ID A0A0A2WM79_THEFI Unreviewed; 321 AA. AC A0A0A2WM79; DT 04-FEB-2015, integrated into UniProtKB/TrEMBL. DT 04-FEB-2015, sequence version 1. DT 03-AUG-2022, entry version 28. DE RecName: Full=Holliday junction ATP-dependent DNA helicase RuvB {ECO:0000256|HAMAP-Rule:MF_00016}; DE EC=3.6.4.12 {ECO:0000256|HAMAP-Rule:MF_00016}; GN Name=ruvB {ECO:0000256|HAMAP-Rule:MF_00016}; GN ORFNames=THFILI_03480 {ECO:0000313|EMBL:KGQ20923.1}; OS Thermus filiformis. OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus. OX NCBI_TaxID=276 {ECO:0000313|EMBL:KGQ20923.1, ECO:0000313|Proteomes:UP000030364}; RN [1] {ECO:0000313|EMBL:KGQ20923.1, ECO:0000313|Proteomes:UP000030364} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 43280 {ECO:0000313|EMBL:KGQ20923.1, RC ECO:0000313|Proteomes:UP000030364}; RA Mandelli F., Ramires B.O., Paixao D.A., Camilo C.M., Polikarpov I., RA Couger M.B., Prade R., Riano-Pachon D.M., Squina F.M.; RT "Draft Genome Sequence of the thermophile Thermus filiformis ATCC43280 RT producer of carotenoid-(di)glucoside-branched fatty acids (di)esters."; RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: The RuvA-RuvB complex in the presence of ATP renatures CC cruciform structure in supercoiled DNA with palindromic sequence, CC indicating that it may promote strand exchange reactions in homologous CC recombination. RuvAB is a helicase that mediates the Holliday junction CC migration by localized denaturation and reannealing. CC {ECO:0000256|HAMAP-Rule:MF_00016}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC Evidence={ECO:0000256|HAMAP-Rule:MF_00016}; CC -!- SUBUNIT: Forms a complex with RuvA. {ECO:0000256|HAMAP-Rule:MF_00016}. CC -!- SIMILARITY: Belongs to the RuvB family. {ECO:0000256|HAMAP- CC Rule:MF_00016}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KGQ20923.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JPSL02000037; KGQ20923.1; -; Genomic_DNA. DR STRING; 276.THFILI_03480; -. DR EnsemblBacteria; KGQ20923; KGQ20923; THFILI_03480. DR PATRIC; fig|276.5.peg.2298; -. DR Proteomes; UP000030364; Unassembled WGS sequence. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA. DR GO; GO:0003677; F:DNA binding; IEA:InterPro. DR GO; GO:0009378; F:four-way junction helicase activity; IEA:UniProtKB-UniRule. DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule. DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule. DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule. DR Gene3D; 1.10.10.10; -; 1. DR Gene3D; 3.40.50.300; -; 1. DR HAMAP; MF_00016; DNA_helic_RuvB; 1. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR041445; AAA_lid_4. DR InterPro; IPR004605; DNA_helicase_Holl-junc_RuvB. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR008824; RuvB-like_N. DR InterPro; IPR008823; RuvB_C. DR InterPro; IPR036388; WH-like_DNA-bd_sf. DR InterPro; IPR036390; WH_DNA-bd_sf. DR PANTHER; PTHR42848; PTHR42848; 1. DR Pfam; PF17864; AAA_lid_4; 1. DR Pfam; PF05491; RuvB_C; 1. DR Pfam; PF05496; RuvB_N; 1. DR SMART; SM00382; AAA; 1. DR SUPFAM; SSF46785; SSF46785; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00635; ruvB; 1. PE 3: Inferred from homology; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP- KW Rule:MF_00016}; DNA damage {ECO:0000256|HAMAP-Rule:MF_00016}; KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP- KW Rule:MF_00016}; DNA repair {ECO:0000256|HAMAP-Rule:MF_00016}; KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|HAMAP-Rule:MF_00016}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00016}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP- KW Rule:MF_00016}; SOS response {ECO:0000256|HAMAP-Rule:MF_00016}. FT DOMAIN 36..168 FT /note="AAA" FT /evidence="ECO:0000259|SMART:SM00382" FT BINDING 44..51 FT /ligand="ATP" FT /ligand_id="ChEBI:CHEBI:30616" FT /evidence="ECO:0000256|HAMAP-Rule:MF_00016" SQ SEQUENCE 321 AA; 35849 MW; ED15CF062B47F245 CRC64; MEPALRPKTL DEYVGQERLK KKLRVYLEAA KARKEPLDHL LLVGPPGLGK TTLAHIIAYE LGVKIRVTSG PAIEKPGDLA AILTNSLDEG DVLFIDEIHR LSRQAEEHLY PAMEDFKMDI VIGQGPAART LRLDLPRFTL IGATTRPGLL TSPLRSRFGI VEHLEFYTVE ELAQAIRRDA ALLGLEVEEE AAQEMGRRSR GTMRIAKRLF RRVRDFAEVA GERRITLERT LEALRALGLD ELGLEKRDRE ILETLILRFG GGPVGLVTLA TALAEDPGTL EEVYEPYLIQ LGLLKRTPRG RVATEAAYRH LGFALPMDPL L //