ID A0A0A0FA54_ECOLX Unreviewed; 516 AA. AC A0A0A0FA54; DT 07-JAN-2015, integrated into UniProtKB/TrEMBL. DT 07-JAN-2015, sequence version 1. DT 02-JUN-2021, entry version 20. DE RecName: Full=Cyclic-guanylate-specific phosphodiesterase {ECO:0000256|ARBA:ARBA00012282}; DE EC=3.1.4.52 {ECO:0000256|ARBA:ARBA00012282}; GN ORFNames=EL76_3599 {ECO:0000313|EMBL:KGM60011.1}; OS Escherichia coli G3/10. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=1455601 {ECO:0000313|EMBL:KGM60011.1, ECO:0000313|Proteomes:UP000030015}; RN [1] {ECO:0000313|EMBL:KGM60011.1, ECO:0000313|Proteomes:UP000030015} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=G3/10 {ECO:0000313|EMBL:KGM60011.1, RC ECO:0000313|Proteomes:UP000030015}; RA Zschuettig A., Auerbach C., Meltke S., Eichhorn C., Brandt M., Blom J., RA Goesmann A., Jarek M., Scharfe M., Zimmermann K., Wassenaar T.M., RA Gunzer F.; RT "Complete sequence of probiotic Symbioflor2 E. coli strain G3/10 and draft RT sequences of Symbioflor2 strains G1/2, G4/9, G5, G6/7 and G8."; RL Submitted (JUL-2014) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=cyclic di-3',5'-guanylate + H2O = 5'- CC phosphoguanylyl(3'->5')guanosine + H(+); Xref=Rhea:RHEA:24902, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58754, CC ChEBI:CHEBI:58805; EC=3.1.4.52; CC Evidence={ECO:0000256|ARBA:ARBA00001196}; CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ CC whole genome shotgun (WGS) entry which is preliminary data. CC {ECO:0000313|EMBL:KGM60011.1}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; JPKI01000003; KGM60011.1; -; Genomic_DNA. DR RefSeq; WP_001299455.1; NZ_JPKI01000003.1. DR EnsemblBacteria; KGM60011; KGM60011; EL76_3599. DR GeneID; 61752354; -. DR PATRIC; fig|1455601.3.peg.3631; -. DR Proteomes; UP000030015; Unassembled WGS sequence. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW. DR GO; GO:0071111; F:cyclic-guanylate-specific phosphodiesterase activity; IEA:UniProtKB-EC. DR CDD; cd01948; EAL; 1. DR Gene3D; 3.20.20.450; -; 1. DR InterPro; IPR024744; CSS-motif_dom. DR InterPro; IPR001633; EAL_dom. DR InterPro; IPR035919; EAL_sf. DR Pfam; PF12792; CSS-motif; 1. DR Pfam; PF00563; EAL; 1. DR SMART; SM00052; EAL; 1. DR SUPFAM; SSF141868; SSF141868; 1. DR PROSITE; PS50883; EAL; 1. PE 4: Predicted; KW c-di-GMP {ECO:0000256|ARBA:ARBA00022636}; KW Cell membrane {ECO:0000256|ARBA:ARBA00022475}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}. FT TRANSMEM 243..265 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 268..516 FT /note="EAL" FT /evidence="ECO:0000259|PROSITE:PS50883" SQ SEQUENCE 516 AA; 58800 MW; 130433200A3C2F50 CRC64; MRTRHLVGLI SGVLILSVLL PVGLSIWLAH QQVETSFIEE LDTYSSRVAI RANKVATQGK DALQELERWQ GAACSEAHLM EMRRVSYSYR YIQEVVYIDN NVPQCSSLEH ESPPDTFPEP GKISKDGYRV WLTSHNDLGI IRYMVAMGTA HYVVMIDPAS FIDVIPYSSW QIDAAIIGNA HNVVITSSDE IAQGIITRLQ KTPGEHIENN GIIYDILPFP EMNISIITWA STKMLQKGWH RQVFIWLPLG LVIGLLAAMF VLRILRRIQS PHHRLQDAIE NRDICVHYQP IVSLANGKIV GAEALARWPQ TDGSWLSPDS FIPLAQQTGL SEPLTLLIIR SVFEDMGDWL RQHPQQHISI NLESTVLTSE KIPQLLREMI NHYQVNPRQI ALELTEREFA DPKTSAPIIS RYREAGHEIY LDDFGTGYSS LSYLQDLDVD ILKIDKSFVD ALEYKNVTPH IIEMAKTLKL KMVAEGIETS KQEEWLRQHG VHYGQGWLYS KALPKEDFLR WAEQHL //