:0000256|ARBA:ARBA00031012}; DE AltName: Full=Transcriptase {ECO:0000256|ARBA:ARBA00030436}; GN Name=L {ECO:0000313|EMBL:AHD46115.1}; OS Eel virus European X. OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; OC Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; OC Perhabdovirus. OX NCBI_TaxID=685443 {ECO:0000313|EMBL:AHD46115.1, ECO:0000313|Proteomes:UP000232555}; RN [1] {ECO:0000313|EMBL:AHD46115.1, ECO:0000313|Proteomes:UP000232555} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DK3631 {ECO:0000313|EMBL:AHD46115.1}; RA Meistertzheim A.-L., Galinier R., Morin T., Van beurden S., Schutze H., RA Sasal P., Faliex E.; RT "Phylogeny of Eel Virus American (EVA) and Eel Virus European X (EVEX), two RT fish rhabdoviruses: brother or not?"; RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; CC Evidence={ECO:0000256|ARBA:ARBA00001270}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)- CC adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a CC 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)- CC adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:65440, Rhea:RHEA-COMP:16798, Rhea:RHEA-COMP:16801, CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156482, CC ChEBI:CHEBI:156483; Evidence={ECO:0000256|ARBA:ARBA00024499}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl- CC adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N(7)-methyl CC 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl- CC adenosine in mRNA + H(+) + 2 S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:65376, Rhea:RHEA-COMP:16797, Rhea:RHEA-COMP:16798, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:156483, ChEBI:CHEBI:156484; EC=2.1.1.375; CC Evidence={ECO:0000256|ARBA:ARBA00024634}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl- CC adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'- CC triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl- CC adenosine in mRNA + H(+) + S-adenosyl-L-homocysteine; CC Xref=Rhea:RHEA:65380, Rhea:RHEA-COMP:16797, Rhea:RHEA-COMP:16801, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, CC ChEBI:CHEBI:156482, ChEBI:CHEBI:156484; CC Evidence={ECO:0000256|ARBA:ARBA00024452}; CC -!- CATALYTIC ACTIVITY: CC Reaction=a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in CC mRNA + GDP + H(+) = a 5'-end (5'-triphosphoguanosine)-adenylyl- CC adenylyl-cytidylyl-adenosine in mRNA + diphosphate; CC Xref=Rhea:RHEA:65436, Rhea:RHEA-COMP:16797, Rhea:RHEA-COMP:16799, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:58189, CC ChEBI:CHEBI:156484, ChEBI:CHEBI:156503; EC=2.7.7.88; CC Evidence={ECO:0000256|ARBA:ARBA00024494}; CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Virion CC {ECO:0000256|ARBA:ARBA00004328}. CC -!- SIMILARITY: Belongs to the rhabdoviridae protein L family. CC {ECO:0000256|ARBA:ARBA00006874}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; KC608035; AHD46115.1; -; Viral_cRNA. DR Proteomes; UP000232555; Genome. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. DR GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-KW. DR Gene3D; 3.40.50.150; -; 1. DR InterPro; IPR039530; L_methyltransferase_rhabdo. DR InterPro; IPR039736; L_poly_C. DR InterPro; IPR026890; Mononeg_mRNAcap. DR InterPro; IPR014023; Mononeg_RNA_pol_cat. DR InterPro; IPR025786; Mononega_L_MeTrfase. DR InterPro; IPR017234; RNA-dir_pol_rhabdovirus. DR InterPro; IPR029063; SAM-dependent_MTases_sf. DR Pfam; PF14314; Methyltrans_Mon; 1. DR Pfam; PF14318; Mononeg_mRNAcap; 1. DR Pfam; PF00946; Mononeg_RNA_pol; 1. DR PIRSF; PIRSF037546; RNA_pol_RhabdoV_sub; 1. DR TIGRFAMs; TIGR04198; paramyx_RNAcap; 1. DR PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1. DR PROSITE; PS51590; SAM_MT_MNV_L; 1. PE 3: Inferred from homology; KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603}; KW mRNA capping {ECO:0000256|ARBA:ARBA00023042}; KW mRNA processing {ECO:0000256|ARBA:ARBA00022664}; KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268}; KW Nucleotidyltransferase {ECO:0000313|EMBL:AHD46115.1}; KW RNA-directed RNA polymerase {ECO:0000313|EMBL:AHD46115.1}; KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}; KW Transferase {ECO:0000313|EMBL:AHD46115.1}; KW Virion {ECO:0000256|ARBA:ARBA00022844}. FT DOMAIN 590..776 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50526" FT DOMAIN 1623..1819 FT /note="Mononegavirus-type SAM-dependent 2'-O-MTase" FT /evidence="ECO:0000259|PROSITE:PS51590" SQ SEQUENCE 2075 AA; 236081 MW; 44304FCFFB3F210D CRC64; MYDEDYSRGY ESDDHYDLPE WLEEDIQSGN PLNQKDYSLN SPLIVDLTEA LVKYLNQGSI ERRFLRHQDR FENIQSEIRN IAWKNPSSSN HRWWGRWAQQ TEKSPEFVSL LSDVNQDIEE TSDVLMSFLK GWIQDTTSVP TKLNWTSTQL SYGSNFFFMH KLILFMNAQS DEERVILQGH IKVSETKKTG IYKGSHPSLG NFVLTSEFLL LERHRVILDR SFLLMVKDTL VGRFQTLASF MNREDKKYPE DVIEKVETLY SLGDQLVEDL GDEAYSGIKL LEPACNLRLA ELAREFRPLI PEFPHFRNHV ETAIAEESVF SPGITEFFSH VNKETNVEII LAYFSSFRHW GHPYIDYFQG LIKLNKQVTL EKDIDTEYAN ALASDLAYMI LRGHFNTKRV WAVDKSLVSK QHPLSEHIFN ATWPTPKQID DFGDHWHELP LIKIYDIPDL IDPSVIYSDK SHSMGREEVL KHVQRNPTQA IPTKKVLETL LQKPATNWPE FLSSIEKDGL PKDSLIIGLK GKERELKKAG RFFSLMSWEL REYFVITEYL IKTHYVPLFK GLTMADDMTE VVKKMLERSQ GQGEDDYEHV SIANHIDYEK WNNHQRKESN GPVFRVMGQF PGHPSLIEKT HDFFEQSLIY YNGRPDLMQT DGDELQNRTE ALVCWNGQKG GLEGLRQKGW SILNLLVIKR ESKIRNTKVQ TLAQGDNQVV CTQYRIMPTR STLELQAELE KVKKNNQVIM DAIETGTNKL GLLINNDETI QSADFLTYGK VPIFRGNIRC LETKRWSRVT CVTNDQLPSL SNVMSSVSTN SLTVSHFDVS PIESMRQYLF FGNFARRLVE FHNPAMRVPI SLEDLDSKQK SVYLNAVLFL DPSLGGVSGM SLSRFLTRMF PDPITEGLSF WKIVYEHTTS KDTQLLCRIA GSPELARRQN NLDKLIENPT ALNLSKETSA LSVIKKEVRS RLYKDCDKFK NKLIADAIGI ARDEEAHLEL FLMSIRPLFP RFLAEFKAAT FVGITESLIS LFQNSKTIRN IFRKKYAKEL ELRVVQCEYR SINLMLSLAD RSHLDEMWTC SASKADELRT LSWGTTIIGT TVPHPLEMIN HAHIGQKCDS LETLDYINVT VVQDLTDCLT SKGKLPAYLG SKTSETTSIL QPWEKETKIP VIRRAAKLRA AITWFVEPDS LLAQSILNNI ESLTGEDWSA SISGFKRTGS ALHRFTSARV SAGGFSAQSP ARLTRMMATT DTFREIGSDN YDFMFQSLLL FAQMTTGEIY KRSPATNFHF HLSCHQCLRK IEEPTLNSDF AYNPIQRSDI LDKWKPQTTD WSSERKAPEI EEGNWDRLTH QEQSFQIGKS IGFLFGDLTM TKNSHAQDSS IFPLSIQYKI TAAEFLEGIL DGIVKASALS TIHRRNFDHH SKYKSTVSGT VDYLIELISE SAGFTNLTRN GPLKACLLTI PHKIPPSYPL SQSDLGAMSR NYLRLLHRRM STGTYKTRWP TNWIFSDMMS PNIIYPFVIS VSCVGLAYSS SWTKKSADKL RGLRGVAELI RSSDDVQLPV GKLFKTVNQE IRHAIKHHAS DDAEIPESHI QEGWKKELIV NINMQPIDYS RTATAKSLDR PAQIRDPLIS GLRTAQLATG SHYKLRSILV QNRIQVTDAL CGGDGSGGIG ACVLRQYPFA KLIYNSLFEI QDLDMRGSAP GPPSAIAAMG NMSMRCVNRD SAWKNPSDLS HTSTWEYFQS LMTQHQLRCN LWTFDMEVRS HDISDAIEEQ IVANLHLLPK NGTIIYKTYL TKLSDMETTI LDRLGGFFKR VSLVSTDATS SHSSEVYAVF QNKLDKRQLE IHPNWSSCNL GSDIHPCWKS EDEEFERARR FFHMKRQQGV PTRLRPTLDS EIQVLSVSAG VENGVAMTLA MDVSNQVSDP TTGAFLWLLV TLQHICPIGP SFKIPSSVAV ESYLAILIGF SSIYQLQTGS NKAYASIKTC LSQSAPFFCN PDGWSCIKGL DKSMRMDRKL ALVGSVIRAW SKWNLTQSIN FRKLDGMMRH YLPKGTLRQI GCKTGIWDYI NGAVKGVRSA ANQDSPKESA AAWRD //